Immunofluorescence staining of mouse splenocytes using anti-CD22 antibody OX-97. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of OX-97 (MBS488992) at 10 µg/ml followed by Alexa Fluor 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488992, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.

CD22 Antibody

Name: CD22 Antibody; Mouse-Reactive; Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS488992
Price: 635 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS488992

This antibody was prepared by immunizing PVG rats.This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques. More details

$635 / 0.2 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD22, Antibody

Full Product Name

Anti-CD22 [OX-97]

Product Gene Name

anti-CD22 antibody

Product Synonym Names

Lyb-8; BL-CAM; B-cell receptor CD22; B-lymphocyte cell adhesion molecule; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; OX97; MRC OX 97;

Isotype

Rabbit IgG kappa

Clone Number

OX-97

Species Reactivity

Mouse

Form/Format

PBS with 0.02% Proclin 300.

Purity/Purification

Purified. Protein A affinity purified

Concentration

1mg/ml (lot specific)

Specificity

This antibody recognizes domain 2 of murine CD22, which is expressed on the surface of a subpopulation of mature B lymphocytes.

Preparation and Storage

Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Molecular Weight

92,857 Da

3D Structure

ModBase 3D Structure for P35329

Animal-free Manufacturing Platform

The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.

Product Categories/Family for anti-CD22 antibody

immunology; adaptive immunity; CD Markers

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-CD22 antibody

Flow Cytometry (FC/FACS), Western Blot (WB)

Other Notes

Small volumes of anti-CD22 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CD22 purchase

MBS488992 is an antibody targeting CD22. Also known as Anti-CD22 [OX-97]. Reactive in Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for CD22. It may not necessarily be applicable to this product.

NCBI GI #

461664

NCBI GeneID

12483

NCBI Accession #

P35329.1

UniProt Primary Accession #

P35329

UniProt Secondary Accession #

Q9JHL2; Q9JJX9; Q9JJY0; Q9JJY1; Q9R056; Q9R094; Q9WU51

UniProt Related Accession #

P35329

NCBI Official Full Name

B-cell receptor CD22

NCBI Official Synonym Full Names

CD22 antigen

NCBI Official Symbol

Cd22

NCBI Official Synonym Symbols

Lyb8; Lyb-8; A530093D23

UniProt Protein Name

B-cell receptor CD22

UniProt Synonym Protein Names

B-lymphocyte cell adhesion molecule; BL-CAM; Sialic acid-binding Ig-like lectin 2; Siglec-2; T-cell surface antigen Leu-14; CD_antigen: CD22

Protein Family

B-cell receptor

UniProt Gene Name

Cd22

UniProt Synonym Gene Names

Lyb-8; Siglec2; BL-CAM; Siglec-2

UniProt Entry Name

CD22_MOUSE

NCBI Protein Information

B-cell receptor CD22; BL-CAM; siglec-2; T-cell surface antigen Leu-14; B-lymphocyte cell adhesion molecule; sialic acid-binding Ig-like lectin 2

UniProt Comments for CD22

CD22: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell surface

Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; coreceptor activity; carbohydrate binding

Biological Process: cell surface receptor linked signal transduction; cell adhesion

Research Articles on CD22

1. These data assign a key role to CCL22 in Treg recruitment in the protection of NOD mice against type 1 diabetes following the treatment with G-CSF.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CD22 antibody

Products by Pathway	Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway antibodies	XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway Diagram

Diseases associated with anti-CD22 antibody

Disease Name	Pubmed Publications
Immune System Diseases Antibodies	>950 publications with CD22 and Immune System Diseases
Neoplasms, Experimental Antibodies	>105 publications with CD22 and Neoplasms, Experimental
Inflammation Antibodies	>56 publications with CD22 and Inflammation
Necrosis Antibodies	>51 publications with CD22 and Necrosis
Respiratory Tract Diseases Antibodies	>45 publications with CD22 and Respiratory Tract Diseases
Cell Transformation, Neoplastic Antibodies	>23 publications with CD22 and Cell Transformation, Neoplastic
Kidney Diseases Antibodies	>21 publications with CD22 and Kidney Diseases
Liver Diseases Antibodies	>19 publications with CD22 and Liver Diseases
Lung Diseases Antibodies	>17 publications with CD22 and Lung Diseases
Lung Neoplasms Antibodies	>15 publications with CD22 and Lung Neoplasms

Organs/Tissues associated with anti-CD22 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>1042 publications with CD22 and Blood
Bone Marrow Antibodies	>286 publications with CD22 and Bone Marrow
Spleen Antibodies	>121 publications with CD22 and Spleen
Lymph Node Antibodies	>110 publications with CD22 and Lymph Node
Skin Antibodies	>47 publications with CD22 and Skin
Embryonic Tissue Antibodies	>13 publications with CD22 and Embryonic Tissue
Nasopharynx Antibodies	>5 publications with CD22 and Nasopharynx
Inner Ear Antibodies	>1 publications with CD22 and Inner Ear