Interleukin 25 (IL25) CLIA Kit for Mouse

Name: Mouse IL25 CLIA Kit; Interleukin 25; MyBioSource
Brand: MyBioSource
SKU: MBS2001206
Price: 490 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2001206

The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 25 (IL25). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 25 (IL25). Next,… More details

$490 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Interleukin 25 (IL25), CLIA Kit

Full Product Name

Mouse Interleukin 25 (IL25) CLIA Kit

Product Gene Name

IL25 clia kit

Product Synonym Names

IL17E; IL17-E; Interleukin-17E

Species Reactivity

Mouse

Assay Type

Double-antibody Sandwich

Samples

Serum, Plasma and other biological fluids

Detection Range

1.37-1,000pg/mL

Sensitivity

0.56pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 25 (IL25).
No significant cross-reactivity or interference between Interleukin 25 (IL25) and analogues was observed.

Preparation and Storage

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Molecular Weight

18,523 Da

OMIM

605658

3D Structure

ModBase 3D Structure for Q9H293

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Application

Chemiluminescent immunoassay for Antigen Detection.

Intra-assay Precision (Precision within an assay)

3 samples with low, middle and high level Interleukin 25 (IL25) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays)

3 samples with low, middle and high level Interleukin 25 (IL25) were tested on 3 different plates, 8 replicates in each plate.

CV(%) =

SD/meanX100

Intra-Assay

CV<10%

Inter-Assay

CV<12%

Product Categories/Family for IL25 clia kit

Cytokine; Infection immunity

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of IL25 clia kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for IL25 purchase

MBS2001206 is a CLIA kit targeting IL25. Also known as Mouse Interleukin 25 (IL25) CLIA Kit. Reactive in Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for IL25. It may not necessarily be applicable to this product.

NCBI GI #

27477081

NCBI GeneID

64806

NCBI Accession #

NP_758525.1

UniProt Primary Accession #

Q9H293

UniProt Secondary Accession #

Q2M3F0; Q8IZV3; Q8WXB0

UniProt Related Accession #

Q9H293

GenBank Nucleotide #

NM_172314.1

NCBI Official Full Name

interleukin-25 isoform 2

NCBI Official Synonym Full Names

interleukin 25

NCBI Official Symbol

IL25

NCBI Official Synonym Symbols

IL17E

UniProt Protein Name

Interleukin-25

UniProt Synonym Protein Names

Interleukin-17E; IL-17E

Protein Family

Interleukin

UniProt Gene Name

IL25

UniProt Synonym Gene Names

IL17E; IL-25; IL-17E

UniProt Entry Name

IL25_HUMAN

NCBI Protein Information

interleukin-25

NCBI Summary for IL25

The protein encoded by this gene is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. Studies of a similar gene in mice suggest that this cytokine may be a pro-inflammatory cytokine favoring the Th2-type immune response. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

UniProt Comments for IL25

IL25: Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. Proinflammatory cytokine favoring Th2-type immune responses. Belongs to the IL-17 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space

Molecular Function: cytokine activity; interleukin-17E receptor binding

Biological Process: response to fungus; inflammatory response to antigenic stimulus; response to nematode; eosinophil differentiation; positive regulation of transcription from RNA polymerase II promoter

Research Articles on IL25

1. asthmatic epithelial cells have an increased intrinsic capacity for expression of a pro-type 2 cytokine in response to a viral infection, and IL-25 is a key mediator of RV-induced exacerbations of pulmonary inflammation

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with IL25 clia kit

Reference Product	PubMed Publications
IL33 clia kit	>11 publications with IL25 and IL33
ITGAX clia kit	>2 publications with IL25 and ITGAX
IL17RB clia kit	>2 publications with IL25 and IL17RB
IL23A clia kit	>1 publications with IL25 and IL23A

Pathways associated with IL25 clia kit

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
IL17 Signaling Pathway antibodies	IL17 Signaling Pathway Diagram

Diseases associated with IL25 clia kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>28 publications with IL25 and Inflammation
Hypersensitivity Antibodies	>24 publications with IL25 and Hypersensitivity
Lung Diseases Antibodies	>12 publications with IL25 and Lung Diseases
Skin Diseases Antibodies	>8 publications with IL25 and Skin Diseases
Hyperplasia Antibodies	>7 publications with IL25 and Hyperplasia
Disease Models, Animal Antibodies	>4 publications with IL25 and Disease Models, Animal
Colitis Antibodies	>4 publications with IL25 and Colitis
Necrosis Antibodies	>4 publications with IL25 and Necrosis
Pneumonia Antibodies	>2 publications with IL25 and Pneumonia
Leukocytosis Antibodies	>1 publications with IL25 and Leukocytosis