Alpha-Endorphin (aEP) ELISA Kit for Mouse

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Alpha-Endorphin (aEP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Alpha-Endorphin (aEP) and unlabeled Alpha-Endorphin (aEP) (Standards or samples) with the pre-coated antibody specific to Alpha-Endorphin (aEP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Alpha-Endorphin (aEP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Alpha-Endorphin (aEP) in the sample.

Small volumes of aEP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2700274 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Alpha-Endorphin (aEP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing aEP. The ELISA analytical biochemical technique of the MBS2700274 kit is based on aEP antibody-aEP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect aEP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, aEP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).