Complement Component 4 (C4) ELISA Kit for Mouse

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to C4. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to C4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of C4 in mouse serum, plasma and other biological fluids.

Small volumes of C4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2702660 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement Component 4 (C4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C4. The ELISA analytical biochemical technique of the MBS2702660 kit is based on C4 antibody-C4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).