Complement Fragment 4d, C4d (C4D) ELISA Kit for Mouse

Principle of the Assay: C4d ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-C4d antibody and an C4d-HRP conjugate. The assay sample and buffer are incubated together with C4d-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C4d concentration since C4d from samples and C4d-HRP conjugate compete for the anti-C4d antibody binding site. Since the number of sites is limited, as more sites are occupied by C4d from the sample, fewer sites are left to bind C4d-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C4d concentration in each sample is interpolated from this standard curve.

Intended Uses: This C4d ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse C4d. This ELISA kit for research use only, not for therapeutic or test applications!

Small volumes of C4d elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS7220356 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Complement Fragment 4d, C4d, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing C4d. The ELISA analytical biochemical technique of the MBS7220356 kit is based on C4d antibody-C4d antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect C4d antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, C4d. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).