Osteocalcin (OC) ELISA Kit for Mouse

Name: Mouse OC ELISA Kit; Osteocalcin; MyBioSource
Brand: MyBioSource
SKU: MBS2087398
Price: 460 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2087398

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of osteocalcin in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. More details

$460 / 24-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Osteocalcin (OC), ELISA Kit

Full Product Name

Mouse Osteocalcin (OC) Mini Samples ELISA Kit

Product Gene Name

OC elisa kit

Product Synonym Names

BGLAP; OT; BGP; Bone Gla Protein; Bone Gamma-Carboxyglutamate Protein; Gamma-carboxyglutamic acid-containing protein

Species Reactivity

Mouse

Assay Type

Double-antibody Sandwich

Samples

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids

Detection Range

0.156-10ng/mL

Sensitivity

0.057ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of osteocalcin. No significant cross-reactivity or interference between osteocalcin and analogues was observed.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level osteocalcin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level osteocalcin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

10,549 Da

3D Structure

ModBase 3D Structure for P86546

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Product Categories/Family for OC elisa kit

Metabolic pathway; Endocrinology; Hormone metabolism; Bone metabolism

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of OC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OC purchase

MBS2087398 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Osteocalcin (OC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OC. The ELISA analytical biochemical technique of the MBS2087398 kit is based on OC antibody-OC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for OC. It may not necessarily be applicable to this product.

NCBI GI #

6671634

NCBI GeneID

12096

NCBI Accession #

NP_031567.1

UniProt Primary Accession #

P86546

UniProt Secondary Accession #

P04641

UniProt Related Accession #

P86546

GenBank Nucleotide #

NM_007541.3

NCBI Official Full Name

osteocalcin preproprotein

NCBI Official Synonym Full Names

bone gamma carboxyglutamate protein

NCBI Official Symbol

Bglap

NCBI Official Synonym Symbols

OC; BGP; OG1; mOC-A; Bglap1

UniProt Protein Name

Osteocalcin

UniProt Synonym Protein Names

Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein

UniProt Gene Name

Bglap

UniProt Synonym Gene Names

BGP

NCBI Protein Information

osteocalcin

NCBI Summary for OC

This gene encodes one of the most abundant non-collagenous proteins in bone tissue that is localized to the mineralized matrix of bone. The encoded preproprotein undergoes proteolytic processing and post-translational gamma carboxylation to generate a mature, calcium-binding protein. Mice lacking the encoded protein develop abnormalities of bone remodelling. This gene is located adjacent to two other osteocalcin-related genes on chromosome 3. [provided by RefSeq, Oct 2015]

UniProt Comments for OC

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.

Research Articles on OC

1. expression in offspring bone not changed by maternal high fat diet

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with OC elisa kit

Products by Pathway	Pathway Diagram
Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway antibodies	Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway Diagram
Gamma-carboxylation Of Protein Precursors Pathway antibodies	Gamma-carboxylation Of Protein Precursors Pathway Diagram
Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway antibodies	Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
Post-translational Protein Modification Pathway antibodies	Post-translational Protein Modification Pathway Diagram
Removal Of Aminoterminal Propeptides From Gamma-carboxylated Proteins Pathway antibodies	Removal Of Aminoterminal Propeptides From Gamma-carboxylated Proteins Pathway Diagram
Transport Of Gamma-carboxylated Protein Precursors From The Endoplasmic Reticulum To The Golgi Apparatus Pathway antibodies	Transport Of Gamma-carboxylated Protein Precursors From The Endoplasmic Reticulum To The Golgi Apparatus Pathway Diagram

Diseases associated with OC elisa kit

Disease Name	Pubmed Publications
Bone Diseases Antibodies	>17 publications with OC and Bone Diseases
Osteoporosis Antibodies	>12 publications with OC and Osteoporosis
Inflammation Antibodies	>6 publications with OC and Inflammation
Nervous System Diseases Antibodies	>4 publications with OC and Nervous System Diseases
Cardiovascular Diseases Antibodies	>3 publications with OC and Cardiovascular Diseases
Kidney Diseases Antibodies	>3 publications with OC and Kidney Diseases
Insulin Resistance Antibodies	>3 publications with OC and Insulin Resistance
Calcinosis Antibodies	>3 publications with OC and Calcinosis
Necrosis Antibodies	>2 publications with OC and Necrosis
Hypertrophy Antibodies	>2 publications with OC and Hypertrophy

Organs/Tissues associated with OC elisa kit

Organ/Tissue Name	Pubmed Publications
Embryonic Tissue Antibodies	>3 publications with OC and Embryonic Tissue
Joint Antibodies	>2 publications with OC and Joint
Intestine Antibodies	>1 publications with OC and Intestine