Paxillin (PXN) ELISA Kit for Mouse

Name: Mouse PXN ELISA Kit; Paxillin; MyBioSource
Brand: MyBioSource
SKU: MBS2805597
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2805597

MBS2805597 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Paxillin (PXN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or… More details

$495 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Paxillin (PXN), ELISA Kit

Full Product Name

Mouse Paxillin (PXN) ELISA Kit

Product Gene Name

PXN elisa kit

Product Synonym Names

hCG_1778014; FLJ16691

Species Reactivity

Mouse

Assay Type

Quantitive Sandwich

Samples

Serum, Plasma, Other biological fluids

Detection Range

62.5-4000pg/mL

Sensitivity

31.25pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Mouse PXN. No significant cross-reactivity or interference between Mouse PXN and analogues was observed.

Preparation and Storage

Store at 2-8 degree C. Please refer to Instruction Manual.

Molecular Weight

64,476 Da

Chromosome Location

Chromosome: 5; NC_000071.6 (115506702..115555986). Location: 5 F; 5 56.1 cM

3D Structure

ModBase 3D Structure for Q8VI36

Detection Wavelength

450nm

Assay Duration

1-4.5h

Sample Volume

1-200uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV

Test Principle

This assay employs a two-site sandwich ELISA to quantitate PXN in samples. An antibody specific for PXN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PXN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXN bound in the initial step. The color development is stopped and the intensity of the color is measured.

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of PXN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PXN. It may not necessarily be applicable to this product.

NCBI GI #

114326502

NCBI GeneID

19303

NCBI Accession #

NP_598676.2

UniProt Primary Accession #

Q8VI36

UniProt Secondary Accession #

Q3TB62; Q3TZQ6; Q8VI37

UniProt Related Accession #

Q8VI36

GenBank Nucleotide #

NM_133915.3

NCBI Official Full Name

paxillin isoform beta

NCBI Official Synonym Full Names

paxillin

NCBI Official Symbol

Pxn

NCBI Official Synonym Symbols

Pax; AW108311; AW123232

UniProt Protein Name

Paxillin

Protein Family

Paxillin

UniProt Gene Name

Pxn

UniProt Entry Name

PAXI_MOUSE

NCBI Protein Information

paxillin

UniProt Comments for PXN

Function: Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion)

By similarity.

Subunit structure: Interacts with VCL and SRC (via SH3 domain). Interacts with GIT1, NUDT16L1/SDOS and TGFB1I1. Component of cytoplasmic complexes, which also contain GIT1, ARHGEF6 and PAK1. Binds ASAP2. Interacts with RNF5 and PDCD10

By similarity. Interacts with SORBS1, PARVA and PARVB. Interacts with NEK3 and this interaction is prolactin-dependent

By similarity. Interacts with PTK2/FAK1. Interacts with PTK6

By similarity. Interacts with PTK2B/PYK2. Ref.3 Ref.5 Ref.6

Subcellular location: Cytoplasm › cytoskeleton

By similarity. Cell junction › focal adhesion

By similarity. Cytoplasm › cell cortex. Note: Colocalizes with integrins at the cell periphery. Ref.3

Post-translational modification: Phosphorylated by MAPK1/ERK2. Phosphorylated on tyrosine residues during integrin-mediated cell adhesion, embryonic development, fibroblast transformation and following stimulation of cells by mitogens. Phosphorylation at Ser-244 by CDK5 reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation

By similarity. Phosphorylation at Tyr-31 and Tyr-118 by PTK6 promote the activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion

By similarity. Ref.4 Ref.10

Sequence similarities: Belongs to the paxillin family.Contains 4 LIM zinc-binding domains.

Research Articles on PXN

1. Paxillin is critical for integrating physical cues from the ECM with chemical motility signals by spatially constraining where cells form motile processes, and thereby regulates directional migration.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with PXN elisa kit

Products by Pathway	Pathway Diagram
Androgen Receptor Signaling Pathway antibodies	Androgen Receptor Signaling Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Cell Junction Organization Pathway antibodies	Cell Junction Organization Pathway Diagram
Cell-Cell Communication Pathway antibodies	Cell-Cell Communication Pathway Diagram
Cell-extracellular Matrix Interactions Pathway antibodies	Cell-extracellular Matrix Interactions Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Disease Pathway antibodies	Disease Pathway Diagram

Diseases associated with PXN elisa kit

Disease Name	Pubmed Publications
Neoplasm Metastasis Antibodies	>39 publications with PXN and Neoplasm Metastasis
Breast Neoplasms Antibodies	>31 publications with PXN and Breast Neoplasms
Inflammation Antibodies	>24 publications with PXN and Inflammation
Gastrointestinal Diseases Antibodies	>24 publications with PXN and Gastrointestinal Diseases
Adenocarcinoma Antibodies	>20 publications with PXN and Adenocarcinoma
Neoplasms, Experimental Antibodies	>17 publications with PXN and Neoplasms, Experimental
Necrosis Antibodies	>16 publications with PXN and Necrosis
Cardiovascular Diseases Antibodies	>14 publications with PXN and Cardiovascular Diseases
Ovarian Neoplasms Antibodies	>11 publications with PXN and Ovarian Neoplasms
Vascular Diseases Antibodies	>11 publications with PXN and Vascular Diseases

Organs/Tissues associated with PXN elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>106 publications with PXN and Blood
Muscle Antibodies	>68 publications with PXN and Muscle
Lung Antibodies	>44 publications with PXN and Lung
Kidney Antibodies	>38 publications with PXN and Kidney
Embryonic Tissue Antibodies	>29 publications with PXN and Embryonic Tissue
Brain Antibodies	>28 publications with PXN and Brain
Bone Antibodies	>24 publications with PXN and Bone
Eye Antibodies	>19 publications with PXN and Eye
Liver Antibodies	>19 publications with PXN and Liver
Skin Antibodies	>16 publications with PXN and Skin