Protein Tob1 (TOB1) ELISA Kit for Mouse

Description: This Mouse Protein Tob1 (TOB1) ELISA Kit employs a two-site sandwich ELISA to quantitate TOB1 in samples. An antibody specific for TOB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TOB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: TOB1 encodes a member of the tob/btg1 family of anti-proliferative proteins that have the potential to regulate cell growth. When exogenously expressed, this protein supresses cell growth in tissue culture. The protein undergoes phophorylation by a serine/threonine kinase, 90 kDa ribosomal S6 kinase. Interactions of this protein with the v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 gene product p185 interferes with growth suppression. This protein inhibits T cell proliferation and transcription of cytokines and cyclins. The protein interacts with both mothers against decapentaplegic Drosophila homolog 2 and 4 to enhance their DNA binding activity. This interaction inhibits interleukin 2 transcription in T cells.

Small volumes of TOB1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9716694 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Protein Tob1 (TOB1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing TOB1. The ELISA analytical biochemical technique of the MBS9716694 kit is based on TOB1 antibody-TOB1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect TOB1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, TOB1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).