Serpine2 ELISA Kit for Mouse

Principle of the Assay: The Picokine™ Mouse Serpine2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Serpine2 with a 96-well strip plate that is pre-coated with antibody specific for Serpine2. The detection antibody is a biotinylated antibody specific for Serpine2. The capture antibody is A polyclonal antibody from goat, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Mouse Serpine2 with immunogen: Expression system for standard: NSO, Immunogen sequence: S20-P397. The kit is analytically validated with ready to use reagents. To measure Mouse Serpine2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Serpine2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Serpine2 in the sample.

Background/Introduction: Glia-derived nexin is a protein that in humans is encoded by the SERPINE2 gene. This gene is mapped to 1 C4; 1 40.97 cM. SERPINE2 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 386 aminoacids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).SERPINE2 is expressed with a 8 aminoacid His tag atCTerminus and purified by proprietary chromatographic techniques. This gene displays neurite promoting activity and serine protease inhibitory activity.

Small volumes of SERPINE2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS1751667 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the SerpinE2, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SERPINE2. The ELISA analytical biochemical technique of the MBS1751667 kit is based on SERPINE2 antibody-SERPINE2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SERPINE2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SERPINE2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).