Thy1 Cell Surface Antigen (THY1) ELISA Kit for Mouse

Name: Mouse THY1 ELISA Kit; Thy1 Cell Surface Antigen; MyBioSource
Brand: MyBioSource
SKU: MBS2021759
Price: 420 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2021759

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thy1 Cell Surface Antigen (Thy1). Standards or samples are then added to the appropriate… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Thy1 Cell Surface Antigen (Thy1), ELISA Kit

Full Product Name

Mouse Thy1 Cell Surface Antigen (Thy1) ELISA Kit

Product Gene Name

Thy1 elisa kit

Product Synonym Names

CD90; CDw90; THY mocyte differentiation antigen 1

Species Reactivity

Mouse

Assay Type

Double-antibody Sandwich

Samples

Serum, Plasma, Urine, Tissue homogenates, Cell lysates and other biological fluids

Detection Range

12.50-800pg/mL

Sensitivity

5.7pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of Thy1 Cell Surface Antigen (Thy1).
No significant cross-reactivity or interference between Thy1 Cell Surface Antigen (Thy1) and analogues was observed.

Preparation and Storage

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Molecular Weight

17,935 Da

OMIM

188230

3D Structure

ModBase 3D Structure for P04216

Supply Chain Verification

Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.

Application

Enzyme-linked immunosorbent assay for Antigen Detection.

Intra-assay Precision (Precision within an assay)

3 samples with low, middle and high level Thy1 Cell Surface Antigen (Thy1) were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays)

3 samples with low, middle and high level Thy1 Cell Surface Antigen (Thy1) were tested on 3 different plates, 8 replicates in each plate.

CV(%) =

SD/meanX100

Intra-Assay

CV<10%

Inter-Assay

CV<12%

Product Categories/Family for Thy1 elisa kit

CD & Adhesion molecule; Tumor immunity; Hematology; Neuroscience

ISO Certification

Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of Thy1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Thy1 purchase

MBS2021759 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Thy1 Cell Surface Antigen (Thy1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Thy1. The ELISA analytical biochemical technique of the MBS2021759 kit is based on Thy1 antibody-Thy1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Thy1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Thy1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for Thy1. It may not necessarily be applicable to this product.

NCBI GI #

119587894

NCBI GeneID

7070

NCBI Accession #

EAW67490.1

UniProt Primary Accession #

P04216

UniProt Secondary Accession #

Q16008; Q9NSP1

UniProt Related Accession #

P04216

NCBI Official Full Name

Thy-1 cell surface antigen, isoform CRA_a

NCBI Official Synonym Full Names

Thy-1 cell surface antigen

NCBI Official Symbol

THY1

NCBI Official Synonym Symbols

CD90; CDw90

UniProt Protein Name

Thy-1 membrane glycoprotein

UniProt Synonym Protein Names

CDw90; Thy-1 antigen; CD_antigen: CD90

Protein Family

Thy-1 membrane glycoprotein

UniProt Gene Name

THY1

UniProt Entry Name

THY1_HUMAN

NCBI Protein Information

thy-1 membrane glycoprotein

NCBI Summary for Thy1

This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

UniProt Comments for Thy1

THY1: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: anchored to external side of plasma membrane; focal adhesion; growth cone; endoplasmic reticulum; integral to plasma membrane; apical plasma membrane; dendrite; plasma membrane; cytosol; external side of plasma membrane; lipid raft

Molecular Function: integrin binding; protein binding; protein kinase binding

Biological Process: focal adhesion formation; cell-cell adhesion; retinal cone cell development; negative regulation of protein kinase activity; negative regulation of T cell receptor signaling pathway; cytoskeleton organization and biogenesis; negative regulation of axonogenesis; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of T cell activation; angiogenesis; T cell receptor signaling pathway; negative regulation of cell migration; positive regulation of GTPase activity

Research Articles on Thy1

1. CD133, CD44, and CD90 could be used as prognostic and predictive markers in hepatoblastoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with Thy1 elisa kit

Reference Product	PubMed Publications
ITGAM elisa kit	>15 publications with Thy1 and ITGAM
ITGB2 elisa kit	>3 publications with Thy1 and ITGB2
MAPK1 elisa kit	>2 publications with Thy1 and MAPK1
STAT6 elisa kit	>1 publications with Thy1 and STAT6

Pathways associated with Thy1 elisa kit

Products by Pathway	Pathway Diagram
Cardiac Progenitor Differentiation Pathway antibodies	Cardiac Progenitor Differentiation Pathway Diagram
IL4-mediated Signaling Events Pathway antibodies	IL4-mediated Signaling Events Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram
Amb2 Integrin Signaling Pathway antibodies	Amb2 Integrin Signaling Pathway Diagram

Diseases associated with Thy1 elisa kit

Disease Name	Pubmed Publications
Disease Models, Animal Antibodies	>225 publications with Thy1 and Disease Models, Animal
Kidney Diseases Antibodies	>194 publications with Thy1 and Kidney Diseases
Nervous System Diseases Antibodies	>147 publications with Thy1 and Nervous System Diseases
Brain Diseases Antibodies	>93 publications with Thy1 and Brain Diseases
Inflammation Antibodies	>79 publications with Thy1 and Inflammation
Neoplasms, Experimental Antibodies	>66 publications with Thy1 and Neoplasms, Experimental
Fibrosis Antibodies	>41 publications with Thy1 and Fibrosis
Necrosis Antibodies	>39 publications with Thy1 and Necrosis
Nerve Degeneration Antibodies	>34 publications with Thy1 and Nerve Degeneration
Liver Diseases Antibodies	>34 publications with Thy1 and Liver Diseases

Organs/Tissues associated with Thy1 elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>665 publications with Thy1 and Blood
Spleen Antibodies	>279 publications with Thy1 and Spleen
Bone Antibodies	>215 publications with Thy1 and Bone
Kidney Antibodies	>212 publications with Thy1 and Kidney
Bone Marrow Antibodies	>203 publications with Thy1 and Bone Marrow
Brain Antibodies	>191 publications with Thy1 and Brain
Nerve Antibodies	>141 publications with Thy1 and Nerve
Thymus Antibodies	>133 publications with Thy1 and Thymus
Liver Antibodies	>107 publications with Thy1 and Liver
Eye Antibodies	>106 publications with Thy1 and Eye