Maturation Promoting Factor (MPF) ELISA Kit for Mouse

Name: Mouse MPF ELISA Kit; Maturation Promoting Factor; MyBioSource
Brand: MyBioSource
SKU: MBS040904
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS040904

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of MPF (hereafter termed "analyte") in undiluted original… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Maturation Promoting Factor (MPF), ELISA Kit

Full Product Name

Mouse Maturation Promoting Factor ELISA Kit

Product Gene Name

MPF elisa kit

Species Reactivity

Mouse

Assay Type

Quantitative Sandwich

Detection Range

0.25nmol/L-8nmol/L

Sensitivity

0.1nmol/L

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

68,986 Da

OMIM

601051

3D Structure

ModBase 3D Structure for Q13421

Other Notes

Small volumes of MPF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MPF purchase

MBS040904 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Maturation Promoting Factor (MPF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MPF. The ELISA analytical biochemical technique of the MBS040904 kit is based on MPF antibody-MPF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MPF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MPF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MPF. It may not necessarily be applicable to this product.

NCBI GI #

53988378

NCBI GeneID

10232

NCBI Accession #

NP_005814.2

UniProt Primary Accession #

Q13421

UniProt Secondary Accession #

Q14859; Q4VQD5; Q96GR6; Q96KJ5; Q9BR17; Q9BTR2; Q9UCB2; Q9UK57; D3DU65

UniProt Related Accession #

Q13421

GenBank Nucleotide #

NM_005823.5

NCBI Official Full Name

mesothelin isoform 1 preproprotein

NCBI Official Synonym Full Names

mesothelin

NCBI Official Symbol

MSLN

NCBI Official Synonym Symbols

MPF; SMRP

UniProt Protein Name

Mesothelin

UniProt Synonym Protein Names

CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor

UniProt Gene Name

MSLN

UniProt Synonym Gene Names

MPF; MPF

UniProt Entry Name

MSLN_HUMAN

NCBI Protein Information

mesothelin; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor

NCBI Summary for MPF

This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]

UniProt Comments for MPF

MSLN: Membrane-anchored forms may play a role in cellular adhesion. Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer. Belongs to the mesothelin family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Cell adhesion

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: Golgi apparatus; extracellular space; cell surface; membrane; plasma membrane

Molecular Function: protein binding

Biological Process: pancreas development; cell adhesion

Research Articles on MPF

1. Use of S100P and mesothelin in cytologically borderline cases can increase the diagnostic accuracy for pancreatic adenocarcinoma.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with MPF elisa kit

Disease Name	Pubmed Publications
Carcinoma Antibodies	>17 publications with MPF and Carcinoma
Mesothelioma Antibodies	>17 publications with MPF and Mesothelioma
Pancreatic Neoplasms Antibodies	>16 publications with MPF and Pancreatic Neoplasms
Adenocarcinoma Antibodies	>15 publications with MPF and Adenocarcinoma
Neoplasms, Mesothelial Antibodies	>12 publications with MPF and Neoplasms, Mesothelial
Ovarian Neoplasms Antibodies	>10 publications with MPF and Ovarian Neoplasms
Carcinoma, Pancreatic Ductal Antibodies	>6 publications with MPF and Carcinoma, Pancreatic Ductal
Lung Diseases Antibodies	>6 publications with MPF and Lung Diseases
Neoplasms, Experimental Antibodies	>6 publications with MPF and Neoplasms, Experimental
Lung Neoplasms Antibodies	>5 publications with MPF and Lung Neoplasms

Organs/Tissues associated with MPF elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>11 publications with MPF and Lung
Liver Antibodies	>5 publications with MPF and Liver
Pancreas Antibodies	>4 publications with MPF and Pancreas
Ovary Antibodies	>3 publications with MPF and Ovary
Ascites Antibodies	>2 publications with MPF and Ascites
Stomach Antibodies	>2 publications with MPF and Stomach
Brain Antibodies	>1 publications with MPF and Brain
Intestine Antibodies	>1 publications with MPF and Intestine
Prostate Antibodies	>1 publications with MPF and Prostate
Cervix Antibodies	>1 publications with MPF and Cervix