Melatonin (MT) ELISA Kit for Goat

Name: Goat MT ELISA Kit; Melatonin; MyBioSource
Brand: MyBioSource
SKU: MBS285150
Price: 545 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS285150

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MT in samples. An antibody specific for MT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT present is bound by the… More details

$545 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Melatonin (MT), ELISA Kit

Full Product Name

Goat Melatonin (MT) ELISA Kit

Product Gene Name

MT elisa kit

Species Reactivity

Goat

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Goat MT. No significant cross-reactivity or interference between Goat MT and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

39,375 Da

OMIM

600665

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of MT elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for MT purchase

MBS285150 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Melatonin (MT) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MT. The ELISA analytical biochemical technique of the MBS285150 kit is based on MT antibody-MT antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MT antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MT. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for MT. It may not necessarily be applicable to this product.

NCBI GI #

5174593

NCBI GeneID

4543

NCBI Accession #

NP_005949.1

UniProt Secondary Accession #

A0AVC5; B0M0L2

UniProt Related Accession #

P48039

GenBank Nucleotide #

NM_005958.4

NCBI Official Full Name

melatonin receptor type 1A

NCBI Official Synonym Full Names

melatonin receptor 1A

NCBI Official Symbol

MTNR1A

NCBI Official Synonym Symbols

MT1; MEL-1A-R

UniProt Protein Name

Melatonin receptor type 1A

Protein Family

Melatonin receptor

UniProt Gene Name

MTNR1A

UniProt Synonym Gene Names

Mel-1A-R; Mel1a receptor

UniProt Entry Name

MTR1A_HUMAN

NCBI Protein Information

melatonin receptor type 1A

NCBI Summary for MT

This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. [provided by RefSeq, Jul 2008]

UniProt Comments for MT

MTNR1A: High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: cell soma; integral to plasma membrane; plasma membrane; receptor complex

Molecular Function: hormone binding; melatonin receptor activity; protein binding

Biological Process: aging; camera-type eye development; cell surface receptor linked signal transduction; circadian rhythm; G-protein signaling, coupled to cyclic nucleotide second messenger; mating behavior; negative regulation of insulin secretion; positive regulation of cGMP biosynthetic process

Research Articles on MT

1. There were no significant differences between the expression of MT1 and histological type, staging, grading, presence of residual disease, or overall survival time.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with MT elisa kit

Reference Product	PubMed Publications
MTNR1B elisa kit	>28 publications with MT and MTNR1B

Pathways associated with MT elisa kit

Products by Pathway	Pathway Diagram
Circadian Entrainment Pathway antibodies	Circadian Entrainment Pathway Diagram
Circadian Entrainment Pathway antibodies	Circadian Entrainment Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
G Alpha (i) Signalling Events Pathway antibodies	G Alpha (i) Signalling Events Pathway Diagram
GPCR Downstream Signaling Pathway antibodies	GPCR Downstream Signaling Pathway Diagram
GPCR Ligand Binding Pathway antibodies	GPCR Ligand Binding Pathway Diagram
GPCRs, Class A Rhodopsin-like Pathway antibodies	GPCRs, Class A Rhodopsin-like Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Neuroactive Ligand-receptor Interaction Pathway antibodies	Neuroactive Ligand-receptor Interaction Pathway Diagram
Signal Transduction Pathway antibodies	Signal Transduction Pathway Diagram

Diseases associated with MT elisa kit

Disease Name	Pubmed Publications
Carcinoma Antibodies	>5 publications with MT and Carcinoma
Diabetes Mellitus Antibodies	>3 publications with MT and Diabetes Mellitus
Child Development Disorders, Pervasive Antibodies	>2 publications with MT and Child Development Disorders, Pervasive
Neurotoxicity Syndromes Antibodies	>1 publications with MT and Neurotoxicity Syndromes
Brain Diseases Antibodies	>1 publications with MT and Brain Diseases
Hyperglycemia Antibodies	>1 publications with MT and Hyperglycemia
Immune System Diseases Antibodies	>1 publications with MT and Immune System Diseases
Drug-Related Side Effects and Adverse Reactions Antibodies	>1 publications with MT and Drug-Related Side Effects and Adverse Reactions
Kidney Diseases Antibodies	>1 publications with MT and Kidney Diseases
Necrosis Antibodies	>1 publications with MT and Necrosis

Organs/Tissues associated with MT elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>1 publications with MT and Liver