Nerve Growth Factor (NGF) ELISA Kit for Human

Name: Human NGF ELISA Kit; Nerve Growth Factor; MyBioSource
Brand: MyBioSource
SKU: MBS283264
Price: 470 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS283264

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NGF in samples. An antibody specific for NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNGF present is bound by the… More details

$470 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Nerve growth factor (NGF), ELISA Kit

Full Product Name

Human Nerve growth factor (NGF) ELISA Kit

Product Gene Name

NGF elisa kit

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Other biological fluids

Specificity

This assay has high sensitivity and excellent specificity for detection of Human NGF. No significant cross-reactivity or interference between Human NGF and analogues was observed.

Preparation and Storage

Store at 2-8 degree C.

Molecular Weight

26,959 Da

OMIM

V01511 Genomic DNA

3D Structure

ModBase 3D Structure for P01138

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Other Notes

Small volumes of NGF elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NGF purchase

MBS283264 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Nerve growth factor (NGF) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing NGF. The ELISA analytical biochemical technique of the MBS283264 kit is based on NGF antibody-NGF antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect NGF antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, NGF. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for NGF. It may not necessarily be applicable to this product.

NCBI GI #

70995319

NCBI GeneID

4803

NCBI Accession #

NP_002497.2

UniProt Primary Accession #

P01138

UniProt Secondary Accession #

Q6FHA0; Q96P60; Q9P2Q8; Q9UKL8; A1A4E5

UniProt Related Accession #

P01138

GenBank Nucleotide #

NM_002506.2

NCBI Official Full Name

beta-nerve growth factor

NCBI Official Synonym Full Names

nerve growth factor

NCBI Official Symbol

NGF

NCBI Official Synonym Symbols

NGFB; HSAN5; Beta-NGF

UniProt Protein Name

Beta-nerve growth factor

Protein Family

Beta-nerve growth factor

UniProt Gene Name

NGF

UniProt Synonym Gene Names

NGFB; Beta-NGF

NCBI Protein Information

beta-nerve growth factor

NCBI Summary for NGF

This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]

UniProt Comments for NGF

NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p13.2

Cellular Component: cytoplasmic vesicle; endosome; extracellular region; Golgi lumen

Molecular Function: growth factor activity; metalloendopeptidase inhibitor activity; nerve growth factor receptor binding; protein binding

Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; activation of MAPKK activity; cell-cell signaling; induction of apoptosis via death domain receptors; microtubule-based movement; negative regulation of apoptosis; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; negative regulation of neuron apoptosis; nerve growth factor processing; nerve growth factor receptor signaling pathway; neuron projection morphogenesis; phosphoinositide-mediated signaling; positive regulation of apoptosis; positive regulation of axonogenesis; positive regulation of Ras protein signal transduction; regulation of caspase activity; regulation of neuron differentiation; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Neuropathy, Hereditary Sensory And Autonomic, Type V

Research Articles on NGF

1. Many studies indicate that the only presence of NGF is unable to generate cell carcinogenesis, both in normal neuronal and non-neuronal cells/tissues. However, it cannot be excluded the possibility that the co-expression of NGF and pro-carcinogenic molecules might open to different consequence. [review]

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with NGF elisa kit

Reference Product	PubMed Publications
NTRK1 elisa kit	>2031 publications with NGF and NTRK1
NGFR elisa kit	>241 publications with NGF and NGFR
CASP3 elisa kit	>178 publications with NGF and CASP3
TRPV1 elisa kit	>95 publications with NGF and TRPV1
SHC1 elisa kit	>26 publications with NGF and SHC1
PLG elisa kit	>7 publications with NGF and PLG
GIPC1 elisa kit	>2 publications with NGF and GIPC1
SORCS3 elisa kit	>1 publications with NGF and SORCS3
MMP7 elisa kit	>1 publications with NGF and MMP7
SORT1 elisa kit	>1 publications with NGF and SORT1

Pathways associated with NGF elisa kit

Products by Pathway	Pathway Diagram
ARMS-mediated Activation Pathway antibodies	ARMS-mediated Activation Pathway Diagram
Activation Of TRKA Receptors Pathway antibodies	Activation Of TRKA Receptors Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Apoptosis Pathway antibodies	Apoptosis Pathway Diagram
Axonal Growth Stimulation Pathway antibodies	Axonal Growth Stimulation Pathway Diagram
BDNF Signaling Pathway antibodies	BDNF Signaling Pathway Diagram
Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies	Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
Ceramide Signalling Pathway antibodies	Ceramide Signalling Pathway Diagram
Frs2-mediated Activation Pathway antibodies	Frs2-mediated Activation Pathway Diagram
Id Signaling Pathway antibodies	Id Signaling Pathway Diagram

Diseases associated with NGF elisa kit

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>2216 publications with NGF and Nervous System Diseases
Pain Antibodies	>763 publications with NGF and Pain
Neurodegenerative Diseases Antibodies	>736 publications with NGF and Neurodegenerative Diseases
Inflammation Antibodies	>699 publications with NGF and Inflammation
Nerve Degeneration Antibodies	>611 publications with NGF and Nerve Degeneration
Peripheral Nervous System Diseases Antibodies	>466 publications with NGF and Peripheral Nervous System Diseases
Necrosis Antibodies	>447 publications with NGF and Necrosis
Hyperalgesia Antibodies	>318 publications with NGF and Hyperalgesia
Heart Diseases Antibodies	>126 publications with NGF and Heart Diseases
Memory Disorders Antibodies	>113 publications with NGF and Memory Disorders

Organs/Tissues associated with NGF elisa kit

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>660 publications with NGF and Muscle
Embryonic Tissue Antibodies	>613 publications with NGF and Embryonic Tissue
Skin Antibodies	>532 publications with NGF and Skin
Eye Antibodies	>432 publications with NGF and Eye
Lung Antibodies	>175 publications with NGF and Lung
Intestine Antibodies	>113 publications with NGF and Intestine
Spleen Antibodies	>92 publications with NGF and Spleen
Uncharacterized Tissue Antibodies	>1 publications with NGF and Uncharacterized Tissue