Occludin (OCLN) ELISA Kit for Rat

Name: Rat OCLN ELISA Kit; Occludin; MyBioSource
Brand: MyBioSource
SKU: MBS025670
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS025670

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of OCLN (hereafter termed "analyte") in undiluted original… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Occludin (OCLN), ELISA Kit

Full Product Name

Rat Occludin ELISA Kit

Product Gene Name

OCLN elisa kit

Species Reactivity

Rat

Assay Type

Quantitative Sandwich

Detection Range

31.2ng/ml-1000ng/ml

Sensitivity

5.0ng/ml

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

59,144 Da

Chromosome Location

Chromosome: 5; NC_000005.9 (68788119..68853931). Location: 5q13.1

OMIM

251290

3D Structure

ModBase 3D Structure for Q16625

Other Notes

Small volumes of OCLN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for OCLN purchase

MBS025670 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Occludin (OCLN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing OCLN. The ELISA analytical biochemical technique of the MBS025670 kit is based on OCLN antibody-OCLN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect OCLN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, OCLN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for OCLN. It may not necessarily be applicable to this product.

NCBI GI #

327478416

NCBI GeneID

100506658

NCBI Accession #

NP_001192184.1

UniProt Primary Accession #

Q16625

UniProt Secondary Accession #

Q5U1V4; Q8N6K1; B5BU70; D2DU64; D2DU65; D2IGC0; D2IGC1; E2CYV9

UniProt Related Accession #

Q16625

GenBank Nucleotide #

NM_001205255.1

NCBI Official Full Name

occludin isoform b

NCBI Official Synonym Full Names

occludin

NCBI Official Symbol

OCLN

NCBI Official Synonym Symbols

BLCPMG

UniProt Protein Name

Occludin

Protein Family

Occludin

UniProt Gene Name

OCLN

UniProt Entry Name

OCLN_HUMAN

NCBI Protein Information

occludin; tight junction protein occludin

NCBI Summary for OCLN

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]

UniProt Comments for OCLN

occludin: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. Interacts with TJP1/ZO1 and with VAPA. Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis. Belongs to the ELL/occludin family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q13.1

Cellular Component: tight junction; apicolateral plasma membrane; endocytic vesicle; apical plasma membrane; integral to membrane; plasma membrane; intercellular junction; cytoplasmic vesicle; cytosol; cell junction

Molecular Function: thiopurine S-methyltransferase activity; protein domain specific binding; protein binding; structural molecule activity

Biological Process: methylation; intercellular junction assembly and maintenance; apoptosis; S-adenosylmethionine metabolic process; protein complex assembly; S-adenosylhomocysteine metabolic process; cell structure disassembly during apoptosis

Disease: Band-like Calcification With Simplified Gyration And Polymicrogyria

Research Articles on OCLN

1. Loss of occludin is involved in adhesion, apoptosis, differentiation, Ca2+-homeostasis and neoplastic transformation in UV-exposed human keratinocytes and in skin neoplasms.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with OCLN elisa kit

Reference Product	PubMed Publications
TJP1 elisa kit	>503 publications with OCLN and TJP1
CLDN1 elisa kit	>259 publications with OCLN and CLDN1
CLDN5 elisa kit	>124 publications with OCLN and CLDN5
CLDN4 elisa kit	>61 publications with OCLN and CLDN4
TJP2 elisa kit	>48 publications with OCLN and TJP2
CLDN3 elisa kit	>47 publications with OCLN and CLDN3
CLDN7 elisa kit	>16 publications with OCLN and CLDN7
TJP3 elisa kit	>12 publications with OCLN and TJP3
CGN elisa kit	>7 publications with OCLN and CGN
YES1 elisa kit	>1 publications with OCLN and YES1

Pathways associated with OCLN elisa kit

Products by Pathway	Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Hepatitis C Pathway antibodies	Hepatitis C Pathway Diagram
Hepatitis C Pathway antibodies	Hepatitis C Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram
Leukocyte Transendothelial Migration Pathway antibodies	Leukocyte Transendothelial Migration Pathway Diagram
Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram
Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram
Pathogenic Escherichia Coli Infection Pathway antibodies	Pathogenic Escherichia Coli Infection Pathway Diagram
TGF-beta Receptor Signaling Pathway antibodies	TGF-beta Receptor Signaling Pathway Diagram

Diseases associated with OCLN elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>132 publications with OCLN and Inflammation
Nervous System Diseases Antibodies	>130 publications with OCLN and Nervous System Diseases
Disease Models, Animal Antibodies	>106 publications with OCLN and Disease Models, Animal
Neoplasms Antibodies	>98 publications with OCLN and Neoplasms
Necrosis Antibodies	>77 publications with OCLN and Necrosis
Cardiovascular Diseases Antibodies	>72 publications with OCLN and Cardiovascular Diseases
Edema Antibodies	>58 publications with OCLN and Edema
Drug Toxicity Antibodies	>50 publications with OCLN and Drug Toxicity
Hepatitis Antibodies	>34 publications with OCLN and Hepatitis
Liver Diseases Antibodies	>34 publications with OCLN and Liver Diseases

Organs/Tissues associated with OCLN elisa kit

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>255 publications with OCLN and Brain
Intestine Antibodies	>208 publications with OCLN and Intestine
Liver Antibodies	>112 publications with OCLN and Liver
Eye Antibodies	>94 publications with OCLN and Eye
Kidney Antibodies	>65 publications with OCLN and Kidney
Testis Antibodies	>54 publications with OCLN and Testis
Embryonic Tissue Antibodies	>49 publications with OCLN and Embryonic Tissue
Lung Antibodies	>44 publications with OCLN and Lung
Stomach Antibodies	>17 publications with OCLN and Stomach
Pancreas Antibodies	>14 publications with OCLN and Pancreas