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Osteonectin (ON) Recombinant Protein

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2029039
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MBS2029039 is a recombinant protein targeting ON. Also known as Recombinant Osteonectin (ON). Host: E ColiSource: Prokaryotic expression. More details

$215 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Osteonectin, Recombinant Protein
Full Product Name
Recombinant Osteonectin (ON)
Product Gene Name
ON recombinant protein
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGS- APQ QEALPDETEV VEETVAEVAE VAEVPVGANP VQVEVGEFEE VEETEEEVVA ENPCQNHHCK HGKVCELDEN NTPMCVCQDP TSCPAPVGEF EKVCSNDNKT FDSSCHFFAT KCTLEGTKKG HKLHLDYIGP CKYIPPCLDS ELSEFPLRMR DWLKNVLVTL YERDEGNNLL TEKQKLRVKK IHENEKRLEA GDHPVELLAR DFEKNYNMYI FPVHWQFGQL DQHPIDGYLS HTELAPLRAP LIPMEHCTTR FFE
Host
Host: E Coli
Source: Prokaryotic expression
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Purity/Purification
>95%
Concentration
5.2 (lot specific)
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Molecular Weight
34,632 Da
OMIM
182120
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Predicted Molecular Mass
32.8kDa
Accurate Molecular Mass (KD)
40kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and T7-tag
Organism Species
Oryctolagus cuniculus (Rabbit)
Fragment
Ala18~Glu273
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for ON recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

SDS-Page of ON recombinant protein

ON Recombinant Protein – Cat# MBS2029039

Other Notes

Small volumes of ON recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ON. It may not necessarily be applicable to this product.

NCBI GI #
338313
NCBI GeneID
6678
NCBI Accession #
AAA60570.1
UniProt Secondary Accession #
Q6IBK4; D3DQH9
UniProt Related Accession #
P09486
NCBI Official Full Name
osteonectin
NCBI Official Synonym Full Names
secreted protein, acidic, cysteine-rich (osteonectin)
NCBI Official Symbol
SPARC
NCBI Official Synonym Symbols
ON; BM-40
UniProt Protein Name
SPARC
UniProt Synonym Protein Names
Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine
Protein Family
SPARC
UniProt Gene Name
SPARC
UniProt Synonym Gene Names
ON; BM-40; ON
UniProt Entry Name
SPRC_HUMAN
NCBI Protein Information
SPARC

NCBI Summary for ON

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]
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UniProt Comments for ON

SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q31.3-q32

Cellular Component: extracellular space; platelet alpha granule membrane; nuclear matrix; cell surface; mitochondrion; cytoplasm; plasma membrane; extracellular region; basement membrane

Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding

Biological Process: platelet activation; response to gravity; receptor-mediated endocytosis; response to peptide hormone stimulus; ossification; extracellular matrix organization and biogenesis; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; response to ethanol; platelet degranulation; response to cadmium ion; response to cytokine stimulus; negative regulation of endothelial cell proliferation; response to lead ion; regulation of cell morphogenesis; response to calcium ion; blood coagulation; inner ear development; lung development

Disease: Osteogenesis Imperfecta, Type Xvii
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Research Articles on ON

1. SCD5 impairs SPARC and cathepsin B secretion in human melanoma cells and intracellular pH acidification.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ON recombinant protein

 Reference Product  PubMed Publications
 TGFB1 recombinant protein  >72 publications with ON and TGFB1
 COL1A1 recombinant protein  >35 publications with ON and COL1A1
 COL3A1 recombinant protein  >24 publications with ON and COL3A1
 FN1 recombinant protein  >16 publications with ON and FN1
 TIMP1 recombinant protein  >8 publications with ON and TIMP1
 VEGFA recombinant protein  >8 publications with ON and VEGFA
 VWF recombinant protein  >7 publications with ON and VWF
 PLG recombinant protein  >2 publications with ON and PLG
 ALB recombinant protein  >1 publications with ON and ALB

Pathways associated with ON recombinant protein

 Products by Pathway  Pathway Diagram
 Binding And Uptake Of Ligands By Scavenger Receptors Pathway antibodies  Binding And Uptake Of Ligands By Scavenger Receptors Pathway Diagram
 ECM Proteoglycans Pathway antibodies  ECM Proteoglycans Pathway Diagram
 Extracellular Matrix Organization Pathway antibodies  Extracellular Matrix Organization Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
 Scavenging By Class H Receptors Pathway antibodies  Scavenging By Class H Receptors Pathway Diagram
 Senescence And Autophagy Pathway antibodies  Senescence And Autophagy Pathway Diagram
 TGF-beta Receptor Signaling Pathway antibodies  TGF-beta Receptor Signaling Pathway Diagram

Diseases associated with ON recombinant protein

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >108 publications with ON and Inflammation
 Disease Models, Animal Antibodies  >107 publications with ON and Disease Models, Animal
 Fibrosis Antibodies  >100 publications with ON and Fibrosis
 Breast Neoplasms Antibodies  >81 publications with ON and Breast Neoplasms
 Brain Diseases Antibodies  >77 publications with ON and Brain Diseases
 Necrosis Antibodies  >65 publications with ON and Necrosis
 Neoplasms, Experimental Antibodies  >54 publications with ON and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >52 publications with ON and Lung Neoplasms
 Lung Diseases Antibodies  >51 publications with ON and Lung Diseases
 Kidney Diseases Antibodies  >46 publications with ON and Kidney Diseases

Organs/Tissues associated with ON recombinant protein

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >1110 publications with ON and Bone
 Connective Tissue Antibodies  >454 publications with ON and Connective Tissue
 Blood Antibodies  >341 publications with ON and Blood
 Vascular Antibodies  >310 publications with ON and Vascular
 Bone Marrow Antibodies  >208 publications with ON and Bone Marrow
 Muscle Antibodies  >193 publications with ON and Muscle
 Embryonic Tissue Antibodies  >177 publications with ON and Embryonic Tissue
 Mouth Antibodies  >163 publications with ON and Mouth
 Brain Antibodies  >153 publications with ON and Brain
 Eye Antibodies  >124 publications with ON and Eye
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