Akt, Phosphorylated (Ser473) (Rac Pka, Pkba) Blocking Peptide – Synthetic Peptide

Function: Serine/threonine kinase involved in various developmental processes. During early embryogenesis, acts as a survival protein. During mid-embryogenesis, phosphorylates and activates trh, a transcription factor required for tracheal cell fate determination. Also regulates tracheal cell migration. Later in development, acts downstream of PI3K and Pk61C/PDK1 in the insulin receptor transduction pathway which regulates cell growth and organ size, by phosphorylating and antagonizing FOXO transcription factor. Controls follicle cell size during oogenesis. May also stimulate cell growth by phosphorylating Gig/Tsc2 and inactivating the Tsc complex. Dephosphorylation of 'Ser-586' by Phlpp triggers apoptosis and suppression of tumor growth. Ref.6 Ref.7 Ref.8 Ref.9 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15 Ref.16 Ref.17

Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Ref.2

Subcellular location: Cytoplasm › cytosol. Cell membrane. Note: Recruited to plasma membrane upon activation.

Tissue specificity: Ubiquitously expressed. Present in ovary, where it is concentrated at the basal side of follicle cells. Ref.2

Developmental stage: Expressed both maternally and zygotically. Strongly expressed in embryo and pupa. Weakly expressed in larva. Mildly expressed in adult. Ref.2 Ref.17

Domain: Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI3K) results in its targeting to the plasma membrane

Probable.

Post-translational modification: Phosphorylated and activated by Pk61C/PDK1. Phosphorylated on Ser-586 by the rictor-Tor complex. Ref.8 Ref.10 Ref.18 Ref.19

Disruption phenotype: Death at the first instar larval stage. Ref.6

Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 PH domain.Contains 1 protein kinase domain.