Platelet Factor 4 (PF4) ELISA Kit for Sheep

Name: Sheep PF4 ELISA Kit; Platelet Factor 4; MyBioSource
Brand: MyBioSource
SKU: MBS740037
Price: 580 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS740037

Intended Uses: This PF4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep PF4. This ELISA kit for research use only, not for therapeutic or test applications!Principle of the Assay: PF4 ELISA kit applies the… More details

$580 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Platelet Factor 4 (PF4), ELISA Kit

Full Product Name

Sheep Platelet Factor 4 ELISA Kit

Product Gene Name

PF4 elisa kit

Species Reactivity

Sheep

Assay Type

Quantitative Competitive

Samples

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Sensitivity

1.0 pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of PF4. No significant cross-reactivity or interference between PF4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PF4 and all the analogues, therefore, cross reaction may still exist in some cases.

Preparation and Storage

Store all reagents at 2-8 degree C.

Molecular Weight

10,845 Da

OMIM

173460

Sample Preparation

We suggest pre-experimenting with neat (undiluted) samples, 1:2 or 1:4 dilutions. Please avoid diluting your samples more than 1:10 as it would exceed the dilution limit set for this kit. If the expected concentration of the target is beyond the detection range of the kit, please contact our technical support team

Product Categories/Family for PF4 elisa kit

Cardiovascular

ISO Certification

Manufactured in an ISO 13485:2016 Certified Laboratory.

Other Notes

Small volumes of PF4 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PF4 purchase

MBS740037 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Platelet Factor 4 (PF4) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PF4. The ELISA analytical biochemical technique of the MBS740037 kit is based on PF4 antibody-PF4 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PF4 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PF4. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PF4. It may not necessarily be applicable to this product.

NCBI GI #

4505733

NCBI GeneID

5196

NCBI Accession #

NP_002610.1

UniProt Secondary Accession #

Q53X61; Q9UC64; Q9UC65

UniProt Related Accession #

P02776

GenBank Nucleotide #

NM_002619.3

NCBI Official Full Name

platelet factor 4

NCBI Official Synonym Full Names

platelet factor 4

NCBI Official Symbol

PF4

NCBI Official Synonym Symbols

PF-4; CXCL4; SCYB4

UniProt Protein Name

Platelet factor 4

UniProt Synonym Protein Names

C-X-C motif chemokine 4; Iroplact; Oncostatin-ACleaved into the following chain:Platelet factor 4, short form

Protein Family

Platelet factor

UniProt Gene Name

PF4

UniProt Synonym Gene Names

CXCL4; SCYB4; PF-4

UniProt Entry Name

PLF4_HUMAN

NCBI Protein Information

platelet factor 4; iroplact; oncostatin-A; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4

NCBI Summary for PF4

This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. [provided by RefSeq, Mar 2012]

UniProt Comments for PF4

PF4: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Secreted, signal peptide; Apoptosis; Motility/polarity/chemotaxis; Chemokine

Chromosomal Location of Human Ortholog: 4q12-q21

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CXCR3 chemokine receptor binding; chemokine activity

Biological Process: negative regulation of cytolysis; platelet activation; cytokine and chemokine mediated signaling pathway; leukocyte chemotaxis; response to lipopolysaccharide; positive regulation of leukocyte chemotaxis; positive regulation of cAMP metabolic process; positive regulation of tumor necrosis factor production; negative regulation of megakaryocyte differentiation; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; platelet degranulation; negative regulation of MHC class II biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; immune response; blood coagulation; inflammatory response; positive regulation of macrophage differentiation

Research Articles on PF4

1. Plasma VEGF was significantly reduced in low birth weight (LBW) neonates while that of sVEGFR-1 and PF4 were significantly higher. VEGF/sVEGF/PF4 pathway seems to be involved in the endothelial progenitor cell dysfunction in LBW neonates.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PF4 elisa kit

Reference Product	PubMed Publications
FGF2 elisa kit	>16 publications with PF4 and FGF2
CXCR3 elisa kit	>14 publications with PF4 and CXCR3
PROC elisa kit	>12 publications with PF4 and PROC
CXCL11 elisa kit	>6 publications with PF4 and CXCL11

Pathways associated with PF4 elisa kit

Products by Pathway	Pathway Diagram
CXCR3-mediated Signaling Events Pathway antibodies	CXCR3-mediated Signaling Events Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Chemokine Receptors Bind Chemokines Pathway antibodies	Chemokine Receptors Bind Chemokines Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Chemokine Signaling Pathway antibodies	Chemokine Signaling Pathway Diagram
Class A/1 (Rhodopsin-like Receptors) Pathway antibodies	Class A/1 (Rhodopsin-like Receptors) Pathway Diagram
Common Pathway antibodies	Common Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies	Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram

Diseases associated with PF4 elisa kit

Disease Name	Pubmed Publications
Thrombocytopenia Antibodies	>467 publications with PF4 and Thrombocytopenia
Cardiovascular Diseases Antibodies	>367 publications with PF4 and Cardiovascular Diseases
Vascular Diseases Antibodies	>344 publications with PF4 and Vascular Diseases
Thrombosis Antibodies	>310 publications with PF4 and Thrombosis
Inflammation Antibodies	>76 publications with PF4 and Inflammation
Nervous System Diseases Antibodies	>67 publications with PF4 and Nervous System Diseases
Autoimmune Diseases Antibodies	>58 publications with PF4 and Autoimmune Diseases
Kidney Diseases Antibodies	>57 publications with PF4 and Kidney Diseases
Atherosclerosis Antibodies	>55 publications with PF4 and Atherosclerosis
Lung Diseases Antibodies	>44 publications with PF4 and Lung Diseases

Organs/Tissues associated with PF4 elisa kit

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>102 publications with PF4 and Heart
Bone Antibodies	>76 publications with PF4 and Bone
Bone Marrow Antibodies	>67 publications with PF4 and Bone Marrow
Skin Antibodies	>35 publications with PF4 and Skin
Lung Antibodies	>32 publications with PF4 and Lung
Embryonic Tissue Antibodies	>18 publications with PF4 and Embryonic Tissue
Intestine Antibodies	>7 publications with PF4 and Intestine
Placenta Antibodies	>1 publications with PF4 and Placenta