Phosphogluconate Dehydrogenase (PGD) Active Protein

Name: PGD Active Protein; MyBioSource
Brand: MyBioSource
SKU: MBS846979
Price: 225 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS846979

PGD (Phosphogluconate dehydrogenase); also known as 6PGD; is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

Phosphogluconate dehydrogenase (PGD), Active Protein

Full Product Name

Phosphogluconate dehydrogenase; human recombinant

Product Gene Name

PGD active protein

Product Synonym Names

6PGD; PGD

Sequence

MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA

Host

E Coli
Source: Human

Form/Format

Liquid
In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT; 0.1M Nacl; 10% glycerol

Purity/Purification

> 90%

Preparation and Storage

Store at -20 degree C for 12 months.
Centrifuge the vial prior to opening.

Molecular Weight

53.3 kDa

OMIM

U30255 mRNA

3D Structure

ModBase 3D Structure for P52209

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Biological Activity

Specific activity is >10 units/mg, in which one unit oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25C, in the presence of beta-NADP.

Results

>10 units/mg

Unit Definition

One unit is the amount of enzyme that oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25C; in the presence of beta-NADP.

Applications Tested/Suitable for PGD active protein

SDS-PAGE

Other Notes

Small volumes of PGD active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PGD purchase

MBS846979 is an active protein targeting PGD. Also known as Phosphogluconate dehydrogenase; human recombinant. Host: E Coli
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for PGD. It may not necessarily be applicable to this product.

NCBI GI #

751130505

NCBI GeneID

5226

NCBI Accession #

NP_001291380.1

UniProt Primary Accession #

P52209

UniProt Secondary Accession #

Q9BWD8; A8K2Y9; B4DQJ8

UniProt Related Accession #

P52209

GenBank Nucleotide #

NM_001304451.1

NCBI Official Full Name

6-phosphogluconate dehydrogenase, decarboxylating isoform 2

NCBI Official Synonym Full Names

phosphogluconate dehydrogenase

NCBI Official Symbol

PGD

NCBI Official Synonym Symbols

6PGD

UniProt Protein Name

6-phosphogluconate dehydrogenase, decarboxylating

Protein Family

Putative 6-phosphogluconate dehydrogenase

UniProt Gene Name

PGD

UniProt Synonym Gene Names

PGDH

NCBI Protein Information

6-phosphogluconate dehydrogenase, decarboxylating

NCBI Summary for PGD

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]

UniProt Comments for PGD

PGD: an oxidative carboxylase that catalyses the decarboxylating reduction of 6-phosphogluconate into ribulose 5-phosphate in the presence of NADP. This reaction is a component of the hexose mono-phosphate shunt and pentose phosphate pathways.

Protein type: Carbohydrate Metabolism - pentose phosphate pathway; EC 1.1.1.44; Other Amino Acids Metabolism - glutathione; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: cytosol; nucleus

Molecular Function: phosphogluconate dehydrogenase (decarboxylating) activity

Biological Process: pentose-phosphate shunt; pentose-phosphate shunt, oxidative branch

Research Articles on PGD

1. Studies indicate link between the 6-phosphogluconate dehydrogenase, oxidative pentose phosphate pathway (PPP) and lipogenesis through Ru-5-P-dependent inhibition of serine/threonine protein kinase LKB1-AMPK signalling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PGD active protein

Reference Product	PubMed Publications
PGM1 active protein	>146 publications with PGD and PGM1
GPI active protein	>62 publications with PGD and GPI
G6PD active protein	>59 publications with PGD and G6PD
RPE active protein	>4 publications with PGD and RPE
TKT active protein	>3 publications with PGD and TKT
TALDO1 active protein	>1 publications with PGD and TALDO1
RPIA active protein	>1 publications with PGD and RPIA
UBC active protein	>1 publications with PGD and UBC
H6PD active protein	>1 publications with PGD and H6PD

Pathways associated with PGD active protein

Products by Pathway	Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Carbon Metabolism Pathway antibodies	Carbon Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Metabolic Pathways antibodies	Metabolic Pathways Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram
Metabolism Of Carbohydrates Pathway antibodies	Metabolism Of Carbohydrates Pathway Diagram
Pentose Phosphate Pathway antibodies	Pentose Phosphate Pathway Diagram
Pentose Phosphate Pathway antibodies	Pentose Phosphate Pathway Diagram
Pentose Phosphate Pathway antibodies	Pentose Phosphate Pathway Diagram

Diseases associated with PGD active protein

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>272 publications with PGD and Nervous System Diseases
Inflammation Antibodies	>243 publications with PGD and Inflammation
Brain Diseases Antibodies	>124 publications with PGD and Brain Diseases
Infertility, Male Antibodies	>106 publications with PGD and Infertility, Male
Fibrosis Antibodies	>104 publications with PGD and Fibrosis
Anemia Antibodies	>102 publications with PGD and Anemia
Heart Diseases Antibodies	>55 publications with PGD and Heart Diseases
Neurobehavioral Manifestations Antibodies	>53 publications with PGD and Neurobehavioral Manifestations
Necrosis Antibodies	>53 publications with PGD and Necrosis
Kidney Diseases Antibodies	>44 publications with PGD and Kidney Diseases

Organs/Tissues associated with PGD active protein

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>747 publications with PGD and Blood
Lung Antibodies	>264 publications with PGD and Lung
Brain Antibodies	>181 publications with PGD and Brain
Heart Antibodies	>141 publications with PGD and Heart
Vascular Antibodies	>119 publications with PGD and Vascular
Bone Antibodies	>109 publications with PGD and Bone
Muscle Antibodies	>93 publications with PGD and Muscle
Bone Marrow Antibodies	>83 publications with PGD and Bone Marrow
Uterus Antibodies	>80 publications with PGD and Uterus
Skin Antibodies	>77 publications with PGD and Skin