Polymerase (DNA Directed), Lambda (POLL) ELISA Kit for Human

Name: Human POLL ELISA Kit; Polymerase (DNA Directed), Lambda; MyBioSource
Brand: MyBioSource
SKU: MBS9329278
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9329278

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of POLL (hereafter termed "analyte") in undiluted original… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

polymerase (DNA directed) , lambda (POLL), ELISA Kit

Full Product Name

Human DNA polymerase lambda, POLL ELISA Kit

Product Gene Name

POLL elisa kit

Product Synonym Names

Human DNA polymerase lambda (POLL) ELISA kit; RP11-529I10.1; BETAN; FLJ46002; POLKAPPA; DNA polymerase beta-2; DNA polymerase kappa (DNA polymerase beta-N) ; DNA polymerase lamda2; DNA-directed DNA polymerase lambda; OTTHUMP00000020323; pol beta2; pol; polymerase (DNA directed); lambda

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Detection Range

0.25ng/ml-8ng/ml

Sensitivity

0.1ng/ml

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

63,482 Da

OMIM

606343

3D Structure

ModBase 3D Structure for Q9UGP5

Other Notes

Small volumes of POLL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for POLL purchase

MBS9329278 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the polymerase (DNA directed), lambda (POLL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing POLL. The ELISA analytical biochemical technique of the MBS9329278 kit is based on POLL antibody-POLL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect POLL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, POLL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for POLL. It may not necessarily be applicable to this product.

NCBI GI #

291463287

NCBI GeneID

27343

NCBI Accession #

NP_001167555.1

UniProt Primary Accession #

Q9UGP5

UniProt Secondary Accession #

Q5JQP5; Q6NUM2; Q9HA10; Q9HB35; D3DR76

UniProt Related Accession #

Q9UGP5

GenBank Nucleotide #

NM_001174084.1

NCBI Official Full Name

DNA polymerase lambda isoform a

NCBI Official Synonym Full Names

polymerase (DNA directed), lambda

NCBI Official Symbol

POLL

NCBI Official Synonym Symbols

BETAN; POLKAPPA

UniProt Protein Name

DNA polymerase lambda

UniProt Synonym Protein Names

DNA polymerase beta-2; Pol beta2; DNA polymerase kappa

Protein Family

DNA polymerase

UniProt Gene Name

POLL

UniProt Synonym Gene Names

Pol Lambda; Pol beta2

UniProt Entry Name

DPOLL_HUMAN

NCBI Protein Information

DNA polymerase lambda; DNA polymerase kappa; DNA polymerase beta-2; DNA polymerase beta-N

NCBI Summary for POLL

This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010]

UniProt Comments for POLL

POLL: Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. Belongs to the DNA polymerase type-X family.

Protein type: Lyase; EC 2.7.7.7; DNA repair, damage; Transferase

Chromosomal Location of Human Ortholog: 10q23

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; lyase activity; DNA-directed DNA polymerase activity

Biological Process: nucleotide-excision repair; somatic hypermutation of immunoglobulin genes; DNA replication

Research Articles on POLL

1. A specific N-terminal extension of the 8 kDa domain of DNA polymerase lambda is important for the non-homologous end joining function.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with POLL elisa kit

Reference Product	PubMed Publications
PCNA elisa kit	>1 publications with POLL and PCNA

Pathways associated with POLL elisa kit

Products by Pathway	Pathway Diagram
BER Complex Pathway antibodies	BER Complex Pathway Diagram
BER Complex Pathway antibodies	BER Complex Pathway Diagram
Base Excision Repair Pathway antibodies	Base Excision Repair Pathway Diagram
Base Excision Repair Pathway antibodies	Base Excision Repair Pathway Diagram
Non-homologous End-joining Pathway antibodies	Non-homologous End-joining Pathway Diagram
Non-homologous End-joining Pathway antibodies	Non-homologous End-joining Pathway Diagram

Diseases associated with POLL elisa kit

Disease Name	Pubmed Publications
Inflammation Antibodies	>319 publications with POLL and Inflammation
Nervous System Diseases Antibodies	>193 publications with POLL and Nervous System Diseases
Lung Diseases Antibodies	>171 publications with POLL and Lung Diseases
Neoplasms Antibodies	>170 publications with POLL and Neoplasms
Necrosis Antibodies	>160 publications with POLL and Necrosis
Central Nervous System Diseases Antibodies	>126 publications with POLL and Central Nervous System Diseases
Skin Diseases Antibodies	>61 publications with POLL and Skin Diseases
Liver Diseases Antibodies	>58 publications with POLL and Liver Diseases
Heart Diseases Antibodies	>51 publications with POLL and Heart Diseases
Carcinoma Antibodies	>40 publications with POLL and Carcinoma

Organs/Tissues associated with POLL elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>510 publications with POLL and Blood
Lung Antibodies	>197 publications with POLL and Lung
Liver Antibodies	>124 publications with POLL and Liver
Brain Antibodies	>114 publications with POLL and Brain
Bone Antibodies	>86 publications with POLL and Bone
Vascular Antibodies	>80 publications with POLL and Vascular
Heart Antibodies	>64 publications with POLL and Heart
Kidney Antibodies	>62 publications with POLL and Kidney
Muscle Antibodies	>60 publications with POLL and Muscle
Skin Antibodies	>39 publications with POLL and Skin