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Beta Actin (ACTB) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS3901059

Cytoskeletal beta-actin is one of six highly conserved actin isoforms that is expressed ubiquitously in eukaryotic cells. Cytoskeletal beta-actin functions to maintain cell structure and plays a role in motility. More details

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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
beta Actin (ACTB), Polyclonal Antibody
Full Product Name
Rabbit anti-beta Actin Antibody, Affinity Purified
Product Gene Name
Product Synonym Names
Beta-actin; PS1TP5BP1; PS1TP5-binding protein 1; beta cytoskeletal actin; actin, cytoplasmic 1; I(2)-actin; BRWS1; Actin, cytoplasmic 1; actin beta
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Mouse
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Purity/Purification
Antigen Affinity Purified
Concentration
1000ug/ml (lot specific)
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Molecular Weight
375
Chromosome Location
Chromosome: 7; NC_000007.13 (5566779..5570232, complement). Location: 7p22
OMIM
102630
3D Structure
ModBase 3D Structure for P60709
Predicted Reactivity
Rat, X. tropicalis, Chicken, Sheep, Bovine, Dog, Horse, Rabbit, Pig, Golden hamster, Orangutan, Chimpanzee
Immunogen
Between 1 and 50
Conjugation
Unconjugated

Product Categories/Family for anti-ACTB antibody

Applications Tested/Suitable for anti-ACTB antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ACTB antibody
WB: 1:10,000-1:25,000
IHC: 1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Immunohistochemistry (IHC) of anti-ACTB antibody

Detection of human beta Actin by immunohistochemistry. Sample: FFPE section of human prostate cancer. Antibody: Affinity purified rabbit anti-beta Actin (MBS3901059 Lot 18) used at 1:1,000 (1ug/ml). Detection: DAB
Rabbit Anti-ACTB Polyclonal Antibody – Cat# MBS3901059

Western Blot (WB) of anti-ACTB antibody

Detection of human beta Actin by western blot. Samples: Whole cell lysate (10 ug) from U2OS, A-549, K-562, HeLa, HEK293T, Jurkat, MOLT-4, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-beta Actin antibody MBS3901059 Lot 18 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rabbit Anti-ACTB Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901059

Western Blot (WB) of anti-ACTB antibody

Detection of mouse beta Actin by western blot. Samples: Whole cell lysate (10 ug) from BW5147.3, mIMCD-3, A20, NIH 3T3, CT26, EL4, RenCa, CH27, C2C12, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-beta Actin antibody MBS3901059 Lot 18 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Rabbit Anti-ACTB Polyclonal Antibody, Western Blot (WB) – Cat# MBS3901059

Other Notes

Small volumes of anti-ACTB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACTB purchase

MBS3901059 is an antibody targeting ACTB. Also known as Rabbit anti-beta Actin Antibody, Affinity Purified. Reactive in Human, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ACTB. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
actin, cytoplasmic 1
NCBI Official Synonym Full Names
actin, beta
NCBI Official Symbol
ACTB
NCBI Official Synonym Symbols
BRWS1; PS1TP5BP1
UniProt Protein Name
Actin, cytoplasmic 1
UniProt Synonym Protein Names
Beta-actin
Protein Family
UniProt Gene Name
ACTB
UniProt Entry Name
ACTB_HUMAN
NCBI Protein Information
actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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