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anti-ASK1 antibody :: Rabbit ASK1 Polyclonal Antibody

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Catalog # MBS223333
Unit / Price
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  0.1 mg  /  $340 +1 FREE 8GB USB
Product Name

ASK1, Polyclonal Antibody

Full Product Name

RABBIT ANTI HUMAN ASK1 (C-TERMINAL)

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
602448
3D Structure
ModBase 3D Structure for Q99683
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Specificity
This item recognises human ASK1 (Apoptosis signal-regulating kinase 1) a 155kDa protein belonging to the protein kinase superfamily.

ASK1 is a serine/threonine kinase, also known as MAP kinase kinase kinase (MAPKKK), and plays an important role in the apoptotic response to various stimuli including oxidative stress, cytokines (e.g. TNF) and endoplasmic reticulum stress. Akt in conjunction to Hsp90 phosphorylates ASK1 at Ser83 to inhibit ASK1 induced apoptosis.

ASK1 is widely expressed in a variety of human tissues including the heart and pancreas.
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5 mg/ml (lot specific)
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to amino acids near the carboxy terminus of human ASK1.
Buffer Solution
Antiserum Preparation
Antiserum to human ASK1 (CT) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Other Notes
Small volumes of anti-ASK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-ASK1 antibody
MBS223333 recognises human ASK1 (Apoptosis signal-regulating kinase 1) a 155kDa protein belonging to the protein kinase superfamily. ASK1 is a serine/threonine kinase, also known as MAP kinase kinase kinase (MAPKKK), and plays an important role in the apoptotic response to various stimuli including oxidative stress, cytokines (e.g. TNF) and endoplasmic reticulum stress. Akt in conjunction to Hsp90 phosphorylates ASK1 at Ser83 to inhibit ASK1 induced apoptosis. ASK1 is widely expressed in a variety of human tissues including the heart and pancreas.
Applications Tested/Suitable for anti-ASK1 antibody
Immunohistology Frozen, Western Blot (WB)
Application Notes for anti-ASK1 antibody
Western Blot: This item detects a band of approximately 155kDa in SW1353 cell lysates.
Immunohistology - Frozen: Maximum Dilution: 10ug/ml
Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml

Testing Data #1 of anti-ASK1 antibody
Western Blot analysis of whole cell lysate from SW1353 chondrosarcoma cell line probed with Rabbit anti Human ASK1 (MBS223333)
anti-ASK1 antibody Testing Data #1 image
Testing Data #2 of anti-ASK1 antibody
Immunocytochemical staining of A431 epithelial carcinoma cell line with Rabbit anti Human ASK1 (MBS223333)
anti-ASK1 antibody Testing Data #2 image
NCBI/Uniprot data below describe general gene information for ASK1. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
69,839 Da
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 5
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 5
NCBI Official Symbol
MAP3K5  [Similar Products]
NCBI Official Synonym Symbols
ASK1; MEKK5; MAPKKK5
  [Similar Products]
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 5; ASK-1; MAP/ERK kinase kinase 5; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5; apoptosis signal regulating kinase 1; apoptosis signal-regulating kinase 1
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 5
UniProt Synonym Protein Names
Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5
Protein Family
UniProt Gene Name
MAP3K5  [Similar Products]
UniProt Synonym Gene Names
ASK1; MAPKKK5; MEKK5; ASK-1; MEK kinase 5; MEKK 5  [Similar Products]
UniProt Entry Name
M3K5_HUMAN
NCBI Summary for ASK1
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008]
UniProt Comments for ASK1
ASK1: a MAP kinase kinase kinase of the STE11 family, apoptosis signal-regulating kinase 1 (ASK1) plays essential roles in stress-induced apoptosis. Activated by various stressors, including oxidative stress, endoplasmic reticulum stress, and calcium overload, as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) and lipopolysaccharide (LPS). In turn, ASK1 phosphorylates and activates two different subgroups of MAP kinase kinases, MKK4/SEK1 and MKK3/MKK6, which in turn activate stress-activated protein kinases (JNKs) and p38 subgroups of MAP kinases, respectively. Overexpression activates JNK and p38 and induces apoptosis in several cell types through signals involving the mitochondrial cell death pathway. Embryonic fibroblasts or primary neurons derived from ASK1-/- mice are resistant to stress-induced JNK and p38 activation as well as cell death. Oxidative stress induces the activation of ASK1, which correlate with ASK1 activity and ASK1-dependent apoptosis. Required for the innate immune response, which is essential for host defense against a wide range of pathogens.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.25; Protein kinase, STE; STE group; STE11 family

Chromosomal Location of Human Ortholog: 6q22.33

Cellular Component: cytosol

Molecular Function: protein binding; protein homodimerization activity; MAP kinase kinase kinase activity; magnesium ion binding; caspase activator activity; protein phosphatase binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: caspase activation; positive regulation of JNK activity; viral reproduction; activation of MAPKK activity; positive regulation of apoptosis; MAPKKK cascade; positive regulation of JNK cascade; positive regulation of caspase activity; protein amino acid phosphorylation; activation of JNK activity; innate immune response; JNK cascade; induction of apoptosis by oxidative stress
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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