Rabbit Anti-CCNE1 Polyclonal Antibody – Cat# MBS440015

Cyclin E (CCNE1) Antibody – Rabbit Polyclonal

Name: CCNE1 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS440015
Price: 325 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS440015

During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks (1,2). Early in the G1 phase of the cell cycle cyclin D1 induction is followed by… More details

$325 / 0.2 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

cyclin E (Ccne1), Polyclonal Antibody

Full Product Name

cyclin E (C19) Antibody

Product Gene Name

anti-Ccne1 antibody

Clonality

Polyclonal

Host

Rabbit

Form/Format

Each vial contains 200 ug/ml of affinity purified rabbit IgG, cyclin E (C19) MBS440015, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.

Specificity

Cyclin E (C19) MBS440015 reacts with cyclin E of mouse, rat, and human origin

Preparation and Storage

Store this product at 4º C, do not freeze.

Antibody/Peptide Pairs

cyclin E peptide (MBS443015) is used for blocking the activity of cyclin E antibody (MBS440015)

3D Structure

ModBase 3D Structure for P39949

Host Note

Cyclin E (C19) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminus of rat cyclin E.

Competition Studies

A blocking peptide is also available, MBS443015, for use in competition studies. Each vial contains 100 ug of peptide in 0.5 ml PBS with 0.1% sodium azide and 100 ug BSA.

Applications Tested/Suitable for anti-Ccne1 antibody

Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Application Notes for anti-Ccne1 antibody

WB: 1:200

Product References and Citations for anti-Ccne1 antibody

1.) Song SY, Lee I, Park C, Lee H, Hahm JC, Kang WK. 2005. Neolignans from Saururus chinensis inhibit PC-3 prostate cancer cell growth via apoptosis and senescence-like mechanisms. Int J Mol Med. Oct;16(4):517-23.

1. Bandara LR, Adamczewski JP, Zamanian M, Poon RY, Hunt T, Thangue NB. 1992. Cyclin A recruits p33cdk2 to the cellular transcription factor DRTF1. J Cell Sci Suppl 16: 77-85.2. Desdouets C, Sobczak-Thepot J, Murphy M, Brechot C. 1995. Cyclin A: Function and expression during cell proliferation. Prog Cell Cycle Res 1:115-123.3. Musgrove EA, Sarcevic B, Sutherland RL. 1996. Inducible expression of cyclin D1 in T-47D human breast cancer cells is sufficient for Cdk2 activation and pRB hyperphosphorylation. J Cell Biochem 60(3): 363-378.4. Geng Y, Whorishey W, Park MY, Bronson RT, Medema RH, Li T, Weinberg RA, Sicinski P. 1999. Rescue of cyclin D1 deficiency by knockin cyclin E. Cell 97(6): 767-777.5. Prall OW, Sarcevic B, Musgrove EA, Watts CK, Sutherland RL. 1997. Estrogen-induced activation of Cdk4 and Cdk2 during G1-S phase progression is accompanied by increased cyclin D1 expression and decreased cyclin-dependent kinase inhibitor association with cyclin E-Cdk2. J Biol Chem 272(16): 10882-10894.6. Sauer K, Lehner CF. 1995. The role of cyclin E in the regulation of entry into S phase. Prog Cell Cycle Res 1: 125-139.

Other Notes

Small volumes of anti-Ccne1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Ccne1 purchase

MBS440015 is an antibody targeting Ccne1. Also known as cyclin E (C19) Antibody. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Ccne1. It may not necessarily be applicable to this product.

NCBI GI #

158187539

NCBI GeneID

25729

NCBI Accession #

NP_001094291.1

UniProt Primary Accession #

P39949

UniProt Secondary Accession #

O09138

UniProt Related Accession #

P39949

GenBank Nucleotide #

NM_001100821.1

NCBI Official Full Name

G1/S-specific cyclin-E1

NCBI Official Synonym Full Names

cyclin E1

NCBI Official Symbol

Ccne1

NCBI Official Synonym Symbols

Ccne; CYCLE

UniProt Protein Name

G1/S-specific cyclin-E1

Protein Family

G1/S-specific cyclin

UniProt Gene Name

Ccne1

UniProt Synonym Gene Names

Ccne

UniProt Entry Name

CCNE1_RAT

NCBI Protein Information

G1/S-specific cyclin-E1; cyclin E

NCBI Summary for Ccne1

a cyclin-like protein; may be involved in growth stimulation [RGD, Feb 2006]

UniProt Comments for Ccne1

CCNE1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Two alternatively spliced isoforms have been described.

Protein type: Activator; Cell cycle regulation; Nuclear receptor co-regulator

Cellular Component: centrosome; cyclin-dependent protein kinase holoenzyme complex; cytoplasm; nucleoplasm; nucleus

Molecular Function: cyclin-dependent protein kinase regulator activity; kinase activity; protein complex binding; protein kinase binding

Biological Process: antral ovarian follicle growth; cellular response to nutrient; DNA replication initiation; liver development; negative regulation of transcription from RNA polymerase II promoter; organ regeneration; positive regulation of cell differentiation; protein amino acid phosphorylation; regulation of cell cycle; response to corticosterone stimulus; response to cytokine stimulus; response to drug; response to estradiol stimulus; response to ethanol; response to methylmercury; response to organic cyclic substance; response to organic nitrogen; response to progesterone stimulus; response to purine; response to steroid hormone stimulus; response to vitamin E; synapsis; telomere maintenance; Wnt receptor signaling pathway

Research Articles on Ccne1

1. Our data demonstrated that increased expression of ERK1/2 and cyclinE1 might be involved in the pathogenesis of abnormal rPASMCs proliferation and rat pulmonary vascular remodelling induced by cigarette smoke exposure.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-Ccne1 antibody

Reference Product	PubMed Publications
Cdk2 antibody	>82 publications with Ccne1 and Cdk2
Cdk4 antibody	>37 publications with Ccne1 and Cdk4
Cdkn1a antibody	>35 publications with Ccne1 and Cdkn1a
Cdkn1b antibody	>33 publications with Ccne1 and Cdkn1b
E2f1 antibody	>25 publications with Ccne1 and E2f1
Fbxw7 antibody	>15 publications with Ccne1 and Fbxw7
Rb1 antibody	>13 publications with Ccne1 and Rb1
Cdk1 antibody	>11 publications with Ccne1 and Cdk1
Cdc6 antibody	>9 publications with Ccne1 and Cdc6
Cdc25a antibody	>7 publications with Ccne1 and Cdc25a

Pathways associated with anti-Ccne1 antibody

Products by Pathway	Pathway Diagram
ATM Pathway antibodies	ATM Pathway Diagram
Androgen Receptor Signaling Pathway antibodies	Androgen Receptor Signaling Pathway Diagram
B Cell Receptor Signaling Pathway antibodies	B Cell Receptor Signaling Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Checkpoints Pathway antibodies	Cell Cycle Checkpoints Pathway Diagram
Cell Cycle, Mitotic Pathway antibodies	Cell Cycle, Mitotic Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Cell Cycle - G1/S Transition Pathway antibodies	Cell Cycle - G1/S Transition Pathway Diagram

Organs/Tissues associated with anti-Ccne1 antibody

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>28 publications with Ccne1 and Liver
Lung Antibodies	>27 publications with Ccne1 and Lung
Kidney Antibodies	>12 publications with Ccne1 and Kidney
Brain Antibodies	>12 publications with Ccne1 and Brain
Embryonic Tissue Antibodies	>5 publications with Ccne1 and Embryonic Tissue
Heart Antibodies	>4 publications with Ccne1 and Heart
Placenta Antibodies	>3 publications with Ccne1 and Placenta
Pancreas Antibodies	>2 publications with Ccne1 and Pancreas
Connective Tissue Antibodies	>1 publications with Ccne1 and Connective Tissue