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FBXO43 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS5311991

Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F… More details

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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
FBXO43, Polyclonal Antibody
Full Product Name
FBXO43 Antibody
Product Gene Name
Product Synonym Names
Affinity Purified Rabbit Polyclonal FBXO43 Antibody; Polyclonal FBXO43 antibody; Anti-FBXO43 antibody; FBXO-43 antibody; ERP1 antibody; FBXO-43; FBX43 antibody; F-box only protein 43 antibody; endogenous meiotic inhibitor 2 antibody; FBXO 43 antibody; FBXO43; EMI2 antibody; F-box protein 43 antibody; FBXO 43; FBXO43 antibody; early mitotic inhibitor 2 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Purity/Purification
Antigen-Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
78 kDa
OMIM
609110
3D Structure
ModBase 3D Structure for Q4G163
Immunogen
FBXO43 antibody was raised in rabbit using a tic peptide corresponding to a region within amino acids 646 and 708 of FBXO43
Cross-Reactivity
Human
Applications Tested/Suitable for anti-FBXO43 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-FBXO43 antibody
IF: 1:100-1:200
ICC: 1:100-1:200
IHC-P: 1:100-1:500
WB: 1:500-1:3000

Western Blot (WB) of anti-FBXO43 antibody

Western blot analysis of 30 ug of whole cell lysate (A: NIH-3T3; B: JC) using a 7.5% SDS PAGE gel and FBXO43 antibody at a dilution of 1:1000
Rabbit Anti-FBXO43 Polyclonal Antibody – Cat# MBS5311991

Western Blot (WB) of anti-FBXO43 antibody

Western blot analysis of 30 ug of whole cell lysate (A: 293T) using a 7.5% SDS PAGE gel and FBXO43 antibody at a dilution of 1:1000
Rabbit Anti-FBXO43 Polyclonal Antibody, Western Blot (WB) – Cat# MBS5311991

Immunofluorescence (IF) of anti-FBXO43 antibody

Immunofluorescence analysis of paraformaldehyde-fixed A431, using FBXO43 antibody at 1:200 dilution.
Rabbit Anti-FBXO43 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS5311991

Immunohistochemistry (IHC) of anti-FBXO43 antibody

Immunohistochemical staining of paraffin-embedded Saos-2 xenograft using FBXO43 antibody at a dilution of 1:100
Rabbit Anti-FBXO43 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS5311991

Other Notes

Small volumes of anti-FBXO43 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FBXO43 purchase

MBS5311991 is an antibody targeting FBXO43. Also known as FBXO43 Antibody. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FBXO43. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
F-box only protein 43
NCBI Official Synonym Full Names
F-box protein 43
NCBI Official Symbol
FBXO43
NCBI Official Synonym Symbols
EMI2; ERP1; FBX43
UniProt Protein Name
F-box only protein 43
UniProt Synonym Protein Names
Endogenous meiotic inhibitor 2
Protein Family
UniProt Gene Name
FBXO43
UniProt Synonym Gene Names
EMI2
UniProt Entry Name
FBX43_HUMAN
NCBI Protein Information
F-box only protein 43

NCBI Summary for FBXO43

Members of the F-box protein family, such as FBXO43, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]
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UniProt Comments for FBXO43

FBX43: Required to establish and maintain the arrest of oocytes at the second meiotic metaphase until fertilization. Probably acts by inhibiting the anaphase-promoting complex/cyclosome (APC/C) ubiquitin ligase. Probably recognizes and binds to some phosphorylated proteins and promotes their ubiquitination and degradation (Probable). Part of a SCF (SKP1-cullin-F-box) protein ligase complex. May interact with CDC20.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: nucleus

Molecular Function: zinc ion binding

Biological Process: meiotic cell cycle; negative regulation of meiosis; protein ubiquitination; negative regulation of protein catabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FBXO43 antibody

Organs/Tissues associated with anti-FBXO43 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with FBXO43 and Blood
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