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anti-CXCL8 antibody :: Rabbit IL-8 Polyclonal Antibody

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Catalog # MBS221719
Unit / Price
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  0.1 mg  /  $550 +1 FREE 8GB USB
Product Name

IL-8 (CXCL8), Polyclonal Antibody

Popular Item
Full Product Name

RABBIT ANTI HUMAN INTERLEUKIN-8

Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
OMIM
146930
3D Structure
ModBase 3D Structure for P10145
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Purified
Purified IgG - lyophilised
Concentration
IgG concentration 1.0 mg/ml "after reconstitution" (lot specific)
Reconstitution
Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide.
Immunogen
Recombinant human IL-8
Buffer Solution
Phosphate buffered saline
Antiserum Preparation
Antisera to human IL-8 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species
Human
Preparation and Storage
Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Other Notes
Small volumes of anti-CXCL8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Related Product Information for
anti-CXCL8 antibody
Rabbit anti Human Interleukin-8 antibody recognizes human IL-8 (Interleukin-8), a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-Terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1(NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.
Applications Tested/Suitable for anti-CXCL8 antibody
ELISA (EIA), Functional Assays (FN), Immunofluorescence (IF), Immunohistology Paraffin*, Western Blot (WB)
Application Notes for anti-CXCL8 antibody
ELISA: This purified human IL-8 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human IL-8 antibody as the detection reagent and recombinant human IL-8 as the standard.
Western Blot: This purified human IL-8 antibody may used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human IL-8 as the positive control.
ELISA: Minimum Dilution: 0.5; Maximum Dilution: 2.0ug/ml
Functional Assays: Minimum Dilution: 2.0; Maximum Dilution: 3.0ug/ml
Immunohistology - Paraffin: Minimum Dilution: 0.25; Maximum Dilution: 1.0ug/ml; Application Note: This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose.
Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml

Testing Data #1 of anti-CXCL8 antibody
Staining of formalin fixed, paraffin embedded normal human skin with Rabbit anti Human interleukin-8 (MBS221719) following enzyme mediated antigen retrieval using proteinase K
anti-CXCL8 antibody Testing Data #1 image
Testing Data #2 of anti-CXCL8 antibody
Immunofluorescent staining of BJ human foreskin fibroblastoid cells with Rabbit anti Human interleikin-8 (MBS221719), green; counterstained with tubulin, red and nuclei, blue
anti-CXCL8 antibody Testing Data #2 image
NCBI/Uniprot data below describe general gene information for CXCL8. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
11,338 Da[Similar Products]
NCBI Official Full Name
interleukin-8
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 8
NCBI Official Symbol
CXCL8  [Similar Products]
NCBI Official Synonym Symbols
IL8; NAF; GCP1; LECT; LUCT; NAP1; GCP-1; LYNAP; MDNCF; MONAP; NAP-1
  [Similar Products]
NCBI Protein Information
interleukin-8; T-cell chemotactic factor; alveolar macrophage chemotactic factor I; beta endothelial cell-derived neutrophil activating peptide; beta-thromboglobulin-like protein; emoctakin; granulocyte chemotactic protein 1; interleukin 8; lung giant cell carcinoma-derived chemotactic protein; lymphocyte derived neutrophil activating peptide; monocyte-derived neutrophil chemotactic factor; monocyte-derived neutrophil-activating peptide; neutrophil-activating peptide 1; small inducible cytokine subfamily B, member 8; tumor necrosis factor-induced gene 1
UniProt Protein Name
Interleukin-8
UniProt Synonym Protein Names
C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1; GCP-1; Monocyte-derived neutrophil chemotactic factor; MDNCF; Monocyte-derived neutrophil-activating peptide; MONAP; Neutrophil-activating protein 1; NAP-1; Protein 3-10C; T-cell chemotactic factorCleaved into the following 7 chains:MDNCF-aAlternative name(s):GCP/IL-8 protein IV; IL8/NAP1 form I
Protein Family
UniProt Gene Name
CXCL8  [Similar Products]
UniProt Synonym Gene Names
IL8; IL-8; GCP-1; MDNCF; MONAP; NAP-1; LYNAP; NAF  [Similar Products]
UniProt Entry Name
IL8_HUMAN
NCBI Summary for CXCL8
The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]
UniProt Comments for CXCL8
IL8: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. Belongs to the intercrine alpha (chemokine CxC) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13-q21

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity; interleukin-8 receptor binding

Biological Process: regulation of cell adhesion; neutrophil chemotaxis; neutrophil activation; negative regulation of G-protein coupled receptor protein signaling pathway; unfolded protein response; calcium-mediated signaling; chemotaxis; signal transduction; regulation of retroviral genome replication; induction of positive chemotaxis; negative regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; response to molecule of bacterial origin; receptor internalization; immune response; angiogenesis; inflammatory response; cell motility; cell cycle arrest; embryonic gut development
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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