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Neomycin Phosphotransferase II Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS612660

Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics… More details

$1155 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Neomycin Phosphotransferase II, Polyclonal Antibody
Full Product Name
Neomycin Phosphotransferase II (NPT II)
Product Synonym Names
Anti -Neomycin Phosphotransferase II (NPT II)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Specificity
Recognizes bacterial NPT II, Mr ~30kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length Neomycin Phosphotransferase II (NPT II)
Applications Tested/Suitable for anti-Neomycin Phosphotransferase II antibody
ELISA (EL/EIA), Western Blot (WB)
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1-2ug/ml. Detects NPT II in RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene. RIPA lysates of transiently transfected Cos-1 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with N2008-05B. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.

Western Blot (WB) of anti-Neomycin Phosphotransferase II antibody

Western Blot from a previous lot. RIPA lysates of transiently transfected Cos-1 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS612660 (1ug/ml). Proteins were visualized using IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Lane 1: pUSE untransfected control; Lane 2: Transfected cells. Arrow indicates NPT II (30kD).
Rabbit Anti-Neomycin Phosphotransferase II Polyclonal Antibody – Cat# MBS612660

Western Blot (WB) of anti-Neomycin Phosphotransferase II antibody

Western Blot from this lot. A431 lysate was resolved by electrophoresis, transferred to membrane and probed with MBS612660 (1:500). Proteins were visualized using a donkey anti-rabbit IgG (HRP) and a chemiluminescence detection system. Lane 1: pUSE untransfected control; Lane 2: Transfected cells.
Rabbit Anti-Neomycin Phosphotransferase II Polyclonal Antibody, Western Blot (WB) – Cat# MBS612660

Other Notes

Small volumes of anti-Neomycin Phosphotransferase II antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Neomycin Phosphotransferase II purchase

MBS612660 is an antibody targeting Neomycin Phosphotransferase II. Also known as Neomycin Phosphotransferase II (NPT II). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for Neomycin Phosphotransferase II. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Official Full Name
neomycin phosphotransferase II

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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