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Protease Inhibitor 15 (PI15) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS835109

MBS835109 is an antibody targeting PI15. Also known as Protease Inhibitor 15 antibody. Host: Rabbit. Affinity purified Rabbit polyclonal Protease Inhibitor 15 antibody More details

$1565 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Protease Inhibitor 15 (PI15), Polyclonal Antibody
Full Product Name
Protease Inhibitor 15 antibody
Product Gene Name
Product Synonym Names
Polyclonal Protease Inhibitor 15; Anti-Protease Inhibitor 15; Protease Inhibitor 15; P24TI; 25 kDa trypsin inhibitor; protease inhibitor 15; P25TI; DKFZp686F0366; sugarCrisp; PI15; CRISP8; CRISP-8; Protease Inhibitor -15
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Purity/Purification
Protease Inhibitor 15 antibody was purified by antigen-affinity chromatography
Concentration
0.6 mg/ml (lot specific)
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Molecular Weight
29 kDa
OMIM
607076
3D Structure
ModBase 3D Structure for O43692
Cross-Reactivity
Human
Immunogen
Protease Inhibitor 15 antibody was raised in rabbit using a synthetic peptide contain a sequence corresponding to a region within amino acids 195 and 208  of Protease Inhibitor 15
Applications Tested/Suitable for anti-PI15 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes for anti-PI15 antibody
IF:1:100-1:500
ICC:1:100-1:500
IHC-P: 1:100-1:500
WB: 1:500-1:3000

Immunofluorescence (IF) of anti-PI15 antibody

Immunofluorescence analysis of methanol-fixed HeLa, using Protease Inhibitor 15 antibody at 1:500 dilution.
Rabbit Anti-PI15 Polyclonal Antibody – Cat# MBS835109

Western Blot (WB) of anti-PI15 antibody

Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 12 % SDS PAGE gel and Protease Inhibitor 15 antibody at a dilution of 1:1000
Rabbit Anti-PI15 Polyclonal Antibody, Western Blot (WB) – Cat# MBS835109

Immunohistochemistry (IHC) of anti-PI15 antibody

Immunohistochemical staining of paraffin-embedded Breast ca using Protease Inhibitor 15 antibody at a dilution of 1:250
Rabbit Anti-PI15 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS835109

Other Notes

Small volumes of anti-PI15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for PI15. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
peptidase inhibitor 15 preproprotein
NCBI Official Synonym Full Names
peptidase inhibitor 15
NCBI Official Symbol
PI15
NCBI Official Synonym Symbols
P24TI; P25TI; CRISP8
UniProt Protein Name
Peptidase inhibitor 15
UniProt Synonym Protein Names
25 kDa trypsin inhibitor; p25TI; Cysteine-rich secretory protein 8; CRISP-8; SugarCrisp
Protein Family
UniProt Gene Name
PI15
UniProt Synonym Gene Names
CRISP8; P25TI; PI-15; p25TI; CRISP-8
UniProt Entry Name
PI15_HUMAN
NCBI Protein Information
peptidase inhibitor 15

NCBI Summary for PI15

This gene encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system. [provided by RefSeq, Jul 2008]
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UniProt Comments for PI15

PI15: Serine protease inhibitor which displays weak inhibitory activity against trypsin. Belongs to the CRISP family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8q21.11

Molecular Function: protease inhibitor activity

Biological Process: negative regulation of peptidase activity
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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