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Protein Kinase A, Substrate (ypkA) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS627817

A class of kinases that regulates an array of physiological processes and includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase, protein kinase C, Akt and RSK shares a substrate specificity characterized by arginine at position -3… More details

$835 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Protein Kinase A, Substrate (ypkA), Polyclonal Antibody
Full Product Name
Protein Kinase A, Substrate, Phosphorylated (Ser/Thr) (PKA)
Product Gene Name
Product Synonym Names
Anti -Protein Kinase A, Substrate, Phosphorylated (Ser/Thr) (PKA)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity/Purification
Affinity Purified
Purified by protein A and peptide affinity chromatography.
Specificity
Detects peptides and proteins containing a phospho-Ser/Thr residue with arginine at the -3 position. It is a useful tool in identifying new substrates of AGC family kinases, including PKA and PKC. It does not recognize the non-phosphorylated PKA substrate motif.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Chromosome Location
NC_004836.1 (63100..65298)
Immunogen
Synthetic phospho- PKA substrate peptide.
Applications Tested/Suitable for anti-ypkA antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes for anti-ypkA antibody
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin): 1:100
ELISA: 1:500

Western Blot (WB) of anti-ypkA antibody

Western Blot analysis of A431 cells, untreated or calyculin A-treated, using MBS627817.
Rabbit Anti-ypkA Polyclonal Antibody – Cat# MBS627817

Western Blot (WB) of anti-ypkA antibody

Western Blot analysis of extracts from A431 cells, phosphorylated in vitro by protein kinase A, Erk2 or cdc2/cyclin A, plus or minus PKA inhibitor (PKI), using MBS627817.
Rabbit Anti-ypkA Polyclonal Antibody, Western Blot (WB) – Cat# MBS627817

Immunohistochemistry (IHC) of anti-ypkA antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using MBS627817.
Rabbit Anti-ypkA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS627817

Immunohistochemistry (IHC) of anti-ypkA antibody

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MBS627817.
Rabbit Anti-ypkA Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS627817

Other Notes

Small volumes of anti-ypkA antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ypkA purchase

MBS627817 is an antibody targeting ypkA. Also known as Protein Kinase A, Substrate, Phosphorylated (Ser/Thr) (PKA). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ypkA. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein kinase A
NCBI Official Symbol
ypkA
Protein Family
NCBI Protein Information
similar to Yersinia pseudotuberculosis protein kinase A

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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