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ARHGEF3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8242512

MBS8242512 is an antibody targeting ARHGEF3. Also known as Anti-XPLN Antibody. Reactive in Human, Monkey. Host: Rabbit. Rabbit polyclonal antibody to XPLN More details

$225 / 0.03 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
XPLN (ARHGEF3), Polyclonal Antibody
Full Product Name
Anti-XPLN Antibody
Product Gene Name
anti-ARHGEF3 antibody
Product Synonym Names
Rho guanine nucleotide exchange factor 3; Exchange factor found in platelets and leukemic and neuronal tissues; XPLN
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human, Monkey
Form/Format
Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% Sodium Azide.
Purity/Purification
The antibody was purified by affinity chromatography.
Specificity
Recognizes endogenous levels of XPLN protein.
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Antibody/Peptide Pairs
XPLN peptide (MBS8244515) is used for blocking the activity of XPLN antibody (MBS8242512)
Molecular Weight
36,981 Da
OMIM
612115
3D Structure
ModBase 3D Structure for Q9NR81
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human XPLN. The exact sequence is proprietary.
Applications Tested/Suitable for anti-ARHGEF3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes for anti-ARHGEF3 antibody
Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500)

Western Blot (WB) of anti-ARHGEF3 antibody

Western blot analysis of XPLN expression in MCF7 (A), COLO205 (B) whole cell lysates.
Rabbit Anti-ARHGEF3 Polyclonal Antibody – Cat# MBS8242512

Immunohistochemistry (IHC) of anti-ARHGEF3 antibody

Immunohistochemical analysis of XPLN staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Rabbit Anti-ARHGEF3 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS8242512

Immunofluorescence (IF) of anti-ARHGEF3 antibody

Immunofluorescent analysis of XPLN staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Rabbit Anti-ARHGEF3 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS8242512

Other Notes

Small volumes of anti-ARHGEF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ARHGEF3. It may not necessarily be applicable to this product.

NCBI GI #
52782760
NCBI GeneID
50650
NCBI Accession #
Q9NR81.1
UniProt Primary Accession #
Q9NR81
UniProt Secondary Accession #
Q4FZB6; Q4QQI5; Q4QQQ0; Q59F00; Q6NUN3; Q7Z4U2; Q7Z5T2; Q9H7T4; A8K5U7
UniProt Related Accession #
Q9NR81
NCBI Official Full Name
Rho guanine nucleotide exchange factor 3
NCBI Official Synonym Full Names
Rho guanine nucleotide exchange factor 3
NCBI Official Symbol
ARHGEF3
NCBI Official Synonym Symbols
GEF3; STA3; XPLN
UniProt Protein Name
Rho guanine nucleotide exchange factor 3
UniProt Synonym Protein Names
Exchange factor found in platelets and leukemic and neuronal tissues; XPLN
Protein Family
Rho guanine nucleotide exchange factor
UniProt Gene Name
ARHGEF3
UniProt Synonym Gene Names
XPLN
UniProt Entry Name
ARHG3_HUMAN
NCBI Protein Information
rho guanine nucleotide exchange factor 3

NCBI Summary for ARHGEF3

Rho-like GTPases are involved in a variety of cellular processes, and they are activated by binding GTP and inactivated by conversion of GTP to GDP by their intrinsic GTPase activity. Guanine nucleotide exchange factors (GEFs) accelerate the GTPase activity of Rho GTPases by catalyzing their release of bound GDP. This gene encodes a guanine nucleotide exchange factor, which specifically activates two members of the Rho GTPase family: RHOA and RHOB, both of which have a role in bone cell biology. It has been identified that genetic variation in this gene plays a role in the determination of bone mineral density (BMD), indicating the implication of this gene in postmenopausal osteoporosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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UniProt Comments for ARHGEF3

ARHGEF3: Acts as guanine nucleotide exchange factor (GEF) for RhoA and RhoB GTPases. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, Rac/Rho; Motility/polarity/chemotaxis; GAPs

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: cytosol; intracellular

Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity

Biological Process: nerve growth factor receptor signaling pathway; positive regulation of apoptosis; positive regulation of GTPase activity; regulation of Rho protein signal transduction; regulation of small GTPase mediated signal transduction; Rho protein signal transduction; small GTPase mediated signal transduction
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Research Articles on ARHGEF3

1. ARHGEF3 and RHOA are potential regulators of a number of genes in bone cells, with influences on ACTA2 evident in both osteoblast-like and osteoclast-like cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ARHGEF3 antibody

 Products by Pathway  Pathway Diagram
 Cell Death Signalling Via NRAGE, NRIF And NADE Pathway antibodies  Cell Death Signalling Via NRAGE, NRIF And NADE Pathway Diagram
 G Alpha (12/13) Signalling Events Pathway antibodies  G Alpha (12/13) Signalling Events Pathway Diagram
 GPCR Downstream Signaling Pathway antibodies  GPCR Downstream Signaling Pathway Diagram
 NRAGE Signals Death Through JNK Pathway antibodies  NRAGE Signals Death Through JNK Pathway Diagram
 Regulation Of RhoA Activity Pathway antibodies  Regulation Of RhoA Activity Pathway Diagram
 Rho GTPase Cycle Pathway antibodies  Rho GTPase Cycle Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By GPCR Pathway antibodies  Signaling By GPCR Pathway Diagram
 Signaling By Rho GTPases Pathway antibodies  Signaling By Rho GTPases Pathway Diagram
 Signalling By NGF Pathway antibodies  Signalling By NGF Pathway Diagram

Diseases associated with anti-ARHGEF3 antibody

 Disease Name  Pubmed Publications
 Anemia Antibodies  >2 publications with ARHGEF3 and Anemia
 Disease Models, Animal Antibodies  >1 publications with ARHGEF3 and Disease Models, Animal
 Necrosis Antibodies  >1 publications with ARHGEF3 and Necrosis

Organs/Tissues associated with anti-ARHGEF3 antibody

 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >5 publications with ARHGEF3 and Bone
 Blood Antibodies  >3 publications with ARHGEF3 and Blood
 Muscle Antibodies  >2 publications with ARHGEF3 and Muscle
 Kidney Antibodies  >2 publications with ARHGEF3 and Kidney
 Brain Antibodies  >2 publications with ARHGEF3 and Brain
 Prostate Antibodies  >1 publications with ARHGEF3 and Prostate
 Cervix Antibodies  >1 publications with ARHGEF3 and Cervix
 Parathyroid Antibodies  >1 publications with ARHGEF3 and Parathyroid
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