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P15 INK (CDKN2B) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9600167

Description: p15-INK4B a potent inhibitor that nteracts strongly with CDK4 and CDK6.Function: Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.Subunit Structure: Heterodimer of CDKN2B with CDK4… More details

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Product Overview

Online Datasheet in PDF
Product Name
p15 INK (CDKN2B), Polyclonal Antibody
Full Product Name
p15 INK Antibody
Product Gene Name
anti-CDKN2B antibody
Product Synonym Names
CDK inhibitory protein; CDK4B Inhibitor; Cdkn2b; CDN2B_HUMAN; Cyclin dependent kinase 4 inhibitor B; Cyclin Dependent Kinase Inhibitor 2B; Cyclin dependent kinase inhibitor 2B p15 inhibits CDK4; Cyclin dependent kinases 4 and 6 binding protein; Cyclin-dependent kinase 4 inhibitor B; INK4B; MTS 2; MTS-2; MTS2; Multiple tumor suppressor 2; Multiple Tumor Supressor 2; OTTHUMP00000021154; OTTHUMP00000021155; p14 CDK inhibitor; p14 INK4b; p14-INK4b; P15; p15 CDK inhibitor; p15 inhibits CDK4; p15 INK4b; p15-INK4b; p15INK4B; TP 15; TP15
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
p15 INK antibody detects endogenous levels of total p15 INK
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
p15 INK4b peptide (MBS9614719) is used for blocking the activity of p15 INK4b antibody (MBS9600167)
Molecular Weight
Observed: 14 kDa
Predicted: 15 kDa
3D Structure
ModBase 3D Structure for P42772
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human p15 INK
Tissue Specificity
Isoform 2 is expressed in normal (keratinocytes, fibroblasts) and tumor cell lines.
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-CDKN2B antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-CDKN2B antibody
WB: 1:500-1:3000
IHC: 1:50-1:200
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-CDKN2B antibody

MBS9600167 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-CDKN2B Polyclonal Antibody – Cat# MBS9600167

Western Blot (WB) of anti-CDKN2B antibody

Western blot analysis on HeLa cell lysate using p15 INK Antibody. The lane on the left is treated with the antigen-specific peptide.
Rabbit Anti-CDKN2B Polyclonal Antibody, Western Blot (WB) – Cat# MBS9600167

Immunofluorescence (IF) of anti-CDKN2B antibody

MBS9600167 staining HEK 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-CDKN2B Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS9600167

Product References and Citations for anti-CDKN2B antibody

Chen D, Zhang Z, Mao C, Zhou Y, Yu L, Yin Y, Wu S, Mou X, Zhu Y; Journal: Cell Immunol. ANRIL inhibits p15(INK4b) through the TGFbeta1 signaling pathway in human esophageal squamous cell carcinoma.

Other Notes

Small volumes of anti-CDKN2B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CDKN2B purchase

MBS9600167 is an antibody targeting CDKN2B. Also known as p15 INK Antibody. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for CDKN2B. It may not necessarily be applicable to this product.

NCBI GI #
17981694
NCBI GeneID
1030
NCBI Accession #
NP_004927.2
UniProt Primary Accession #
P42772
UniProt Secondary Accession #
O15125; Q6FI09
UniProt Related Accession #
P42772
GenBank Nucleotide #
NM_004936.3
NCBI Official Full Name
cyclin-dependent kinase 4 inhibitor B isoform 1
NCBI Official Synonym Full Names
cyclin dependent kinase inhibitor 2B
NCBI Official Symbol
CDKN2B
NCBI Official Synonym Symbols
P15; MTS2; TP15; CDK4I; INK4B; p15INK4b
UniProt Protein Name
Cyclin-dependent kinase 4 inhibitor B
UniProt Synonym Protein Names
Multiple tumor suppressor 2; MTS-2; p14-INK4b; p15-INK4b; p15INK4B
Protein Family
Cyclin-dependent kinase 4 inhibitor
UniProt Gene Name
CDKN2B
UniProt Synonym Gene Names
MTS2; MTS-2; p15INK4B
NCBI Protein Information
cyclin-dependent kinase 4 inhibitor B

NCBI Summary for CDKN2B

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]
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UniProt Comments for CDKN2B

Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.

Research Articles on CDKN2B

1. CDKN2B expression in HPDE6-C7 human pancreatic ductal cells is induced by oncogenic KRAS via TGF-beta signaling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CDKN2B antibody

 Reference Product  PubMed Publications
 CDK4 antibody  >117 publications with CDKN2B and CDK4
 ZBTB17 antibody  >7 publications with CDKN2B and ZBTB17

Pathways associated with anti-CDKN2B antibody

 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cyclin D Associated Events In G1 Pathway antibodies  Cyclin D Associated Events In G1 Pathway Diagram
 G1 Phase Pathway antibodies  G1 Phase Pathway Diagram
 G1 To S Cell Cycle Control Pathway antibodies  G1 To S Cell Cycle Control Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram

Diseases associated with anti-CDKN2B antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >622 publications with CDKN2B and Neoplasms
 Leukemia Antibodies  >205 publications with CDKN2B and Leukemia
 Immune System Diseases Antibodies  >160 publications with CDKN2B and Immune System Diseases
 Carcinoma Antibodies  >151 publications with CDKN2B and Carcinoma
 Lymphoma Antibodies  >149 publications with CDKN2B and Lymphoma
 Chromosome Aberrations Antibodies  >101 publications with CDKN2B and Chromosome Aberrations
 Leukemia, Myeloid, Acute Antibodies  >88 publications with CDKN2B and Leukemia, Myeloid, Acute
 Glioma Antibodies  >58 publications with CDKN2B and Glioma
 Carcinoma, Squamous Cell Antibodies  >48 publications with CDKN2B and Carcinoma, Squamous Cell
 Myelodysplastic Syndromes Antibodies  >44 publications with CDKN2B and Myelodysplastic Syndromes

Organs/Tissues associated with anti-CDKN2B antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >62 publications with CDKN2B and Brain
 Lung Antibodies  >50 publications with CDKN2B and Lung
 Liver Antibodies  >44 publications with CDKN2B and Liver
 Skin Antibodies  >34 publications with CDKN2B and Skin
 Muscle Antibodies  >22 publications with CDKN2B and Muscle
 Prostate Antibodies  >18 publications with CDKN2B and Prostate
 Bladder Antibodies  >18 publications with CDKN2B and Bladder
 Stomach Antibodies  >15 publications with CDKN2B and Stomach
 Embryonic Tissue Antibodies  >10 publications with CDKN2B and Embryonic Tissue
 Kidney Antibodies  >9 publications with CDKN2B and Kidney
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