Goat Anti-CSTF3 Polyclonal Antibody – Cat# MBS423071

(0.1ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

CSTF3 Antibody – Goat Polyclonal

Name: CSTF3 Antibody; Polyclonal; Goat; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS423071
Price: 450 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423071

MBS423071 is an antibody targeting CSTF3. Also known as Goat anti-CSTF3 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CSTF3, Polyclonal Antibody

Full Product Name

Goat anti-CSTF3 Antibody

Product Gene Name

anti-CSTF3 antibody

Product Synonym Names

CSTF3; cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa; CSTF-77; MGC117398; MGC43001; MGC75122; CSTF 77 kDa subunit; cleavage stimulation factor subunit 3; cleavage stimulation factor subunit 3, isoform 1; cleavage stimulation factor subunit 3 antibody; cleavage stimulation factor subunit 3; isoform 1 antibody; cleavage stimulation factor; 3' pre-RNA; subunit 3; 77kDa antibody; CSTF 77 kDa subunit antibody; CSTF-77 antibody; MGC117398 antibody; MGC43001 antibody; MGC75122 antibody; CSTF3 antibody

Immunogen Sequence

RETKGKLPSY KE

Clonality

Polyclonal

Host

Goat

Species Reactivity

Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog

Form/Format

Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Purity/Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

100ug specific antibody in 200ul (lot specific)

Preparation and Storage

Aliquot and store at -20 degree C. Minimize freezing and thawing.

Antibody/Peptide Pairs

CSTF3 peptide (MBS427931) is used for blocking the activity of CSTF3 antibody (MBS423071)

Molecular Weight

4,952 Da

OMIM

600367

Immunogen

Peptide with sequence C-RETKGKLPSYKE, from the internal region of the protein sequence according to NP_001317.1.

Epitope

Internal region

Note

This antibody is expected to recognize isoform 1 (NP_001317.1) only.

ISO Certification

Manufactured in an ISO 9001:2008 Certified Laboratory.

Applications Tested/Suitable for anti-CSTF3 antibody

Peptide ELISA (EIA), Western Blot (WB)

Application Notes for anti-CSTF3 antibody

Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 75kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 82.9kDa according to NP_001317.1). The observed molecular weight corresponds to the expected size of 77kDa. Recommended concentration: 0. 03-0.1ug/ml.

Other Notes

Small volumes of anti-CSTF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CSTF3. It may not necessarily be applicable to this product.

NCBI GI #

4557495

NCBI GeneID

1479

NCBI Accession #

NP_001317.1

UniProt Secondary Accession #

Q32P22; Q96FQ8; Q96QD6; Q96QK4; A8K471; D3DR04; E9PB40

UniProt Related Accession #

Q12996

GenBank Nucleotide #

NM_001326.2

NCBI Official Full Name

cleavage stimulation factor subunit 3 isoform 1

NCBI Official Synonym Full Names

cleavage stimulation factor subunit 3

NCBI Official Symbol

CSTF3

NCBI Official Synonym Symbols

CSTF-77

UniProt Protein Name

Cleavage stimulation factor subunit 3

UniProt Synonym Protein Names

CF-1 77 kDa subunit; Cleavage stimulation factor 77 kDa subunit; CSTF 77 kDa subunit; CstF-77

Protein Family

Cleavage stimulation factor

UniProt Gene Name

CSTF3

UniProt Synonym Gene Names

CSTF 77 kDa subunit; CstF-77

UniProt Entry Name

CSTF3_HUMAN

NCBI Protein Information

cleavage stimulation factor subunit 3

NCBI Summary for CSTF3

The protein encoded by this gene is one of three (including CSTF1 and CSTF2) cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in the polyadenylation and 3' end cleavage of pre-mRNAs. The encoded protein functions as a homodimer and interacts directly with both CSTF1 and CSTF2 in the CSTF complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

UniProt Comments for CSTF3

CstF-77: One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: nucleoplasm; nucleus

Molecular Function: mRNA binding; protein binding; RNA binding

Biological Process: mRNA 3'-end processing; mRNA cleavage; mRNA polyadenylation; nuclear mRNA splicing, via spliceosome; termination of RNA polymerase II transcription

Research Articles on CSTF3

1. Thus, the conserved intronic pA of the CstF-77 gene may function as a sensor for cellular C/P and splicing activities, controlling the homeostasis of CstF-77 and C/P activity and impacting cell proliferation and differentiation.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CSTF3 antibody

Products by Pathway	Pathway Diagram
Cleavage Of Growing Transcript In The Termination Region Pathway antibodies	Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Processing Of Capped Intron-Containing Pre-mRNA Pathway antibodies	Processing Of Capped Intron-Containing Pre-mRNA Pathway Diagram
Processing Of Capped Intronless Pre-mRNA Pathway antibodies	Processing Of Capped Intronless Pre-mRNA Pathway Diagram
Processing Of Intronless Pre-mRNAs Pathway antibodies	Processing Of Intronless Pre-mRNAs Pathway Diagram
RNA Polymerase II Transcription Pathway antibodies	RNA Polymerase II Transcription Pathway Diagram
RNA Polymerase II Transcription Termination Pathway antibodies	RNA Polymerase II Transcription Termination Pathway Diagram
MRNA 3'-end Processing Pathway antibodies	MRNA 3'-end Processing Pathway Diagram
MRNA Splicing Pathway antibodies	MRNA Splicing Pathway Diagram
MRNA Splicing - Major Pathway antibodies	MRNA Splicing - Major Pathway Diagram

Diseases associated with anti-CSTF3 antibody

Disease Name	Pubmed Publications
Infertility, Male Antibodies	>1 publications with CSTF3 and Infertility, Male
Congenital Abnormalities Antibodies	>1 publications with CSTF3 and Congenital Abnormalities

Organs/Tissues associated with anti-CSTF3 antibody

Organ/Tissue Name	Pubmed Publications
Stomach Antibodies	>1 publications with CSTF3 and Stomach