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THIF Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS7158878

MBS7158878 is an antibody targeting THIF. Also known as Rabbit anti-Escherichia coli (strain K12) THIF Polyclonal Antibody. Reactive in E Coli (strain K12). Host: Rabbit. More details

Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
THIF, Polyclonal Antibody
Full Product Name
Rabbit anti-Escherichia coli (strain K12) THIF Polyclonal Antibody
Product Gene Name
Product Synonym Names
THIF Antibody; Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73; thiF b3992 JW3956
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
E Coli (strain K12)
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Purity/Purification
Antigen-affinity
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
Molecular Weight
26,970 Da
3D Structure
ModBase 3D Structure for P30138
Immunogen
Recombinant Escherichia coli (strain K12) thiF protein
Conjugation
Non-conjugate
Modification
Unmodified
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-THIF antibody
ELISA (EIA), WB (Western Blot) (ensure identification of antigen)

Other Notes

Small volumes of anti-THIF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for THIF. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
GenBank Nucleotide #
NCBI Official Full Name
adenylyltransferase, modifies ThiS C-terminus
NCBI Official Symbol
thiF
NCBI Official Synonym Symbols
ECK3984; JW3956; thiA
UniProt Protein Name
Sulfur carrier protein ThiS adenylyltransferase
UniProt Gene Name
thiF
NCBI Protein Information
adenylyltransferase, modifies ThiS C-terminus

NCBI Summary for THIF

The ThiF sequence has been confirmed using MS/MS, however since use of the start codon was forced during cloning, this is not a verification of the start site: the measured mass of the intact protein is 26970.2 indicating that the initial Met is not removed (Kelleher, 1998). [More information is available at EcoGene: EG11587]. The ThiF protein catalyzes the adenylation of the ThiS protein as part of the formation of the thiazole moiety during thiamin biosynthesis . [More information is available at EcoCyc: EG11587].
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UniProt Comments for THIF

Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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