Rabbit Anti-EPHB2 Polyclonal Antibody – Cat# MBS502033

Ephrinb (Tyr331) (EPHB2) Antibody – Rabbit Polyclonal

Name: EPHB2 Antibody; Polyclonal; Rabbit; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502033
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502033

MBS502033 is an antibody targeting EPHB2. Also known as Anti-Phospho-Tyr331 EphrinB. Reactive in Rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

EphrinB (Tyr331) (EPHB2), Polyclonal Antibody

Full Product Name

Anti-Phospho-Tyr331 EphrinB

Product Gene Name

anti-EPHB2 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Rat

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

Specificity

Specific for the ~46k EphrinB protein phosphorylated at Tyr331. Immunolabeling is blocked by lambda-phosphatase treatment.

Preparation and Storage

Store at -20 degree C; stable for at least one year.

Molecular Weight

Chromosome Location

Chromosome: 21; NC_006108.3 (5941301..6038945, complement). Location: chromosome: 21

3D Structure

ModBase 3D Structure for P28693

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr331 of chicken EphrinB. Note: Chicken Tyr331 is the homolog of mouse and rat Tyr343, human Tyr344 and also Xenopus Tyr324.

Immunogen Information

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr331 conjugated to KLH

Immunogen Species

Chicken

Reactivity Assumed Based on 100% Sequence Homology

Bovine, chicken, human, mouse, Xenopus and zebra fish

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, chicken, human, mouse, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Biological Significance

EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vanderhaeghen, 1998; Dufour et al., 2003; Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work demonstrated that Tyr331 of EphrinB was phosphorylated in HEK293 cells after timulation by the soluble EphB2 receptor tyrosine kinase (Kalo et al., 2001).

Applications Tested/Suitable for anti-EPHB2 antibody

Western Blot (WB)

Application Notes for anti-EPHB2 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000

Product References and Citations for anti-EPHB2 antibody

• Bong, Y.S., Park, Y.H., Lee, H.S., Mood, K., Ishimura, A. and Daar, I.O. Tyr-298 in ephrinB1 is critical for an interaction with the Grb4 adaptor protein, Biochem. J. 377:499-507 (2004).
• Dufour, A., Seibt, J., Passante, L., Depaepe, V., Ciossek, T., Frisen, J., Kullander, K., Flanagan, J.G., Polleux, F. and Vanderhaeghen, P. Area specificity and topography of thalamocortical projections are controlled by ephrin/Eph genes, Neuron 39:453-465 (2003).
• Flanagan, J.G. and Vanderhaeghen, P. The ephrins and Eph receptors in neural development, Annu. Rev. Neurosci. 21:309-345 (1998).
• Oike, Y., Ito, Y., Hamada, K., Zhang, X.Q., Miyata, K., Arai, F., Inada, T., Araki, K., Nakagata, N., Takeya, M., Kisanuki, Y.Y., Yanagisawa, M., Gale, N.W. and Suda, T, Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells, Blood 100:1326-1333 (2002).
• Palmer, A., Zimmer, M., Erdmann, K.S., Eulenburg, V., Porthin, A., Heumann, R., Deutsch, U. and Klein, R Ephrin B phosphorylation and reverse signaling: regulation by Src kinases and PTP-BL Phosphatase, Mol Cell 9:725-737 (2002).

Other Notes

Small volumes of anti-EPHB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EPHB2. It may not necessarily be applicable to this product.

NCBI GI #

46395489

NCBI GeneID

396513

NCBI Accession #

NP_996834.1

UniProt Primary Accession #

P28693

UniProt Related Accession #

P28693

GenBank Nucleotide #

NM_206951.3

NCBI Official Full Name

ephrin type-B receptor 2

NCBI Official Synonym Full Names

EPH receptor B2

NCBI Official Symbol

EPHB2

UniProt Protein Name

Ephrin type-B receptor 2

UniProt Synonym Protein Names

EPH-like kinase 5

Protein Family

Ephrin type-B receptor

UniProt Gene Name

EPHB2

UniProt Synonym Gene Names

CEK5; EK5; cEK5

UniProt Entry Name

EPHB2_CHICK

NCBI Protein Information

ephrin type-B receptor 2; EK5; cEK5; EPH-like kinase 5; embryo kinase 5 protein CEK5

UniProt Comments for EPHB2

Function: Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Beside axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses

By similarity.

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses

By similarity.

Subcellular location: Cell membrane; Single-pass type I membrane protein

By similarity. Cell projection › axon

By similarity. Cell projection › dendrite

By similarity.

Tissue specificity: Wide tissue distribution throughout development and sustained expression in adult brain. The longer form (CEK5+) is specifically expressed in the central nervous system.

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.Contains 1 Eph LBD (Eph ligand-binding) domain.Contains 2 fibronectin type-III domains.Contains 1 protein kinase domain.Contains 1 SAM (sterile alpha motif) domain.

Sequence caution: The sequence AAA48667.1 differs from that shown. Reason: Erroneous initiation.

Research Articles on EPHB2

1. Downregulation of EphB2/B3 during the narrow window of early embryogenesis may interfere with normal somitogenesis, preventing migration of embryonic body wall ventrally and thus causing omphalocele.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EPHB2 antibody

Reference Product	PubMed Publications
SRC antibody	>27 publications with EPHB2 and SRC
EFNB1 antibody	>12 publications with EPHB2 and EFNB1
EFNB2 antibody	>9 publications with EPHB2 and EFNB2
TIAM1 antibody	>2 publications with EPHB2 and TIAM1
RRAS2 antibody	>1 publications with EPHB2 and RRAS2
GRB10 antibody	>1 publications with EPHB2 and GRB10

Pathways associated with anti-EPHB2 antibody

Products by Pathway	Pathway Diagram
NLR Proteins Pathway antibodies	NLR Proteins Pathway Diagram

Diseases associated with anti-EPHB2 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>105 publications with EPHB2 and Neoplasms
Intestinal Diseases Antibodies	>45 publications with EPHB2 and Intestinal Diseases
Nervous System Diseases Antibodies	>29 publications with EPHB2 and Nervous System Diseases
Disease Models, Animal Antibodies	>12 publications with EPHB2 and Disease Models, Animal
Congenital Abnormalities Antibodies	>11 publications with EPHB2 and Congenital Abnormalities
Cardiovascular Diseases Antibodies	>7 publications with EPHB2 and Cardiovascular Diseases
Necrosis Antibodies	>7 publications with EPHB2 and Necrosis
Hyperalgesia Antibodies	>6 publications with EPHB2 and Hyperalgesia
Neoplasms, Experimental Antibodies	>5 publications with EPHB2 and Neoplasms, Experimental
Breast Neoplasms Antibodies	>4 publications with EPHB2 and Breast Neoplasms

Organs/Tissues associated with anti-EPHB2 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>121 publications with EPHB2 and Brain
Embryonic Tissue Antibodies	>44 publications with EPHB2 and Embryonic Tissue
Head Antibodies	>14 publications with EPHB2 and Head
Ovary Antibodies	>1 publications with EPHB2 and Ovary
Limb Antibodies	>1 publications with EPHB2 and Limb