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GPANK1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9204019

MBS9204019 is an antibody targeting GPANK1. Also known as BAT4 Antibody (C-term). Reactive in Human. Host: Rabbit. BAT4 is thought to be involved in some aspects of immunity. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BAT4 (GPANK1), Polyclonal Antibody
Full Product Name
BAT4 Antibody (C-term)
Product Gene Name
Product Synonym Names
G patch domain and ankyrin repeat-containing protein 1; Ankyrin repeat domain-containing protein 59; G patch domain-containing protein 10; HLA-B-associated transcript 4; Protein G5; GPANK1; ANKRD59; BAT4; G5; GPATCH10
Clonality
Polyclonal
Isotype
Rabbit Ig
Host
Rabbit
Species Reactivity
Human
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Concentration
Vial Concentration: 0.43 (lot specific)
Specificity
This BAT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-302 amino acids from the C-terminal region of human BAT4.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Antibody/Peptide Pairs
BAT4 peptide (MBS9227812) is used for blocking the activity of BAT4 antibody (MBS9204019)
Molecular Weight
39314
Immunogen Sequence Positions
275-302
OMIM
142610
3D Structure
ModBase 3D Structure for O95872
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN

Product Categories/Family for anti-GPANK1 antibody

Applications Tested/Suitable for anti-GPANK1 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes for anti-GPANK1 antibody
WB~~1:1000

Western Blot (WB) of anti-GPANK1 antibody

Western blot analysis of BAT4 Antibody (C-term) in mouse spleen tissue lysates (35ug/lane). BAT4 (arrow) was detected using the purified Pab.
Rabbit Anti-GPANK1 Polyclonal Antibody – Cat# MBS9204019

Immunohistochemistry (IHC) of anti-GPANK1 antibody

Formalin-fixed and paraffin-embedded human spleen tissue reacted with BAT4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-GPANK1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS9204019

Flow Cytometry (FC/FACS) of anti-GPANK1 antibody

BAT4 Antibody (C-term) flow cytometry analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-GPANK1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS9204019

Western Blot (WB) of anti-GPANK1 antibody

Western blot analysis of BAT4 (arrow) using rabbit polyclonal BAT4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BAT4 gene.
Rabbit Anti-GPANK1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9204019

Product References and Citations for anti-GPANK1 antibody

Spies,T., et.al., Proc. Natl. Acad. Sci. U.S.A. 86 (22), 8955-8958 (1989)

Other Notes

Small volumes of anti-GPANK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GPANK1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
G patch domain and ankyrin repeat-containing protein 1
NCBI Official Synonym Full Names
G patch domain and ankyrin repeats 1
NCBI Official Symbol
GPANK1
NCBI Official Synonym Symbols
G5; BAT4; D6S54E; ANKRD59; GPATCH10
UniProt Protein Name
G patch domain and ankyrin repeat-containing protein 1
UniProt Synonym Protein Names
Ankyrin repeat domain-containing protein 59; G patch domain-containing protein 10; HLA-B-associated transcript 4; Protein G5
UniProt Gene Name
GPANK1
UniProt Synonym Gene Names
ANKRD59; BAT4; G5; GPATCH10
UniProt Entry Name
GPAN1_HUMAN
NCBI Protein Information
G patch domain and ankyrin repeat-containing protein 1

NCBI Summary for GPANK1

This gene is located in a cluster of HLA-B-associated transcripts, which is included in the human major histocompatability complex III region. This gene encodes a protein which is thought to play a role in immunity. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2010]
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UniProt Comments for GPANK1

BAT4:

Chromosomal Location of Human Ortholog: 6p21.3

Molecular Function: nucleic acid binding

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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