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NMDA NR2C Subunit (Ser1096) (GRIN2C) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502163

MBS502163 is an antibody targeting GRIN2C. Also known as Anti-Phospho Ser1096 NMDA Receptor, NR2C Subunit. Reactive in Mouse, rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

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Product Overview

Online Datasheet in PDF
Product Name
NMDA NR2C Subunit (Ser1096) (GRIN2C), Polyclonal Antibody
Full Product Name
Anti-Phospho Ser1096 NMDA Receptor, NR2C Subunit
Product Gene Name
anti-GRIN2C antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse, rat
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Specificity
Specific for the ~140k NR2C subunit of the NMDA receptor phosphorylated at Ser1096. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to generate the antibody but not by the corresponding dephospho-peptide.
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Molecular Weight
140
Chromosome Location
Chromosome: 10; NC_005109.3 (104479941..104497938). Location: 10q32.2
3D Structure
ModBase 3D Structure for Q00961
Antigen
Synthetic phospho-peptide surrounding the phospho Ser1096 of the NR2C-subunit of rat NMDA receptor.
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1096 conjugated to KLH
Immunogen Species
Rat
Reactivity Assumed Based on 100% Sequence Homology
Bovine, canine, humand and non-human primates
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with mouse and rat tissue. It is also expected that the antibody will react with bovine, canine, human and non-human primates as these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The NR2C subunit of the receptor is thought to influence the NMDAR conductance level (Ebralidze et al., 1996). Phosphorylation of Ser1096 by PKB on NR2C has been recently demonstrated to regulate NMDA receptor binding to 14-3-3 (Chen & Roche 2009).
Applications Tested/Suitable for anti-GRIN2C antibody
Western Blot (WB)
Application Notes for anti-GRIN2C antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000

Testing Data of anti-GRIN2C antibody

Rabbit Anti-GRIN2C Polyclonal Antibody – Cat# MBS502163

Product References and Citations for anti-GRIN2C antibody

• Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025.
• Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.
• Ebralidze AK, Rossi DJ, Tonegawa S, Slater NT (1996) Modification of NMDA receptor channels and synaptic transmission by targeted disruption of the NR2C gene. J Neurosci 16:5014-5025.
• Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
• Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724.

Other Notes

Small volumes of anti-GRIN2C antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GRIN2C. It may not necessarily be applicable to this product.

NCBI GI #
148747563
NCBI GeneID
24411
NCBI Accession #
NP_036707.3
UniProt Primary Accession #
Q00961
UniProt Related Accession #
Q00961
GenBank Nucleotide #
NM_012575.3
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 2C
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, N-methyl D-aspartate 2C
NCBI Official Symbol
Grin2c
NCBI Official Synonym Symbols
NR2C; GluN2C
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 2C
UniProt Synonym Protein Names
Glutamate [NMDA] receptor subunit epsilon-3; N-methyl D-aspartate receptor subtype 2C
Protein Family
Glutamate receptor ionotropic
UniProt Gene Name
Grin2c
UniProt Synonym Gene Names
GluN2C; NMDAR2C; NR2C
UniProt Entry Name
NMDE3_RAT
NCBI Protein Information
glutamate receptor ionotropic, NMDA 2C; NMDAR2C; NMDA glutamate receptor; glutamate receptor, ionotropic, NMDA2C; N-methyl D-aspartate receptor subtype 2C; glutamate [NMDA] receptor subunit epsilon-3

NCBI Summary for GRIN2C

subunit that in combination with NMDAR1 displays NMDA receptor activity; may contribute to NMDA receptor functional diversity [RGD, Feb 2006]

UniProt Comments for GRIN2C

Function: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.

Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Interacts with PDZ domains of INADL and DLG4. Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes. Ref.3 Ref.4

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.

Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2C/GRIN2C subfamily. [View classification]

Sequence caution: The sequence BAA02499.1 differs from that shown. Reason: Erroneous initiation.
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Research Articles on GRIN2C

1. NR2C expression has an initial rise from postnatal day P2 to P3, but remains relatively constant thereafter until P21, except for a significant fall at P12 in the pre-Botzinger complex.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-GRIN2C antibody

 Reference Product  PubMed Publications
 Grin3b antibody  >4 publications with GRIN2C and Grin3b
 Grin1 antibody  >3 publications with GRIN2C and Grin1
 Grin3a antibody  >3 publications with GRIN2C and Grin3a

Pathways associated with anti-GRIN2C antibody

 Products by Pathway  Pathway Diagram
 Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway antibodies  Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies  Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
 CREB Phosphorylation Through The Activation Of CaMKII Pathway antibodies  CREB Phosphorylation Through The Activation Of CaMKII Pathway Diagram

Diseases associated with anti-GRIN2C antibody

 Disease Name  Pubmed Publications
 Hypertrophy Antibodies  >3 publications with GRIN2C and Hypertrophy
 Hepatomegaly Antibodies  >2 publications with GRIN2C and Hepatomegaly
 Liver Diseases Antibodies  >2 publications with GRIN2C and Liver Diseases
 Nervous System Diseases Antibodies  >2 publications with GRIN2C and Nervous System Diseases
 Learning Disorders Antibodies  >2 publications with GRIN2C and Learning Disorders
 Necrosis Antibodies  >2 publications with GRIN2C and Necrosis
 Cardiovascular Diseases Antibodies  >1 publications with GRIN2C and Cardiovascular Diseases
 Hyperplasia Antibodies  >1 publications with GRIN2C and Hyperplasia
 Abnormalities, Drug-Induced Antibodies  >1 publications with GRIN2C and Abnormalities, Drug-Induced
 Brain Diseases Antibodies  >1 publications with GRIN2C and Brain Diseases

Organs/Tissues associated with anti-GRIN2C antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >9 publications with GRIN2C and Brain
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