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NEK1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6489685

MBS6489685 is an antibody targeting NEK1. Also known as NEK1, CT (Never in Mitosis A-related Kinase 1, NimA-related Protein Kinase 1, KIAA1901, MGC138800, NY-REN-55, Renal Carcinoma Antigen NY-REN-55, Serine/threonine Protein Kinase Nek1) (FITC). Reactive in… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NEK1, Polyclonal Antibody
Full Product Name
NEK1, CT (Never in Mitosis A-related Kinase 1, NimA-related Protein Kinase 1, KIAA1901, MGC138800, NY-REN-55, Renal Carcinoma Antigen NY-REN-55, Serine/threonine Protein Kinase Nek1) (FITC)
Product Gene Name
Product Synonym Names
NIMA (Never in Mitosis Gene A)-related Kinase 1; DKFZp686D06121; DKFZp686K12169; EC=2.7.11.1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Hamster
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Purity/Purification
Purified by Protein G Affinity Chromatography.
Specificity
Recognizes human NEK1. Species Crossreactivity: hamster.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
142,828 Da
OMIM
604588
3D Structure
ModBase 3D Structure for Q96PY6
Immunogen
Synthetic peptide selected from the C-terminal region of human NEK1 (KLH).
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-NEK1 antibody

Applications Tested/Suitable for anti-NEK1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-NEK1 antibody
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-NEK1 antibody

The anti-NEK1 Pab is used in Western blot to detect NEK1 in CHO cell lysate.
Rabbit Anti-NEK1 Polyclonal Antibody – Cat# MBS6489685

Immunohistochemistry (IHC) of anti-NEK1 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Rabbit Anti-NEK1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6489685

Product References and Citations for anti-NEK1 antibody

1. Surpili, M.J., et al., Biochemistry 42(51):15369-15376 (2003). 2. Scanlan, M.J., et al., Int. J. Cancer 83(4):456-464 (1999). 3. Letwin, K., et al., EMBO J. 11(10):3521-3531 (1992).

Other Notes

Small volumes of anti-NEK1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEK1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein kinase Nek1 isoform 1
NCBI Official Synonym Full Names
NIMA-related kinase 1
NCBI Official Symbol
NEK1
NCBI Official Synonym Symbols
SRPS2; SRTD6; SRPS2A; NY-REN-55
UniProt Protein Name
Serine/threonine-protein kinase Nek1
UniProt Synonym Protein Names
Never in mitosis A-related kinase 1; NimA-related protein kinase 1; Renal carcinoma antigen NY-REN-55
UniProt Gene Name
NEK1
UniProt Synonym Gene Names
KIAA1901; NimA-related protein kinase 1
UniProt Entry Name
NEK1_HUMAN
NCBI Protein Information
serine/threonine-protein kinase Nek1; nimA-related protein kinase 1; protein-serine/threonine kinase; renal carcinoma antigen NY-REN-55; never in mitosis A-related kinase 1; NIMA (never in mitosis gene a)-related kinase 1

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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