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anti-ACADM antibody :: Goat ACADM Polyclonal Antibody

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Catalog # MBS423554
Unit / Price
  0.1 mg  /  $300 +1 FREE 8GB USB
Western Blot (WB)
Product Name

ACADM, Polyclonal Antibody

Also Known As

Goat anti-ACADM, Biotinylated Antibody

Product Synonym Names
Goat Anti-ACADM Antibody; ACADM; acyl-CoA dehydrogenase, C-4 to C-12 straight chain; ACAD1; MCAD; MCADH; acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain; ACADM antibody; acyl-CoA dehydrogenase; C-4 to C-12 straight chain antibody; ACAD1 antibody; MCAD antibody; MCADH antibody; acyl-Coenzyme A dehydrogenase; C-4 to C-12 straight chain antibody; ACADM, Biotinylated
Matching Pairs
Unconjugated Antibody: ACADM (MBS420143)
Biotin Conjugated Antibody: ACADM (MBS423554)
Antibody/Peptide Pairs
ACADM peptide (MBS426384) is used for blocking the activity of ACADM antibody (MBS423554)
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Immunogen Sequence Length
421
Immunogen Sequence
RLIVAREHID KYKN
OMIM
201450
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (lot specific)
Immunogen
Peptide with sequence C-RLIVAREHIDKYKN. , from the C Terminus of the protein sequence according to NP_000007.1; NP_001120800.1; NP_001272971.1; NP_001272972.1; NP_001272973.1.
Epitope
C Terminus
Note
This antibody is expected to recognise both reported isoforms (NP_000007.1; NP_001120800.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Other Notes
Small volumes of anti-ACADM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Applications Tested/Suitable for anti-ACADM antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-ACADM antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 45kDa band observed in Human Heart lysates (calculated MW of 46.6kDa according to NP_000007.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 2-4ug/ml.

Western Blot (WB) of anti-ACADM antibody
Biotinylated (3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
anti-ACADM antibody Western Blot (WB) (WB) image
NCBI/Uniprot data below describe general gene information for ACADM. It may not necessarily be applicable to this product.
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
Manufactured in an ISO 9001:2015 Certified Laboratory.NP_001120800.1; NP_001272971.1; NP_001272972.1; NP_001272973.1[Other Products]
NCBI GenBank Nucleotide #
UniProt Secondary Accession #
UniProt Related Accession #
Molecular Weight
47,020 Da
NCBI Official Full Name
medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform a
NCBI Official Synonym Full Names
acyl-CoA dehydrogenase, C-4 to C-12 straight chain
NCBI Official Symbol
ACADM  [Similar Products]
NCBI Official Synonym Symbols
MCAD; ACAD1; MCADH
  [Similar Products]
NCBI Protein Information
medium-chain specific acyl-CoA dehydrogenase, mitochondrial
UniProt Protein Name
Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
UniProt Gene Name
ACADM  [Similar Products]
UniProt Synonym Gene Names
UniProt Entry Name
ACADM_HUMAN
NCBI Summary for ACADM
This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Comments for ACADM
ACADM: This enzyme is specific for acyl chain lengths of 4 to 16. Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD). It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy. Belongs to the acyl-CoA dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - valine, leucine and isoleucine degradation; EC 1.3.8.7; Carbohydrate Metabolism - propanoate; Mitochondrial; Other Amino Acids Metabolism - beta-alanine; Oxidoreductase; Lipid Metabolism - fatty acid

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: axon; mitochondrial matrix; mitochondrion; nucleus; peroxisome

Molecular Function: acyl-CoA binding; acyl-CoA dehydrogenase activity; electron carrier activity; FAD binding; identical protein binding

Biological Process: carnitine biosynthetic process; carnitine metabolic process, CoA-linked; fatty acid beta-oxidation; fatty acid beta-oxidation using acyl-CoA dehydrogenase; lipid homeostasis; medium-chain fatty acid catabolic process; medium-chain fatty acid metabolic process

Disease: Acyl-coa Dehydrogenase, Medium-chain, Deficiency Of
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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