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ADRBK2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6271937

ADRBK2 is the beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta… More details

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Testing Data View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ADRBK2, Polyclonal Antibody
Full Product Name
ADRBK2, NT (ADRBK2, BARK2, GRK3, Beta-adrenergic receptor kinase 2, G-protein-coupled receptor kinase 3) (AP)
Product Gene Name
Product Synonym Names
ADRBK2; BARK2; GRK3; Beta-adrenergic receptor kinase 2; G-protein-coupled receptor kinase 3
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Purity/Purification
Purified by Protein A Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
79,710 Da
Chromosome Location
Chromosome: 22; NC_000022.10 (25960861..26125259). Location: 22q12.1
OMIM
109636
3D Structure
ModBase 3D Structure for P35626
Immunogen
ADRBK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36~65 amino acids from the N-terminal region of human ADRBK2.
Conjugate
AP
Note
Preservative Free

Product Categories/Family for anti-ADRBK2 antibody

Applications Tested/Suitable for anti-ADRBK2 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-ADRBK2 antibody
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ADRBK2 antibody

Western blot analysis of ADRBK2 (arrow) using rabbit polyclonal ADRBK2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ADRBK2 gene (Lane 2).
Rabbit Anti-ADRBK2 Polyclonal Antibody – Cat# MBS6271937

Immunohistochemistry (IHC) of anti-ADRBK2 antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with ADRBK2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-ADRBK2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6271937

Product References and Citations for anti-ADRBK2 antibody

Gratacos, M., et.al., Am. J. Med. Genet. B Neuropsychiatr. Genet. 150B (6), 808-816 (2009). Rao, J.S., et.al., Int. J. Neuropsychopharmacol. 12 (6), 851-860 (2009).

Other Notes

Small volumes of anti-ADRBK2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ADRBK2 purchase

MBS6271937 is an antibody targeting ADRBK2. Also known as ADRBK2, NT (ADRBK2, BARK2, GRK3, Beta-adrenergic receptor kinase 2, G-protein-coupled receptor kinase 3) (AP). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ADRBK2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
beta-adrenergic receptor kinase 2
NCBI Official Synonym Full Names
adrenergic, beta, receptor kinase 2
NCBI Official Symbol
ADRBK2
NCBI Official Synonym Symbols
GRK3; BARK2
UniProt Protein Name
Beta-adrenergic receptor kinase 2
UniProt Synonym Protein Names
G-protein-coupled receptor kinase 3
UniProt Gene Name
ADRBK2
UniProt Synonym Gene Names
BARK2; GRK3; Beta-ARK-2
UniProt Entry Name
ARBK2_HUMAN
NCBI Protein Information
beta-adrenergic receptor kinase 2; beta-ARK-2; G-protein-coupled receptor kinase 3

NCBI Summary for ADRBK2

The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008]
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UniProt Comments for ADRBK2

GRK3: Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.

Protein type: Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.15; AGC group; GRK family; BARK subfamily

Chromosomal Location of Human Ortholog: 22q12.1

Molecular Function: G-protein coupled receptor kinase activity; beta-adrenergic receptor kinase activity; ATP binding; protein kinase activity

Biological Process: receptor internalization; signal transduction; protein amino acid phosphorylation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ADRBK2 antibody

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