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AGR2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6463285

MBS6463285 is an antibody targeting AGR2. Also known as AGR2, NT (Anterior Gradient Protein 2 Homolog, Secreted Cement Gland Protein XAG-2 Homolog, AG-2, hAG-2, HPC8, AG2, Anterior Gradient 2, UNQ515/PRO1030) (AP). Reactive in Human. Host: Rabbit. More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AGR2, Polyclonal Antibody
Full Product Name
AGR2, NT (Anterior Gradient Protein 2 Homolog, Secreted Cement Gland Protein XAG-2 Homolog, AG-2, hAG-2, HPC8, AG2, Anterior Gradient 2, UNQ515/PRO1030) (AP)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human AGR2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
19 kDa
OMIM
606358
3D Structure
ModBase 3D Structure for O95994
Immunogen
Synthetic peptide selected from the N-terminal region of human AGR2 (KLH).
Conjugate
AP
Note
Preservative Free

Product Categories/Family for anti-AGR2 antibody

Applications Tested/Suitable for anti-AGR2 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes for anti-AGR2 antibody
IHC: 1:10-1:50
ELISA: 1:1,000
WB: 1:50-1:100
Applications are based on unconjugated antibody.

Immunofluorescence (IF) of anti-AGR2 antibody

Fluorescent confocal image of A549 cell stained with AGR2 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.
Rabbit Anti-AGR2 Polyclonal Antibody – Cat# MBS6463285

Western Blot (WB) of anti-AGR2 antibody

Western blot analysis of AGR2 Antibody (N-term) in T47D cell line lysates (35ug/lane). AGR2 (arrow) was detected using the purified Pab.
Rabbit Anti-AGR2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6463285

Western Blot (WB) of anti-AGR2 antibody

Western blot analysis of AGR2(arrow) using rabbit polyclonal AGR2 Antibody (N-term).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AGR2 gene (Lane 2) (Origene Technologies).
Rabbit Anti-AGR2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6463285

Immunohistochemistry (IHC) of anti-AGR2 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-AGR2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6463285

Product References and Citations for anti-AGR2 antibody

1. Zweitzig, D.R., Mol. Cell. Biochem. 306(1-2), 255-260 (2007). 2. Zhang, Y., Prostate Cancer Prostatic Dis. 10(3), 293-300 (2007). 3. Fletcher, G.C., Br. J. Cancer 88(4), 579-585 (2003).

Other Notes

Small volumes of anti-AGR2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AGR2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
anterior gradient protein 2 homolog
NCBI Official Synonym Full Names
anterior gradient 2
NCBI Official Symbol
AGR2
NCBI Official Synonym Symbols
AG2; GOB-4; HAG-2; XAG-2; PDIA17; HEL-S-116
UniProt Protein Name
Anterior gradient protein 2 homolog
UniProt Synonym Protein Names
HPC8; Secreted cement gland protein XAG-2 homolog
UniProt Gene Name
AGR2
UniProt Synonym Gene Names
AG2; AG-2; hAG-2
UniProt Entry Name
AGR2_HUMAN
NCBI Protein Information
anterior gradient protein 2 homolog; AG-2; HPC8; anterior gradient homolog 2; secreted cement gland homolog; epididymis secretory protein Li 116; secreted cement gland protein XAG-2 homolog; protein disulfide isomerase family A, member 17

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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