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ALDH1A1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6462280

MBS6462280 is an antibody targeting ALDH1A1. Also known as ALDH1A1, ID (Retinal Dehydrogenase 1, RalDH1, RALDH 1, Aldehyde Dehydrogenase Family 1 Member A1, Aldehyde Dehydrogenase, Cytosolic, ALHDII, ALDH-E1, ALDH1A1, ALDC, ALDH1, PUMB1) (PE). Reactive in… More details

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Product Overview

Online Datasheet in PDF
Product Name
ALDH1A1, Polyclonal Antibody
Full Product Name
ALDH1A1, ID (Retinal Dehydrogenase 1, RalDH1, RALDH 1, Aldehyde Dehydrogenase Family 1 Member A1, Aldehyde Dehydrogenase, Cytosolic, ALHDII, ALDH-E1, ALDH1A1, ALDC, ALDH1, PUMB1) (PE)
Product Gene Name
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Specificity
Recognizes human ALDH1A1.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
54.8 kDa (501aa), confirmed by MALDI-TOF.
OMIM
100640
3D Structure
ModBase 3D Structure for P00352
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 302-331 in the central region of human ALDH1A1, UniProt Accession #P00352. Species sequence homology: monkey, mouse
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive

Product Categories/Family for anti-ALDH1A1 antibody

Applications Tested/Suitable for anti-ALDH1A1 antibody
Immunofluorescence (IF), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for anti-ALDH1A1 antibody
IF: 1:10-1:50
FLISA: 1:1,000
IHC: 1:50
WB: 1:50-1:100
Applications are based on unconjugated antibody.

Western Blot (WB) of anti-ALDH1A1 antibody

Western blot analysis of ALDH1A1 (arrow) usingMBS627327. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (Lane 2) (Origene Technologies).
Rabbit Anti-ALDH1A1 Polyclonal Antibody – Cat# MBS6462280

Immunohistochemistry (IHC) of anti-ALDH1A1 antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted usingMBS627327, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Rabbit Anti-ALDH1A1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6462280

Immunofluorescence (IF) of anti-ALDH1A1 antibody

Immunofluorescence analysis of in HeLa cells usingMBS627327. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Rabbit Anti-ALDH1A1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS6462280

Flow Cytometry (FC/FACS) of anti-ALDH1A1 antibody

Flow cytometric analysis of HepG2 cells using MBS627327 (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ALDH1A1 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6462280

Product References and Citations for anti-ALDH1A1 antibody

1. Moore, S., J Stud Alcohol Drugs 68(2), 192-196 (2007). 2. Collard, F., Biochimie 89(3), 369-373 (2007).

Other Notes

Small volumes of anti-ALDH1A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ALDH1A1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
retinal dehydrogenase 1
NCBI Official Synonym Full Names
aldehyde dehydrogenase 1 family member A1
NCBI Official Symbol
ALDH1A1
NCBI Official Synonym Symbols
ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
UniProt Protein Name
Retinal dehydrogenase 1
UniProt Synonym Protein Names
ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic
Protein Family
UniProt Gene Name
ALDH1A1
UniProt Synonym Gene Names
ALDC; ALDH1; PUMB1; RALDH 1; RalDH1
UniProt Entry Name
AL1A1_HUMAN
NCBI Protein Information
retinal dehydrogenase 1

NCBI Summary for ALDH1A1

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]
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UniProt Comments for ALDH1A1

ALDH1A1: Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Belongs to the aldehyde dehydrogenase family.

Protein type: GAPs, Ras; EC 1.2.1.36; GAPs; Cofactor and Vitamin Metabolism - retinol; Apoptosis; Oxidoreductase

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: cytoplasm; cytosol

Molecular Function: aldehyde dehydrogenase (NAD) activity; androgen binding; 3-chloroallyl aldehyde dehydrogenase activity; benzaldehyde dehydrogenase (NAD+) activity; retinal dehydrogenase activity

Biological Process: retinol metabolic process; xenobiotic metabolic process; aldehyde metabolic process; ethanol oxidation
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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