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ALKBH8 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6462510

MBS6462510 is an antibody targeting ALKBH8. Also known as ALKBH8, CT (Alkylated DNA Repair Protein alkB Homolog 8, Probable alpha-ketoglutarate-dependent Dioxygenase ABH8, S-adenosyl-L-methionine-dependent tRNA Methyltransferase ABH8, ABH8) (MaxLight 750).… More details

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Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ALKBH8, Polyclonal Antibody
Full Product Name
ALKBH8, CT (Alkylated DNA Repair Protein alkB Homolog 8, Probable alpha-ketoglutarate-dependent Dioxygenase ABH8, S-adenosyl-L-methionine-dependent tRNA Methyltransferase ABH8, ABH8) (MaxLight 750)
Product Gene Name
anti-ALKBH8 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human ALKBH8.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
26kDa
OMIM
613306
3D Structure
ModBase 3D Structure for Q96BT7
Immunogen
Synthetic peptide selected from the C-terminal region of human ALKBH8 (KLH).
Conjugate
MaxLight750
Note
Preservative Free

Product Categories/Family for anti-ALKBH8 antibody

Antibodies; DNA Replication; Repair
Applications Tested/Suitable for anti-ALKBH8 antibody
Western Blot (WB), FLISA
Application Notes for anti-ALKBH8 antibody
WB: 1:50-1:100
FLISA: 1:1,000
Applications are based on unconjugated antibody.

Immunohistochemistry (IHC) of anti-ALKBH8 antibody

Formalin-fixed and paraffin-embedded human spleen reacted with ALKBH8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-ALKBH8 Polyclonal Antibody – Cat# MBS6462510

Western Blot (WB) of anti-ALKBH8 antibody

Western blot analysis of ALKBH8 Antibody (C-term) in HL60 cell line lysates (35ug/lane). ALKBH8 (arrow) was detected using the purified Pab.
Rabbit Anti-ALKBH8 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6462510

Flow Cytometry (FC/FACS) of anti-ALKBH8 antibody

Flow cytometric analysis of NCI-H292 cells using ALKBH8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ALKBH8 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6462510

Product References and Citations for anti-ALKBH8 antibody

1. Shimada, K., et.al., Cancer Res. 69(7), 3157-3164 (2009).

Other Notes

Small volumes of anti-ALKBH8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ALKBH8. It may not necessarily be applicable to this product.

NCBI GI #
195927056
NCBI GeneID
91801
NCBI Accession #
NP_620130
UniProt Primary Accession #
Q96BT7
UniProt Related Accession #
Q96BT7
GenBank Nucleotide #
NM_138775
NCBI Official Full Name
alkylated DNA repair protein alkB homolog 8 isoform 2
NCBI Official Synonym Full Names
alkB homolog 8, tRNA methyltransferase
NCBI Official Symbol
ALKBH8
NCBI Official Synonym Symbols
ABH8; TRM9; TRMT9; TRMT9A
UniProt Protein Name
Alkylated DNA repair protein alkB homolog 8
UniProt Synonym Protein Names
Probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8
Protein Family
Alkylated DNA repair protein
UniProt Gene Name
ALKBH8
UniProt Synonym Gene Names
ABH8
UniProt Entry Name
ALKB8_HUMAN
NCBI Protein Information
alkylated DNA repair protein alkB homolog 8

UniProt Comments for ALKBH8

ALKBH8: Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis. Belongs to the alkB family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.229; Methyltransferase; RNA processing

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: microtubule cytoskeleton; nucleoplasm; nucleus; cytosol

Molecular Function: protein binding; zinc ion binding; tRNA (uracil) methyltransferase activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors; tRNA binding

Biological Process: tRNA wobble uridine modification; response to DNA damage stimulus; tRNA methylation
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Research Articles on ALKBH8

1. findings suggest that the high expression of ALKBH8 is critical for the growth and progression of bladder cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Diseases associated with anti-ALKBH8 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1 publications with ALKBH8 and Neoplasms
 Carcinoma Antibodies  >1 publications with ALKBH8 and Carcinoma

Organs/Tissues associated with anti-ALKBH8 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1 publications with ALKBH8 and Blood
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