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AMH Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1750481

Description: Anti-Mllerian hormone (AMH), also known as MIF or MIS, is a protein that in humans is encoded by the AMH gene. It is a hormone that inhibits the development of the Mllerian ducts (paramesonephric ducts) in the male embryo. Expression of AMH is… More details

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Product Overview

Online Datasheet in PDF
Product Name
AMH/Mis, Polyclonal Antibody
Full Product Name
Anti-AMH/Mis Picoband Antibody
Product Gene Name
anti-AMH antibody
Product Synonym Names
Muellerian-inhibiting factor; Anti-Muellerian hormone; AMH; Muellerian-inhibiting substance; MIS; AMH; MIF
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
No cross reactivity with other proteins.
Form/Format
Lyophilized
Purity/Purification
Immunogen affinity purified
Concentration
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. (lot specific)
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Molecular Weight
59195 MW
OMIM
K03474 Genomic DNA
3D Structure
ModBase 3D Structure for P03971
Immunogen
E Coli-derived human AMH recombinant protein (Position: A75-E141). Human AMH shares 66.7% amino acid (aa) sequence identity with both mouse and rat AMH.
Subcellular Localization
Secreted.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Product Categories/Family for anti-AMH antibody

Signal Transduction; Growth Factors/Hormones; TGF; Stem Cells; Signaling Pathways; TGF Beta; Secreted; Developmental Biology; Reproduction; Sex Determination; Placental Development
ISO Certification
Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.
Applications Tested/Suitable for anti-AMH antibody
Western Blot (WB)
Application Notes for anti-AMH antibody
WB: 0.1-0.5mug/ml

Western Blot (WB) of anti-AMH antibody

Figure 1. Western blot analysis of AMH using anti- AMH antibody (MBS1750481).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: 293T whole Cell lysates,
Lane 2: COLO320 whole Cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- AMH antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AMH4 at approximately 65KD. The expected band size for AMH is at 59KD.
Rabbit Anti-AMH Polyclonal Antibody – Cat# MBS1750481

Other Notes

Small volumes of anti-AMH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for AMH purchase

MBS1750481 is an antibody targeting AMH. Also known as Anti-AMH/Mis Picoband Antibody. Reactive in Human
No cross reactivity with other proteins.. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for AMH. It may not necessarily be applicable to this product.

NCBI GI #
157266298
NCBI GeneID
268
NCBI Accession #
NP_000470.2
UniProt Primary Accession #
P03971
UniProt Secondary Accession #
O75246; Q6GTN3
UniProt Related Accession #
P03971
GenBank Nucleotide #
NM_000479.4
NCBI Official Full Name
muellerian-inhibiting factor preproprotein
NCBI Official Synonym Full Names
anti-Mullerian hormone
NCBI Official Symbol
AMH
NCBI Official Synonym Symbols
MIF; MIS
UniProt Protein Name
Muellerian-inhibiting factor
UniProt Synonym Protein Names
Anti-Muellerian hormone; AMH; Muellerian-inhibiting substance; MIS
Protein Family
Muellerian-inhibiting factor
UniProt Gene Name
AMH
UniProt Synonym Gene Names
MIF; AMH; MIS
NCBI Protein Information
muellerian-inhibiting factor

NCBI Summary for AMH

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate N- and C-terminal cleavage products that homodimerize and associate to form a biologically active noncovalent complex. This complex binds to the anti-Mullerian hormone receptor type 2 and causes the regression of Mullerian ducts in the male embryo that would otherwise differentiate into the uterus and fallopian tubes. This protein also plays a role in Leydig cell differentiation and function and follicular development in adult females. Mutations in this gene result in persistent Mullerian duct syndrome. [provided by RefSeq, Jul 2016]
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UniProt Comments for AMH

This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.

Research Articles on AMH

1. Women with unexplained infertility did not show evidence of decreased ovarian reserve as measured by AMH and antral follicle count.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-AMH antibody

 Reference Product  PubMed Publications
 FSH antibody  >545 publications with AMH and FSH
 SOX9 antibody  >75 publications with AMH and SOX9
 AMHR2 antibody  >32 publications with AMH and AMHR2
 GATA4 antibody  >21 publications with AMH and GATA4
 KITLG antibody  >13 publications with AMH and KITLG
 ZFPM2 antibody  >3 publications with AMH and ZFPM2
 SMAD5 antibody  >3 publications with AMH and SMAD5
 SMAD9 antibody  >1 publications with AMH and SMAD9
 ACVR1 antibody  >1 publications with AMH and ACVR1

Pathways associated with anti-AMH antibody

 Products by Pathway  Pathway Diagram
 ALK2 Signaling Events Pathway antibodies  ALK2 Signaling Events Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
 TGF-beta Signaling Pathway antibodies  TGF-beta Signaling Pathway Diagram
 CAMP Signaling Pathway antibodies  CAMP Signaling Pathway Diagram
 CAMP Signaling Pathway antibodies  CAMP Signaling Pathway Diagram

Diseases associated with anti-AMH antibody

 Disease Name  Pubmed Publications
 Female Urogenital Diseases Antibodies  >549 publications with AMH and Female Urogenital Diseases
 Gonadal Disorders Antibodies  >449 publications with AMH and Gonadal Disorders
 Neoplasms Antibodies  >309 publications with AMH and Neoplasms
 Ovarian Diseases Antibodies  >306 publications with AMH and Ovarian Diseases
 Infertility, Female Antibodies  >264 publications with AMH and Infertility, Female
 Urogenital Abnormalities Antibodies  >151 publications with AMH and Urogenital Abnormalities
 Genital Diseases, Male Antibodies  >129 publications with AMH and Genital Diseases, Male
 Disorders of Sex Development Antibodies  >129 publications with AMH and Disorders of Sex Development
 Infertility, Male Antibodies  >76 publications with AMH and Infertility, Male
 Testicular Diseases Antibodies  >65 publications with AMH and Testicular Diseases

Organs/Tissues associated with anti-AMH antibody

 Organ/Tissue Name  Pubmed Publications
 Testis Antibodies  >319 publications with AMH and Testis
 Uterus Antibodies  >66 publications with AMH and Uterus
 Brain Antibodies  >50 publications with AMH and Brain
 Embryonic Tissue Antibodies  >47 publications with AMH and Embryonic Tissue
 Lung Antibodies  >19 publications with AMH and Lung
 Prostate Antibodies  >12 publications with AMH and Prostate
 Intestine Antibodies  >9 publications with AMH and Intestine
 Eye Antibodies  >4 publications with AMH and Eye
 Stomach Antibodies  >2 publications with AMH and Stomach
 Mammary Gland Antibodies  >2 publications with AMH and Mammary Gland
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