Rabbit Anti-ANAPC2 Polyclonal Antibody – Cat# MBS9128682

Western blot analysis of extracts of various cell lines, using ANAPC2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

ANAPC2 Antibody – Rabbit Polyclonal

Name: ANAPC2 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS9128682
Price: 225 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9128682

A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

ANAPC2, Polyclonal Antibody

Full Product Name

ANAPC2 Polyclonal Antibody

Product Gene Name

anti-ANAPC2 antibody

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Purity/Purification

Affinity Purification

Preparation and Storage

Store at -20 degree C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Molecular Weight

94kDa

OMIM

606946

3D Structure

ModBase 3D Structure for Q9UJX6

Immunogen

Recombinant protein of human ANAPC2

ISO Certification

Manufactured in an ISO 9001:2015 Certified Laboratory.

Applications Tested/Suitable for anti-ANAPC2 antibody

Western Blot (WB)

Application Notes for anti-ANAPC2 antibody

WB 1:500 - 1:2000

Other Notes

Small volumes of anti-ANAPC2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ANAPC2 purchase

MBS9128682 is an antibody targeting ANAPC2. Also known as ANAPC2 Polyclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ANAPC2. It may not necessarily be applicable to this product.

NCBI GI #

7019327

NCBI GeneID

29882

NCBI Accession #

NP_037498.1

UniProt Primary Accession #

Q9UJX6

UniProt Secondary Accession #

Q5VSG1; Q96DG5; Q96GG4; Q9P2E1

UniProt Related Accession #

Q9UJX6

GenBank Nucleotide #

NM_013366.3

NCBI Official Full Name

anaphase-promoting complex subunit 2

NCBI Official Synonym Full Names

anaphase promoting complex subunit 2

NCBI Official Symbol

ANAPC2

NCBI Official Synonym Symbols

APC2

UniProt Protein Name

Anaphase-promoting complex subunit 2

UniProt Synonym Protein Names

Cyclosome subunit 2

Protein Family

Anaphase-promoting complex

UniProt Gene Name

ANAPC2

UniProt Synonym Gene Names

APC2; KIAA1406; APC2

UniProt Entry Name

ANC2_HUMAN

NCBI Protein Information

anaphase-promoting complex subunit 2

UniProt Comments for ANAPC2

APC2: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. Belongs to the cullin family. The APC/C is composed of at least 12 subunits. Interacts through the cullin domain with ANAPC11 and with UBCH10. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: nucleoplasm; anaphase-promoting complex; cytosol

Molecular Function: ubiquitin protein ligase binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; positive regulation of axon extension; mitotic cell cycle spindle assembly checkpoint; positive regulation of synaptic plasticity; positive regulation of dendrite morphogenesis; mitotic cell cycle

Research Articles on ANAPC2

1. The regulation of Mdm2 by the E3 ubiquitin ligase APC/C is shown. It has important therapeutic implications for tumors with Mdm2 overexpression.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ANAPC2 antibody

Reference Product	PubMed Publications
ANAPC1 antibody	>10 publications with ANAPC2 and ANAPC1
CDC20 antibody	>8 publications with ANAPC2 and CDC20
CDC23 antibody	>8 publications with ANAPC2 and CDC23
CDC27 antibody	>7 publications with ANAPC2 and CDC27
ANAPC10 antibody	>6 publications with ANAPC2 and ANAPC10
CDC16 antibody	>6 publications with ANAPC2 and CDC16
ANAPC4 antibody	>5 publications with ANAPC2 and ANAPC4
ANAPC5 antibody	>4 publications with ANAPC2 and ANAPC5
ANAPC7 antibody	>2 publications with ANAPC2 and ANAPC7

Pathways associated with anti-ANAPC2 antibody

Products by Pathway	Pathway Diagram
APC-Cdc20 Mediated Degradation Of Nek2A Pathway antibodies	APC-Cdc20 Mediated Degradation Of Nek2A Pathway Diagram
APC/C Complex Pathway antibodies	APC/C Complex Pathway Diagram
APC/C Complex Pathway antibodies	APC/C Complex Pathway Diagram
APC/C-mediated Degradation Of Cell Cycle Proteins Pathway antibodies	APC/C-mediated Degradation Of Cell Cycle Proteins Pathway Diagram
APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway antibodies	APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway Diagram
APC/C:Cdc20 Mediated Degradation Of Securin Pathway antibodies	APC/C:Cdc20 Mediated Degradation Of Securin Pathway Diagram
APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies	APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram
APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway antibodies	APC/C:Cdh1 Mediated Degradation Of Cdc20 And Other APC/C:Cdh1 Targeted Proteins In Late Mitosis/early G1 Pathway Diagram
APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway antibodies	APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway Diagram
Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway antibodies	Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway Diagram

Diseases associated with anti-ANAPC2 antibody

Disease Name	Pubmed Publications
Kidney Diseases Antibodies	>2 publications with ANAPC2 and Kidney Diseases
Uterine Neoplasms Antibodies	>1 publications with ANAPC2 and Uterine Neoplasms
Breast Neoplasms Antibodies	>1 publications with ANAPC2 and Breast Neoplasms
Urologic Neoplasms Antibodies	>1 publications with ANAPC2 and Urologic Neoplasms
Kidney Neoplasms Antibodies	>1 publications with ANAPC2 and Kidney Neoplasms
Memory Disorders Antibodies	>1 publications with ANAPC2 and Memory Disorders
Infertility, Male Antibodies	>1 publications with ANAPC2 and Infertility, Male
Learning Disorders Antibodies	>1 publications with ANAPC2 and Learning Disorders
Uterine Cervical Neoplasms Antibodies	>1 publications with ANAPC2 and Uterine Cervical Neoplasms

Organs/Tissues associated with anti-ANAPC2 antibody

Organ/Tissue Name	Pubmed Publications
Kidney Antibodies	>2 publications with ANAPC2 and Kidney
Liver Antibodies	>2 publications with ANAPC2 and Liver
Ovary Antibodies	>1 publications with ANAPC2 and Ovary
Muscle Antibodies	>1 publications with ANAPC2 and Muscle
Pancreas Antibodies	>1 publications with ANAPC2 and Pancreas
Eye Antibodies	>1 publications with ANAPC2 and Eye
Skin Antibodies	>1 publications with ANAPC2 and Skin
Blood Antibodies	>1 publications with ANAPC2 and Blood
Embryonic Tissue Antibodies	>1 publications with ANAPC2 and Embryonic Tissue
Nerve Antibodies	>1 publications with ANAPC2 and Nerve