Rabbit Anti-TNFSF13 Polyclonal Antibody – Cat# MBS1755086

Figure 1. Western blot analysis of APRIL/TNFSF13 using anti-APRIL/TNFSF13 antibody (MBS1755086).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.

Lane 1: human U-937 whole cell lysates.

After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APRIL/TNFSF13 antigen affinity purified polyclonal antibody (#MBS1755086) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for APRIL/TNFSF13 at approximately 32 kDa. The expected band size for APRIL/TNFSF13 is at 27 kDa.

APRIL/TNFSF13 (TNFSF13) Antibody – Rabbit Polyclonal

Name: TNFSF13 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS1755086
Price: 210 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS1755086

A proliferation-inducing ligand (APRIL), also known as tumor necrosis factor ligand superfamily member 13 (TNFSF13), is a protein of the TNF superfamily recognized by the cell surface receptor TACI. The protein encoded by this gene is a member of the tumor… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

APRIL/TNFSF13, Polyclonal Antibody

Full Product Name

Anti-APRIL/TNFSF13 Antibody Picoband

Product Gene Name

anti-TNFSF13 antibody

Clonality

Polyclonal

Isotype

Rabbit IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purity/Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (lot specific)

Preparation and Storage

Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.

Molecular Weight

27,433 Da

Chromosome Location

Chromosome: 17; NC_000017.10 (7461609..7464925). Location: 17p13.1

OMIM

604472

3D Structure

ModBase 3D Structure for O75888

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.

Immunogen

E Coli-derived human APRIL/TNFSF13 recombinant protein (Position: M1-L250).

Product Categories/Family for anti-TNFSF13 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Applications Tested/Suitable for anti-TNFSF13 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-TNFSF13 antibody

WB: 0.25-0.5 ug/ml, Human
Direct ELISA: 0.1-0.5 ug/ml, Human
Tested Species: In-house tested species with positive results.
Enhanced Chemiluminescent Kit with anti-Rabbit IgG (#MBS176460) for Western blot.

Other Notes

Small volumes of anti-TNFSF13 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TNFSF13 purchase

MBS1755086 is an antibody targeting TNFSF13. Also known as Anti-APRIL/TNFSF13 Antibody Picoband. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for TNFSF13. It may not necessarily be applicable to this product.

NCBI GI #

310750387

NCBI GeneID

8741

NCBI Accession #

NP_001185552.1

UniProt Primary Accession #

O75888

UniProt Secondary Accession #

Q541E1; Q5U0G8; Q96HV6; Q9P1M8; Q9P1M9; B4DVT2

UniProt Related Accession #

O75888

GenBank Nucleotide #

NM_001198623.1

NCBI Official Full Name

tumor necrosis factor ligand superfamily member 13 isoform zeta

NCBI Official Synonym Full Names

tumor necrosis factor (ligand) superfamily, member 13

NCBI Official Symbol

TNFSF13

NCBI Official Synonym Symbols

APRIL; CD256; TALL2; ZTNF2; TALL-2; TRDL-1; UNQ383/PRO715

UniProt Protein Name

Tumor necrosis factor ligand superfamily member 13

UniProt Synonym Protein Names

A proliferation-inducing ligand; APRIL; TNF- and APOL-related leukocyte expressed ligand 2; TALL-2; TNF-related death ligand 1

Protein Family

Tumor necrosis factor ligand superfamily

UniProt Gene Name

TNFSF13

UniProt Synonym Gene Names

APRIL; TALL2; ZTNF2; APRIL; TALL-2; TRDL-1

UniProt Entry Name

TNF13_HUMAN

NCBI Protein Information

tumor necrosis factor ligand superfamily member 13; a proliferation-inducing ligand; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; TNF- and APOL-related leukocyte expressed ligand 2

NCBI Summary for TNFSF13

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Alternative splicing results in multiple transcript variants. Some transcripts that skip the last exon of the upstream gene (TNFSF12) and continue into the second exon of this gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]

UniProt Comments for TNFSF13

TNFSF13: Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. Belongs to the tumor necrosis factor family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: nucleoplasm; extracellular space; cytoplasm; cytosol; external side of plasma membrane

Molecular Function: cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: immunoglobulin production in mucosal tissue; positive regulation of cell proliferation; positive regulation of germinal center formation; gene expression; signal transduction; positive regulation of isotype switching to IgA isotypes

Research Articles on TNFSF13

1. Serum APRIL levels inversely correlate with serum IgG4 levels in patients with IgG4-related disease.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-TNFSF13 antibody

Reference Product	PubMed Publications
TNFSF13B antibody	>89 publications with TNFSF13 and TNFSF13B
TNFRSF17 antibody	>55 publications with TNFSF13 and TNFRSF17
TNFRSF13B antibody	>43 publications with TNFSF13 and TNFRSF13B
FURIN antibody	>5 publications with TNFSF13 and FURIN
TNFRSF11B antibody	>2 publications with TNFSF13 and TNFRSF11B
FAS antibody	>2 publications with TNFSF13 and FAS

Pathways associated with anti-TNFSF13 antibody

Products by Pathway	Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Cytokine-cytokine Receptor Interaction Pathway antibodies	Cytokine-cytokine Receptor Interaction Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
Intestinal Immune Network For IgA Production Pathway antibodies	Intestinal Immune Network For IgA Production Pathway Diagram
Intestinal Immune Network For IgA Production Pathway antibodies	Intestinal Immune Network For IgA Production Pathway Diagram
Regulation Of MRNA Stability By Proteins That Bind AU-rich Elements Pathway antibodies	Regulation Of MRNA Stability By Proteins That Bind AU-rich Elements Pathway Diagram
Rheumatoid Arthritis Pathway antibodies	Rheumatoid Arthritis Pathway Diagram
Rheumatoid Arthritis Pathway antibodies	Rheumatoid Arthritis Pathway Diagram
Spinal Cord Injury Pathway antibodies	Spinal Cord Injury Pathway Diagram
Stabilization Of MRNA By HuR Pathway antibodies	Stabilization Of MRNA By HuR Pathway Diagram

Diseases associated with anti-TNFSF13 antibody

Disease Name	Pubmed Publications
Necrosis Antibodies	>191 publications with TNFSF13 and Necrosis
Neoplasms Antibodies	>64 publications with TNFSF13 and Neoplasms
Inflammation Antibodies	>20 publications with TNFSF13 and Inflammation
Disease Models, Animal Antibodies	>12 publications with TNFSF13 and Disease Models, Animal
Cardiovascular Diseases Antibodies	>11 publications with TNFSF13 and Cardiovascular Diseases
Multiple Myeloma Antibodies	>10 publications with TNFSF13 and Multiple Myeloma
Nervous System Diseases Antibodies	>7 publications with TNFSF13 and Nervous System Diseases
Colorectal Neoplasms Antibodies	>6 publications with TNFSF13 and Colorectal Neoplasms
Neoplasms, Experimental Antibodies	>5 publications with TNFSF13 and Neoplasms, Experimental
Kidney Diseases Antibodies	>4 publications with TNFSF13 and Kidney Diseases

Organs/Tissues associated with anti-TNFSF13 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>148 publications with TNFSF13 and Blood
Bone Antibodies	>17 publications with TNFSF13 and Bone
Spleen Antibodies	>15 publications with TNFSF13 and Spleen
Lung Antibodies	>9 publications with TNFSF13 and Lung
Kidney Antibodies	>8 publications with TNFSF13 and Kidney
Lymph Node Antibodies	>8 publications with TNFSF13 and Lymph Node
Skin Antibodies	>6 publications with TNFSF13 and Skin
Vascular Antibodies	>5 publications with TNFSF13 and Vascular
Liver Antibodies	>4 publications with TNFSF13 and Liver
Thymus Antibodies	>3 publications with TNFSF13 and Thymus