APRT Antibody – Rabbit Polyclonal

Figure 1. Western blot analysis of APRT using anti-APRT antibody (MBS178668).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APRT antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for APRT at approximately 20KD. The expected band size for APRT is at 20KD.

Figure 2. Flow Cytometry analysis of A549 cells using anti-APRT antibody (MBS178668).
Overlay histogram showing A549 cells stained with MBS178668 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APRT Antibody (MBS178668,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Figure 3. Flow Cytometry analysis of PC-3 cells using anti-APRT antibody (MBS178668).
Overlay histogram showing PC-3 cells stained with MBS178668 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-APRT Antibody (MBS178668,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.