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ARGBP2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6463921

MBS6463921 is an antibody targeting ARGBP2. Also known as ARGBP2, NT (Sorbin and SH3 Domain-containing Protein 2, Arg/Abl-interacting Protein 2, ArgBP2, Sorbin, KIAA0777, SORBS2) (PE). Reactive in Human. Host: Rabbit. More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ARGBP2, Polyclonal Antibody
Full Product Name
ARGBP2, NT (Sorbin and SH3 Domain-containing Protein 2, Arg/Abl-interacting Protein 2, ArgBP2, Sorbin, KIAA0777, SORBS2) (PE)
Product Gene Name
anti-ARGBP2 antibody
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Specificity
Recognizes human ARGBP2.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight
124.108
3D Structure
ModBase 3D Structure for O94875
Immunogen
Synthetic peptide selected from the N-terminal region of human ARGBP2 (KLH).
Conjugate
PE
Note
Preservative Free

Product Categories/Family for anti-ARGBP2 antibody

Antibodies; Tyrosine Kinases
Applications Tested/Suitable for anti-ARGBP2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes for anti-ARGBP2 antibody
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Immunofluorescence (IF) of anti-ARGBP2 antibody

Fluorescent image of U251 cell stained with ARGBP2 Antibody (N-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARGBP2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).ARGBP2 immunoreactivity is localized to Nucleus significantly.
Rabbit Anti-ARGBP2 Polyclonal Antibody – Cat# MBS6463921

Western Blot (WB) of anti-ARGBP2 antibody

Western blot analysis of ARGBP2 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). ARGBP2 (arrow) was detected using the purified Pab.
Rabbit Anti-ARGBP2 Polyclonal Antibody, Western Blot (WB) – Cat# MBS6463921

Immunohistochemistry (IHC) of anti-ARGBP2 antibody

Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ARGBP2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Rabbit Anti-ARGBP2 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS6463921

Flow Cytometry (FC/FACS) of anti-ARGBP2 antibody

Flow cytometric analysis of MDA-231 cells using ARGBP2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ARGBP2 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS6463921

Product References and Citations for anti-ARGBP2 antibody

1. Yuan, Z.Q., et.al., J. Biol. Chem. 280(22), 21483-21490 (2005). 2. Olsen, J.V., et.al., Cell 127(3), 635-648 (2006). 3. Taieb, D., et.al., Cancer Res. 68(12), 4588-4596 (2008).

Other Notes

Small volumes of anti-ARGBP2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ARGBP2. It may not necessarily be applicable to this product.

NCBI GI #
10947118
NCBI Accession #
NP_066547
UniProt Primary Accession #
O94875
GenBank Nucleotide #
NP_066547.1
NCBI Official Full Name
sorbin and SH3 domain-containing protein 2 isoform 2
UniProt Protein Name
Sorbin and SH3 domain-containing protein 2
UniProt Synonym Protein Names
Arg/Abl-interacting protein 2; ArgBP2; Sorbin
Protein Family
Sorbin and SH3 domain-containing protein
UniProt Gene Name
SORBS2
UniProt Synonym Gene Names
ARGBP2; KIAA0777; ArgBP2
UniProt Entry Name
SRBS2_HUMAN

UniProt Comments for ARGBP2

ArgBP2: Adapter protein that plays a role in the assembling of signaling complexes, being a link between ABL kinases and actin cytoskeleton. Can form complex with ABL1 and CBL, thus promoting ubiquitination and degradation of ABL1 or with AKT1 and PAK1, thus mediating AKT1-mediated activation of PAK1. Isoform 6 increases water and sodium absorption in the intestine and gall-bladder. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: perinuclear region of cytoplasm; plasma membrane; nucleus; Z disc; actin cytoskeleton

Molecular Function: protein domain specific binding; protein binding; structural constituent of cytoskeleton; structural constituent of muscle; metal ion binding; cytoskeletal adaptor activity

Biological Process: cell migration; actin filament organization; cell adhesion
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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