Rabbit Anti-ARHGEF1 Polyclonal Antibody – Cat# MBS6274942

ARHGEF1 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the ARHGEF1 antibody detected the ARHGEF1 protein (arrow).

ARHGEF1 Antibody – Rabbit Polyclonal

Name: ARHGEF1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS6274942
Price: 1060 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6274942

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple… More details

$1060 / 0.2 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

ARHGEF1, Polyclonal Antibody

Full Product Name

ARHGEF1, NT (ARHGEF1, Rho guanine nucleotide exchange factor 1, 115kD guanine nucleotide exchange factor, Sub1.5) (Biotin)

Product Gene Name

anti-ARHGEF1 antibody

Product Synonym Names

ARHGEF1; Rho guanine nucleotide exchange factor 1; 115kD guanine nucleotide exchange factor; Sub1.5

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

Purity/Purification

Purified by Protein A Affinity Chromatography.

Preparation and Storage

Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

OMIM

601855

3D Structure

ModBase 3D Structure for Q92888

Immunogen

ARHGEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-192 amino acids from the N-terminal region of human ARHGEF1.

Conjugate

Biotin

Note

Preservative Free

Product Categories/Family for anti-ARHGEF1 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

Applications Tested/Suitable for anti-ARHGEF1 antibody

Western Blot (WB), ELISA (EIA)

Application Notes for anti-ARHGEF1 antibody

WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.

Product References and Citations for anti-ARHGEF1 antibody

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Loirand, G., et al. Med Sci (Paris) 26 (6-7), 561-563 (2010): Takefuji, M., et al. J. Hum. Genet. 55(1):42-49(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Slattum, G., et al. J. Cell Biol. 186(5):693-702(2009).

Other Notes

Small volumes of anti-ARHGEF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ARHGEF1 purchase

MBS6274942 is an antibody targeting ARHGEF1. Also known as ARHGEF1, NT (ARHGEF1, Rho guanine nucleotide exchange factor 1, 115kD guanine nucleotide exchange factor, Sub1.5) (Biotin). Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ARHGEF1. It may not necessarily be applicable to this product.

NCBI GI #

39777604

NCBI GeneID

9138

NCBI Accession #

NP_945328.1

NCBI Related Accession #

NP_945353.1; NP_004697.2

UniProt Primary Accession #

Q92888

UniProt Related Accession #

Q92888

GenBank Nucleotide #

NP_945328.1

NCBI Official Full Name

rho guanine nucleotide exchange factor 1 isoform 3

NCBI Official Synonym Full Names

Rho guanine nucleotide exchange factor 1

NCBI Official Symbol

ARHGEF1

NCBI Official Synonym Symbols

LSC; GEF1; IMD62; LBCL2; SUB1.5; P115-RHOGEF

UniProt Protein Name

Rho guanine nucleotide exchange factor 1

UniProt Synonym Protein Names

115 kDa guanine nucleotide exchange factor; p115-RhoGEF; p115RhoGEF; Sub1.5

Protein Family

Rho guanine nucleotide exchange factor

UniProt Gene Name

ARHGEF1

UniProt Synonym Gene Names

p115-RhoGEF; p115RhoGEF

UniProt Entry Name

ARHG1_HUMAN

NCBI Protein Information

rho guanine nucleotide exchange factor 1

UniProt Comments for ARHGEF1

ARHGEF1: Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2- induced RhoA activation. Interacts with RHOA, GNA12 and GNA13. Homooligomerizes through the coiled coil region. May interact with CCPG1. Interacts with CTNNAL1. Ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GEFs, Rac/Rho; GEFs; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19q13.13

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: Rho guanyl-nucleotide exchange factor activity; protein binding; GTPase activator activity

Biological Process: cell proliferation; nerve growth factor receptor signaling pathway; negative regulation of axonogenesis; regulation of axonogenesis; Rho protein signal transduction

Research Articles on ARHGEF1

1. High ARHGEF1 expression is associated with asthmatic airway hyper-responsiveness.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.