• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ARHGEF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8109999

ARHGEF1 is a RhoA-specific guanine nucleotide exchange factor expressed in hematopoietic cells. ARHGEF1 regulates signaling by both G protein-coupled receptors and RhoA, and, consequently, is required for appropriate migration and adhesion of diverse… More details

$370 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ARHGEF1, Polyclonal Antibody
Full Product Name
Anti-ARHGEF1 Antibody, Rabbit Polyclonal
Product Gene Name
Product Synonym Names
ARHGEF1 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-GEF1 Antibody; Anti-LBCL2 Antibody; Anti-LSC Antibody; Anti-P115-RHOGEF Antibody; Anti-SUB1.5 Antibody
Clonality
Polyclonal
Isotype
Rabbit IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Purity/Purification
Protein A & Antigen Affinity
Specificity
Human ARHGEF1
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunogen
E Coli-derived Human ARHGEF1 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human ARHGEF1 fragment, and purified by antigen affinity chromatography.
Applications Tested/Suitable for anti-ARHGEF1 antibody
Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes for anti-ARHGEF1 antibody
IHC-P: 1:1000-1:5000
ICC/IF: 1:100-1:500

Immunohistochemistry (IHC) of anti-ARHGEF1 antibody

Immunochemical staining of human ARHGEF1 in human spleen with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.
Rabbit Anti-ARHGEF1 Polyclonal Antibody – Cat# MBS8109999

Immunofluorescence (IF) of anti-ARHGEF1 antibody

Immunofluorescence staining of ARHGEF1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ARHGEF1 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.
Rabbit Anti-ARHGEF1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS8109999

Product References and Citations for anti-ARHGEF1 antibody

Xiang X, et al. (2016) Arhgef1 negatively regulates neurite outgrowth through activation of rhoa signaling pathways. FEBS Lett 590 (17): 2940-2955.Brown JP, et al. (2007) Arhgef1 is required by t cells for the development of airway hyperreactivity and inflammation. Am J Respir Crit Care Med 176 (1): 10-19.Bouafia A, et al. (2019) Loss of arhgef1 causes a human primary antibody deficiency. J Clin Invest 129 (3): 1047-1060.

Other Notes

Small volumes of anti-ARHGEF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ARHGEF1 purchase

MBS8109999 is an antibody targeting ARHGEF1. Also known as Anti-ARHGEF1 Antibody, Rabbit Polyclonal. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ARHGEF1. It may not necessarily be applicable to this product.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000