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ATF3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS2400347

ATF3 Antibody Description: Activating transcription factor 3 (ATF3)is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. It encodes a protein with a calculated molecular… More details

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Product Overview

Product Name
ATF3, Polyclonal Antibody
Full Product Name
Anti-ATF3 Antibody (aa140-168) IHC-plus
Product Gene Name
anti-ATF3 antibody
Product Synonym Names
Rabbit Polyclonal to Human ATF3; Human ATF3
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, 0.09% sodium azide
Purity/Purification
Immunoaffinity purified
Specificity
Human ATF3
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months.
For long term storage store at -20 degree C.
Molecular Weight
14,314 Da
OMIM
603148
3D Structure
ModBase 3D Structure for P18847
Immunogen
ATF3 antibody was raised against kLH-conjugated synthetic peptide from C-terminal region of human ATF3.
Immunogen Description
KLH-conjugated synthetic peptide from C-terminal region of human ATF3.
Immunogen Type
Synthetic peptide
Antigen Modification
aa140-168
Target Species
Human
Applications Tested/Suitable for anti-ATF3 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes for anti-ATF3 antibody
Flow Cytometry (1:10 - 1:50)
IHC-P (1:100)
WB (1:1000)

Immunohistochemistry (IHC) of anti-ATF3 antibody

Anti-ATF3 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Rabbit Anti-ATF3 Polyclonal Antibody – Cat# MBS2400347

Western Blot (WB) of anti-ATF3 antibody

Western blot of ATF3 (arrow) using rabbit polyclonal ATF3 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ATF3 gene.
Rabbit Anti-ATF3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2400347

Western Blot (WB) of anti-ATF3 antibody

ATF3 Antibody western blot of CEM cell line lysates (35 ug/lane). The ATF3 antibody detected the ATF3 protein (arrow).
Rabbit Anti-ATF3 Polyclonal Antibody, Western Blot (WB) – Cat# MBS2400347

Flow Cytometry (FC/FACS) of anti-ATF3 antibody

ATF3 Antibody flow cytometry of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Rabbit Anti-ATF3 Polyclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS2400347

Other Notes

Small volumes of anti-ATF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ATF3 purchase

MBS2400347 is an antibody targeting ATF3. Also known as Anti-ATF3 Antibody (aa140-168) IHC-plus. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ATF3. It may not necessarily be applicable to this product.

NCBI GI #
95102481
NCBI GeneID
467
NCBI Accession #
NP_004015.4
UniProt Primary Accession #
P18847
UniProt Secondary Accession #
Q5VTZ2; Q6ICQ9; Q7Z566; Q8WYM6
UniProt Related Accession #
P18847
GenBank Nucleotide #
NM_004024.4
NCBI Official Synonym Full Names
activating transcription factor 3
NCBI Official Symbol
ATF3
UniProt Protein Name
Cyclic AMP-dependent transcription factor ATF-3
UniProt Synonym Protein Names
Activating transcription factor 3
Protein Family
Activating transcription factor
UniProt Gene Name
ATF3
UniProt Synonym Gene Names
cAMP-dependent transcription factor ATF-3
UniProt Entry Name
ATF3_HUMAN
NCBI Protein Information
cyclic AMP-dependent transcription factor ATF-3

NCBI Summary for ATF3

This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]
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UniProt Comments for ATF3

ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: nucleolus; nucleoplasm; nucleus

Molecular Function: identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription corepressor activity; transcription factor activity

Biological Process: cellular protein metabolic process; cellular response to amino acid starvation; gluconeogenesis; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; unfolded protein response
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Research Articles on ATF3

1. Taken together, these results suggest that ATF3 promotes the progression of human gliomas

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ATF3 antibody

 Reference Product  PubMed Publications
 JUN antibody  >222 publications with ATF3 and JUN
 ATF4 antibody  >78 publications with ATF3 and ATF4
 DDIT3 antibody  >66 publications with ATF3 and DDIT3
 ATF2 antibody  >27 publications with ATF3 and ATF2
 TP53 antibody  >20 publications with ATF3 and TP53
 JUNB antibody  >18 publications with ATF3 and JUNB
 SMAD3 antibody  >6 publications with ATF3 and SMAD3
 CREB1 antibody  >4 publications with ATF3 and CREB1
 MAPK8 antibody  >3 publications with ATF3 and MAPK8
 SMARCA4 antibody  >2 publications with ATF3 and SMARCA4

Pathways associated with anti-ATF3 antibody

 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 ATF4 Activates Genes Pathway antibodies  ATF4 Activates Genes Pathway Diagram
 Direct P53 Effectors Pathway antibodies  Direct P53 Effectors Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 Hypertrophy Model Pathway antibodies  Hypertrophy Model Pathway Diagram
 Id Signaling Pathway antibodies  Id Signaling Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Myometrial Relaxation And Contraction Pathways antibodies  Myometrial Relaxation And Contraction Pathways Diagram
 PERK Regulates Gene Expression Pathway antibodies  PERK Regulates Gene Expression Pathway Diagram

Diseases associated with anti-ATF3 antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >169 publications with ATF3 and Nervous System Diseases
 Disease Models, Animal Antibodies  >138 publications with ATF3 and Disease Models, Animal
 Pain Antibodies  >117 publications with ATF3 and Pain
 Inflammation Antibodies  >117 publications with ATF3 and Inflammation
 Peripheral Nervous System Diseases Antibodies  >81 publications with ATF3 and Peripheral Nervous System Diseases
 Cardiovascular Diseases Antibodies  >60 publications with ATF3 and Cardiovascular Diseases
 Necrosis Antibodies  >54 publications with ATF3 and Necrosis
 Lung Diseases Antibodies  >33 publications with ATF3 and Lung Diseases
 Liver Diseases Antibodies  >30 publications with ATF3 and Liver Diseases
 Breast Neoplasms Antibodies  >26 publications with ATF3 and Breast Neoplasms

Organs/Tissues associated with anti-ATF3 antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >182 publications with ATF3 and Nerve
 Ganglia Antibodies  >130 publications with ATF3 and Ganglia
 Blood Antibodies  >103 publications with ATF3 and Blood
 Brain Antibodies  >103 publications with ATF3 and Brain
 Liver Antibodies  >73 publications with ATF3 and Liver
 Muscle Antibodies  >69 publications with ATF3 and Muscle
 Bone Antibodies  >54 publications with ATF3 and Bone
 Vascular Antibodies  >51 publications with ATF3 and Vascular
 Lung Antibodies  >49 publications with ATF3 and Lung
 Skin Antibodies  >45 publications with ATF3 and Skin
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