B Myb/MYBL2 (MYBL2) Antibody – Rabbit Polyclonal

Figure 1. Western blot analysis of B MyB/MYBL2 using anti-B MyB/MYBL2 antibody (MBS1753536).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates
Lane 2: human HEK293 whole cell lysates
Lane 3: human Caco-2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-B MyB/MYBL2 antigen affinity purified polyclonal antibody (Catalog # MBS1753536) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for B MyB/MYBL2 at approximately 100KD. The expected band size for B MyB/MYBL2 is at 100KD.

Figure 2. IF analysis of B MyB/MYBL2 using anti- B MyB/MYBL2 antibody (MBS1753536).
B MyB/MYBL2 was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- B MyB/MYBL2 Antibody (MBS1753536) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Figure 3. Flow Cytometry analysis of U937 cells using anti-B MyB/MYBL2 antibody (MBS1753536).
Overlay histogram showing U937 cells stained with MBS1753536 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-B MyB/MYBL2 Antibody (MBS1753536, 1μg/1x10⁶ cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.