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BATF3 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS249671

MBS249671 is an antibody targeting BATF3. Also known as Anti-BATF3 Antibody (N-Terminus) IHC-plus. Reactive in Human. Host: Rabbit. More details

$650 / 0.05 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
BATF3, Polyclonal Antibody
Full Product Name
Anti-BATF3 Antibody (N-Terminus) IHC-plus
Product Gene Name
anti-BATF3 antibody
Product Synonym Names
Rabbit Polyclonal to Human BATF3; Human BATF3; JUNDM1; JDP1; Jun dimerization protein 1; B-ATF-3; SNFT
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
PBS, containing 0.02% sodium azide.
Purity/Purification
Peptide Column Affinity Chromatography
Concentration
1 mg/ml (lot specific)
Specificity
BATF3 antibody is human, mouse and rat reactive. BATF3 antibody is predicted to have no cross-reactivity to other BATF family members.
Preparation and Storage
Store at 4°C for up to 3 months. Store at -20°C for up to 1 year. Avoid repeated freeze/thaw cycles.
OMIM
612470
3D Structure
ModBase 3D Structure for Q9NR55
Immunogen Species
BATF3 antibody was raised against Human
Antigen Type
Synthetic peptide
Immunogen
BATF3 antibody was raised against a 16 amino acid peptide near the N-terminus of human BATF3. The immunogen is located within the first 50 amino acids of BATF3.
Disclaimer
Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
Recommended Immunohistochemistry Protocol
The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:

Tissue Preparation:
Formalin fixation and embedding in paraffin wax.

Tissue Sectioning:
Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C.

Deparaffinization:
Wash dry slides in 3 changes of xylene - 5 minutes each @ RT

Rehydration:
Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT
Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT
Wash slides in 1 change of 80% alcohol - 3 minutes @ RT
Rinse slides in gentle running distilled water - 5 minutes @ RT

Antigen retrieval:
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) -1 minute @ RT

Immunostaining:
(Do not allow tissues to dry at any time during the staining procedure)
Apply a universal protein block - 20 minutes @ RT
Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT
Rinse slides in 1 X TBST - 1 minute @ RT
Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase streptavidin - 30 minutes @ RT
Rinse slides in 1X TBST -1 minute @ RT
Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT
Wash slides in distilled water - 1 minute @ RT

Dehydrate:
(This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB)
Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT
Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT
Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT
Wash slides in 3 changes of xylene - 1 minute each @ RT
Apply coverslip
Applications Tested/Suitable for anti-BATF3 antibody
Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB), ELISA
Application Notes for anti-BATF3 antibody
IHC
IHC - Paraffin (10 ug/ml)
Immunofluorescence
Western Blot (1 - 2 ug/ml)
ELISA
(optimal dilutions to be determined by the researcher)

Usage Summary: BATF3 body can be used for detection of BATF3 by Western Blot at 0.5 ug/ml. Antibody can also be used for Immunohistochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not tested yet. Western Blot - Predicted: 14 kDa, Observed: 17 kDa

Immunohistochemistry (IHC) of anti-BATF3 antibody

Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Rabbit Anti-BATF3 Polyclonal Antibody – Cat# MBS249671

Other Notes

Small volumes of anti-BATF3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for BATF3. It may not necessarily be applicable to this product.

NCBI GI #
8924246
NCBI GeneID
55509
NCBI Accession #
NP_061134.1
UniProt Primary Accession #
Q9NR55
UniProt Related Accession #
Q9NR55
GenBank Nucleotide #
NM_018664.2
NCBI Official Full Name
basic leucine zipper transcriptional factor ATF-like 3
NCBI Official Synonym Full Names
basic leucine zipper transcription factor, ATF-like 3
NCBI Official Symbol
BATF3
NCBI Official Synonym Symbols
JDP1; SNFT; JUNDM1
UniProt Protein Name
Basic leucine zipper transcriptional factor ATF-like 3
UniProt Synonym Protein Names
21 kDa small nuclear factor isolated from T-cells; Jun dimerization protein p21SNFT
Protein Family
Basic leucine zipper transcriptional factor
UniProt Gene Name
BATF3
UniProt Synonym Gene Names
SNFT; B-ATF-3
UniProt Entry Name
BATF3_HUMAN
NCBI Protein Information
basic leucine zipper transcriptional factor ATF-like 3

NCBI Summary for BATF3

This gene encodes a member of the basic leucine zipper protein family. The encoded protein functions as a transcriptional repressor when heterodimerizing with JUN. The protein may play a role in repression of interleukin-2 and matrix metalloproteinase-1 transcription.[provided by RefSeq, Feb 2009]

UniProt Comments for BATF3

BATF3: Functions as a negative regulator of AP-1-mediated transcription by heterodimerizing with JUN and binding DNA at 12- O-tetradecanoylphorbol-13-acetate response elements (TRE) (consensus: 5'-TGA[CG]TCA-3'). Represses IL2 and MMP1 promoter activities. Belongs to the bZIP family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: nucleus

Molecular Function: protein binding; transcription corepressor activity; transcription factor activity

Biological Process: myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; response to virus; transcription from RNA polymerase II promoter
Show more

Research Articles on BATF3

1. Batf3-dependent dendritic cells elicit the rejection of cells and grafts expressing mismatched minor histocompatibility antigens.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BATF3 antibody

 Reference Product  PubMed Publications
 JUN antibody  >12 publications with BATF3 and JUN
 FOS antibody  >2 publications with BATF3 and FOS

Pathways associated with anti-BATF3 antibody

 Products by Pathway  Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram

Diseases associated with anti-BATF3 antibody

 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >11 publications with BATF3 and Disease Models, Animal
 Inflammation Antibodies  >11 publications with BATF3 and Inflammation
 Neoplasms, Experimental Antibodies  >5 publications with BATF3 and Neoplasms, Experimental
 Nervous System Diseases Antibodies  >5 publications with BATF3 and Nervous System Diseases
 Lung Diseases Antibodies  >3 publications with BATF3 and Lung Diseases
 Necrosis Antibodies  >3 publications with BATF3 and Necrosis
 Liver Diseases Antibodies  >2 publications with BATF3 and Liver Diseases
 Fibrosis Antibodies  >2 publications with BATF3 and Fibrosis
 Kidney Diseases Antibodies  >1 publications with BATF3 and Kidney Diseases
 Adenocarcinoma Antibodies  >1 publications with BATF3 and Adenocarcinoma

Organs/Tissues associated with anti-BATF3 antibody

 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >7 publications with BATF3 and Lung
 Brain Antibodies  >2 publications with BATF3 and Brain
 Thymus Antibodies  >2 publications with BATF3 and Thymus
 Pancreas Antibodies  >1 publications with BATF3 and Pancreas
 Connective Tissue Antibodies  >1 publications with BATF3 and Connective Tissue
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